Life cycle and spore resistance of spore-forming Bacillus atrophaeus

Sella, Sandra Regina B. R.; Vandenberghe, Luciana Porto de Souza; Soccol, Carlos Ricardo

doi:10.1016/j.micres.2014.05.001

Cited by 99 publications

(53 citation statements)

References 84 publications

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“…The B. pumilus species belonging to the B. subtilis group is ubiquitous both in the terrestrial and marine ecosystems. This bacterium is highly resistant to extreme environmental conditions, such as low or no nutrient availability, desiccation, irradiation and chemical disinfections (Fritze, 2004;Menezes et al, 2010;Sella, Vandenberghe, & Soccol, 2014). There are several previous reports confirming that some B. pumilus strains are capable of causing diseases in pears (Li et al, 2009), peaches (Saleh, Huang, & Huang, 1997), mangoes (Galal, El-Bana, & Janse, 2006), Ficus lacor (Hakim, Liaquat, Gul, Chaudhary, & Munis, 2015) and pine tree (Kovaleva, Shalovylo, et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Bacillus pumilus and Stenotrophomonas maltophilia as two potentially causative agents involved in Persian oak decline in Zagros forests (Iran)

et al. 2019

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The oak decline is known as one of the most destructive complex diseases causing high economic losses around the world, especially in Iran. The main objective of the present study was to investigate the possible role of bacteria as causative agents of oak decline in the Zagros forests of Iran. To do this, stem, root and leaf samples were taken from symptomatic Persian oak trees (Quercus brantii) in different zones of Zagros forests (Ilam Province, Iran). From 150 bacterial isolates, 20 showed pathogenicity against Geranium seedlings. Among 20 hypersensitivity test positive strains, four strains showed pathogenicity against oak saplings. Based on morphological and 16S rRNA gene sequence analysis, three strains were identified as Bacillus pumilus and one strain as non‐sporulating Gram‐negative Stenotrophomonas maltophilia. Pathogenicity studies of different B. pumilus and S. maltophilia strains revealed that they have potential to cause the disease in oak saplings and symptoms of disorder in Persian oak trees. To our knowledge, there are no previous records of B. pumilus and S. maltophilia causing decline on Fagaceous trees like Q. brantii. More detailed field and molecular studies are required to confirm the absolute role of such bacteria in occurrence of oak decline in Zagros forests.

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Section: Discussionmentioning

confidence: 99%

Bacillus pumilus and Stenotrophomonas maltophilia as two potentially causative agents involved in Persian oak decline in Zagros forests (Iran)

et al. 2019

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“…The heat causes damages in bacterial spore cells, and the heated spores’ deformations and damages, and the release of intracellular components (Rozali et al ). However, they still have the ability to germinate after sublethal injury, and their suitability for genetic manipulation are of practical importance for food sterilization processes (Sella et al ). Our results also showed that just alone thermal processing could not kill all bacteria or inhibit bacterial growth and it required multiple methods to work together.…”

Section: Discussionmentioning

confidence: 99%

Combined propidium monoazide pretreatment with high‐throughput sequencing evaluated the bacterial diversity in chicken skin after thermal treatment

et al. 2019

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Aims The purpose of this experiment was to study the bacterial diversity and predominance of spoilage bacteria in chicken skin at different thermal treatment temperatures (60, 70, 80, 90, 100, 110, 120°C). Method and Results Bacteria in chicken skin was collected, then propidium monoazide treatment to remove the DNA of dead cell, total DNA was extracted by Tiandz Bacterial DNA Kit, and investigated by high‐throughput sequencing of the v3/v4 regions of the 16S rDNA gene. A total of 796 008 high‐quality bacterial sequences were obtained for assessing the microbial diversity of chicken skin from seven thermal treatment group and control group. The results showed that the bacterial diversity in chicken skin at 90°C was lowest. And Acinetobacter (25·88%), Clostridium (20·70%), Bacteroides (13·93%) and Myroides (13·13%) were the main flora at 25°C; The Clostridium was dominant genus of the samples heat‐treated by 60, 70, 80 and 90°C, the proportion of this genus were up to 64·86, 77·42, 52·22 and 87·30% respectively. The Bacillus was the main flora of the samples heat‐treated by 100, 110 and 120°C, and the relative percentages were 39·44, 79·61 and 45·96% respectively. In addition, high‐temperature‐resistant Serratia was found in chicken skin. Conclusions The study revealed that the relationship between thermal treatment temperature and bacterial diversity and dominant spoilage bacteria in chicken skin, which had a strong guiding significance for the control and prediction of micro‐organisms in foods. Significance and Impact of the Study The results of this paper could provide a theoretical basis for meat products containing chicken skin, including the safe use of chicken skin, determination of sterilization process parameters and selection of preservatives for compounding, which has strong practicality in China.

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“…Bacillus-based probiotics present an advantage because of the inherent resistance of Bacillus spores (40). Although the Bacillus clausii preparation Enterogermina has been described as an antidiarrheal that also prevents antibiotic-associated diarrhea (28,29), data describing the functional mechanisms remain very limited.…”

Section: Discussionmentioning

confidence: 99%

Secreted Compounds of the Probiotic Bacillus clausii Strain O/C Inhibit the Cytotoxic Effects Induced by Clostridium difficile and Bacillus cereus Toxins

Ripert

Racedo

Elie

et al. 2016

Antimicrob Agents Chemother

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Although the use of probiotics based on Bacillus strains to fight off intestinal pathogens and antibiotic-associated diarrhea is widespread, the mechanisms involved in producing their beneficial effects remain unclear. Here, we studied the ability of compounds secreted by the probiotic Bacillus clausii strain O/C to counteract the cytotoxic effects induced by toxins of two pathogens, Clostridium difficile and Bacillus cereus, by evaluating eukaryotic cell viability and expression of selected genes. Coincubation of C. difficile and B. cereus toxic culture supernatants with the B. clausii supernatant completely prevented the damage induced by toxins in Vero and Caco-2 cells. The hemolytic effect of B. cereus was also avoided by the probiotic supernatant. Moreover, in these cells, the expression of rhoB, encoding a Rho GTPase target for C. difficile toxins, was normalized when C. difficile supernatant was pretreated using the B. clausii supernatant. All of the beneficial effects observed with the probiotic were abolished by the serine protease inhibitor phenylmethylsulfonyl fluoride (PMSF). Suspecting the involvement of a secreted protease in this protective effect, a protease was purified from the B. clausii supernatant and identified as a serine protease (M-protease; GenBank accession number Q99405). Experiments on Vero cells demonstrated the antitoxic activity of the purified protease against pathogen supernatants. This is the first report showing the capacity of a protease secreted by probiotic bacteria to inhibit the cytotoxic effects of toxinogenic C. difficile and B. cereus strains. This extracellular compound could be responsible, at least in part, for the protective effects observed for this human probiotic in antibiotic-associated diarrhea.T he gut, one of the biggest surfaces of exchange between the body's internal and external environment, is in permanent contact with antigens. Indeed, the intestinal barrier constitutes an important playground for numerous intestinal pathogen bacteria and toxins.Among them, Clostridium difficile, an anaerobic Gram-positive bacterium, is a major cause of diarrhea, pseudomembranous colitis, and septicemia, and it can lead to death. Hospital stay and use of antibiotics are major risk factors for contracting this disease (1). Over the past decade, the frequency and severity of C. difficile infections have increased markedly due to the emergence of socalled hypervirulent strains that overproduce toxins (2-4). The C. difficile toxins modify target cell proteins to cause disassembly of the actin cytoskeleton and induce severe inflammation (5).Expression of several host genes, mainly those involved in cellular processes, cell-cell interactions, apoptosis, and inflammation, is modified during C. difficile infection (6). These include host genes encoding RhoB (regulating actin cytoskeleton and interactions between cells), ZO-1 (tight-junction protein), and ROCK2 (actin cytoskeleton and signaling protein regulator) (7-9).Equally, many other pathogens, such as Salmonella species, E...

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Life cycle and spore resistance of spore-forming Bacillus atrophaeus

Cited by 99 publications

References 84 publications

Bacillus pumilus and Stenotrophomonas maltophilia as two potentially causative agents involved in Persian oak decline in Zagros forests (Iran)

Bacillus pumilus and Stenotrophomonas maltophilia as two potentially causative agents involved in Persian oak decline in Zagros forests (Iran)

Combined propidium monoazide pretreatment with high‐throughput sequencing evaluated the bacterial diversity in chicken skin after thermal treatment

Secreted Compounds of the Probiotic Bacillus clausii Strain O/C Inhibit the Cytotoxic Effects Induced by Clostridium difficile and Bacillus cereus Toxins

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