Nowadays, industries across the globe are acknowledging the need for a Net Zero Supply Chain (NZSC) by 2050, particularly within the Manufacturing Supply Chain (MSC) due to its significant contribution to Greenhouse Gas (GHG) emissions. Government and regulatory bodies pressure the industry to reduce GHG emissions. Industries focus on cleaner and sustainable production by adopting net zero technique practices. Achieving the net zero goal needs technological integration, such as Caron Capture Technology (CCT) and Life Cycle Assessment (LCA). Are LCA and CCT unlocking the potential for net-zero practices in MSC? This still needs to be answered. Therefore, this study aims to identify and prioritize potential enablers of Life Cycle Assessment (LCA) to facilitate the NZSC. We identified potential enablers using a Systematic Literature Review (SLR) and expert opinion. An empirical study validated these enablers, followed by the application of the “Best-Worst Method (BWM)” to rank them and the “Fuzzy Decision-making trial and evaluation laboratory (F-DEMATEL)” to identify causal relationships. The outcomes of this study reveal that ‘Energy Management and Emission Reduction’ is the most critical category of enablers, followed by ‘Governance and Collaboration’, ‘Assessment and Digitalization’, and ‘Sustainable Materials and Production’. Outcomes highlight the importance of integrating technological innovations and stakeholder engagement to achieve net-zero goals. The implications of this study provide valuable insights for policymakers and practitioners. By focusing on the prioritized enablers, the manufacturing industry can effectively implement LCA, develop the NZSC business model, and enhance competitiveness in the global market. This study contributes to the ongoing discourse on sustainable manufacturing practices and offers a strategic framework for achieving NZSC by 2050.