Life Cycle of White Blood Cells

Lawrence, John S.; Ervin, Donald M.; Wetrich, Raymond M.

doi:10.1152/ajplegacy.1945.144.2.284

Cited by 31 publications

(1 citation statement)

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“…Using an in vivo radioactive labelling methodology, Pillay et al 17 estimated that mouse neutrophils mature during 2.3 days (5.8 days in human) within the bone marrow, before they get released and circulate for 0.75 days (5.4 days in humans). The results regarding the bone marrow maturation time obtained with this approach corresponded to previous studies 18,19 , however the peripheral transit time was 10 times greater than previously estimated for human neutrophils [20][21][22] . According to Pillay et al, these latter approaches required ex vivo labelling, which may have impacted their activation status and circulating time in blood.…”

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confidence: 86%

Durable and controlled depletion of neutrophils in mice

et al. 2020

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Neutrophils are an essential part of the innate immune system. To study their importance, experimental studies often aim to deplete these cells, generally by injecting anti-Ly6G or anti-Gr1 antibodies. However, these approaches are only partially effective, transient or lack specificity. Here we report that neutrophils remaining after anti-Ly6G treatment are newly derived from the bone marrow, instead of depletion escapees. Mechanistically, newly generated, circulating neutrophils have lower Ly6G membrane expression, and consequently reduced targets for anti-Ly6G-mediated depletion. To overcome this limitation, we develop a double antibody-based depletion strategy that enhances neutrophil elimination by anti-Ly6G treatment. This approach achieves specific, durable and controlled reduction of neutrophils in vivo, and may be suitable for studying neutrophil function in experimental models.

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confidence: 86%