Life history of oysters influences Vibrio parahaemolyticus accumulation in Pacific oysters (Crassostrea gigas)

Abstract

Summary Vibrio parahaemolyticus infection in humans is associated with raw oyster consumption. Evaluation of V. parahaemolyticus presence in oysters is of most interest because of the economic and public health issues that it represents. To explore V. parahaemolyticus accumulation and depuration in adult Crassostrea gigas, we developed a GFP‐tagged V. parahaemolyticus strain (IFVp201‐gfp+), as well as a rapid and efficient quantification method in C. gigas oysters haemolymph by flow cytometry. Impact of the life history of C. gigas on accumulation and depuration of V. parahaemolyticus IFVp201 was subsequently investigated. We found that naive oysters, i.e. grown in controlled facilities with UV treated seawater, accumulated significantly more IFVp201 than environmental oysters, i.e. grown in intertidal environment. We hypothesized that environmental oysters could have been immune primed, thus could limit V. parahaemolyticus accumulation. Meanwhile, both naive and environmental oysters had similar depuration rates.

Genetic and physiological insights into the diazotrophic activity of a non‐cyanobacterial marine diazotroph

“…Improving the electroporation efficiency will require trials and 14622920, ja, Downloaded from https://sfamjournals.onlinelibrary.wiley.com/doi/10.1111/1462-2920.16261 by IFREMER Centre Bretagne BLP, Wiley Online Library on [02/11/2022]. See the Terms and Conditions (https://onlinelibrary.wiley.com/terms-and-conditions) on Wiley Online Library for rules of use; OA articles are governed by the applicable Creative Commons License errors, but having an already functional method reinforce its high potential for rapid introduction of exogenous DNA, strengthened by its functionality in other marine bacteria like Algoriphagus machipongonensis (Xu et al, 2022) or human pathogens like Vibrio parahaemolyticus (Soree et al, 2022). Conjugation was more efficient, both for the transfer of replicative plasmids and for the insertion of mini-transposons.…”

“…Electroporation efficiency was albeit low, as compared with the one reported for other Vibrio or Pseudoalteromonas species (Delavat et al, 2018 ). Improving the electroporation efficiency will require trials and errors, but having an already functional method reinforce its high potential for rapid introduction of exogenous DNA, strengthened by its functionality in other marine bacteria like Algoriphagus machipongonensis (Xu et al, 2022 ) or human pathogens like Vibrio parahaemolyticus (Soree et al, 2022 ). Conjugation was more efficient, both for the transfer of replicative plasmids and for the insertion of mini‐transposons.…”

Genetic and physiological insights into the diazotrophic activity of a non-cyanobacterial marine diazotroph

“…Electroporation efficiency was albeit low, as compared to the one reported for other Vibrio or Pseudoalteromonas species (Delavat et al, 2018). Improving the electroporation efficiency will require trials and errors, but having an already functional method reinforce its high potential for rapid introduction of exogenous DNA, strengthened by its functionality in other marine bacteria like Algoriphagus machipongonensis (Xu et al, 2022) or human pathogens like Vibrio parahaemolyticus (Soree et al, 2022). Conjugation was more efficient, both for the transfer of replicative plasmids and for the insertion of mini-transposons.…”