Life in soil by the actinorhizal root nodule endophyte Frankia. A review

Chaia, Eugenia; Wall, Luis Gabriel; Huss‐Danell, Kerstin

doi:10.1007/s13199-010-0086-y

Cited by 102 publications

(69 citation statements)

References 187 publications

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“…Frankia strain CcI3 was grown and maintained in basal MP growth medium with 5.0 mM NH 4 Cl as a nitrogen source and 5.0 mM propionate as a carbon source at 28°C, as described previously (33,34). For some experiments, cells were grown in BAP medium as described previously (26).…”

Section: Methodsmentioning

confidence: 99%

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Casuarina Root Exudates Alter the Physiology, Surface Properties, and Plant Infectivity of Frankia sp. Strain CcI3

Beauchemin

Furnholm

Lavenus

et al. 2012

Appl Environ Microbiol

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The actinomycete genus Frankia forms nitrogen-fixing symbioses with 8 different families of actinorhizal plants, representing more than 200 different species. Very little is known about the initial molecular interactions between Frankia and host plants in the rhizosphere. Root exudates are important in Rhizobium-legume symbiosis, especially for initiating Nod factor synthesis. We measured differences in Frankia physiology after exposure to host aqueous root exudates to assess their effects on actinorhizal symbioses. Casuarina cunninghamiana root exudates were collected from plants under nitrogen-sufficient and -deficient conditions and tested on Frankia sp. strain CcI3. Root exudates increased the growth yield of Frankia in the presence of a carbon source, but Frankia was unable to use the root exudates as a sole carbon or energy source. Exposure to root exudates caused hyphal "curling" in Frankia cells, suggesting a chemotrophic response or surface property change. Exposure to root exudates altered Congo red dye binding, which indicated changes in the bacterial surface properties at the fatty acid level. Fourier transform infrared spectroscopy (FTIR) confirmed fatty acid changes and revealed further carbohydrate changes. Frankia cells preexposed to C. cunninghamiana root exudates for 6 days formed nodules on the host plant significantly earlier than control cells. These data support the hypothesis of early chemical signaling between actinorhizal host plants and Frankia in the rhizosphere.A ctinorhizal symbioses are mutualistic interactions that occur between actinorhizal plants and the actinomycete genus Frankia (27,38). Frankia exists either in a free-living state in the soil or in symbiosis with actinorhizal plants (2, 4, 31). Actinorhizal plants consist of 8 different plant families, including over 200 different species of woody dicotyledonous trees and shrubs (31,38,42). The globally distributed actinorhizal plants are found on every continent except Antarctica and are able to grow in a diverse set of natural habitats, including arid lands, plains, tundra, and temperate forests. Actinorhizal plants are pioneer plant species that are able to grow in extremely nutrient-poor soil conditions and, with the aid of Frankia, are able to reclaim surrounding soil in disrupted environments. Actinorhizal plants are important in agroforestry, in soil reclamation, and as a fuel source.While actinorhizal symbiosis has been well studied at a morphological level since the 1970s, very little is known about the molecular interactions that occur between the plant hosts and Frankia during the establishment of the association. The establishment of the symbiosis encompasses the infection and nodulation processes (23,27,38). In general, the bacterium needs to recognize a host plant and the host needs to identify the bacterium as a friend, not a foe. The bacteria enter the plant and establish the association after several steps. The infection ultimately leads to the formation of the mature nodule.There is a paucity of information o...

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Section: Methodsmentioning

confidence: 99%

“…Frankia exists either in a free-living state in the soil or in symbiosis with actinorhizal plants (2,4,31). Actinorhizal plants consist of 8 different plant families, including over 200 different species of woody dicotyledonous trees and shrubs (31,38,42).…”

mentioning

confidence: 99%

Casuarina Root Exudates Alter the Physiology, Surface Properties, and Plant Infectivity of Frankia sp. Strain CcI3

Beauchemin

Furnholm

Lavenus

et al. 2012

Appl Environ Microbiol

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“…Frankia bacteria are mycelium-forming actinomycetes that are found as nitrogen-fixing symbionts in the root nodules of angiosperm plant species but can also survive as free-living soil bacteria (9,14). These bacteria are developmentally complex, forming three cell types: vegetative hyphae, spores located in sporangia, and unique lipid-enveloped cellular structures called vesicles.…”

mentioning

confidence: 99%

Significant Natural Product Biosynthetic Potential of Actinorhizal Symbionts of the Genus Frankia, as Revealed by Comparative Genomic and Proteomic Analyses

Udwary

Gontang

Jones

et al. 2011

Appl Environ Microbiol

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Bacteria of the genus Frankia are mycelium-forming actinomycetes that are found as nitrogen-fixing facultative symbionts of actinorhizal plants. Although soil-dwelling actinomycetes are well-known producers of bioactive compounds, the genus Frankia has largely gone uninvestigated for this potential. Bioinformatic analysis of the genome sequences of Frankia strains ACN14a, CcI3, and EAN1pec revealed an unexpected number of secondary metabolic biosynthesis gene clusters. Our analysis led to the identification of at least 65 biosynthetic gene clusters, the vast majority of which appear to be unique and for which products have not been observed or characterized. More than 25 secondary metabolite structures or structure fragments were predicted, and these are expected to include cyclic peptides, siderophores, pigments, signaling molecules, and specialized lipids. Outside the hopanoid gene locus, no cluster could be convincingly demonstrated to be responsible for the few secondary metabolites previously isolated from other Frankia strains. Few clusters were shared among the three species, demonstrating species-specific biosynthetic diversity. Proteomic analysis of Frankia sp. strains CcI3 and EAN1pec showed that significant and diverse secondary metabolic activity was expressed in laboratory cultures. In addition, several prominent signals in the mass range of peptide natural products were observed in Frankia sp. CcI3 by intact-cell matrix-assisted laser desorption-ionization mass spectrometry (MALDI-MS). This work supports the value of bioinformatic investigation in natural products biosynthesis using genomic information and presents a clear roadmap for natural products discovery in the Frankia genus.

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“…In addition, the walls of these structures are composed of lipid layers which act as a barrier to ambient oxygen levels, and permit to avoid the inhibition of nitrogenase activity. Actinorhizal plants comprise 200 plant species belonging to 25 genera of woody plants from eight non-leguminous dicotyledonous angiosperm families Casuarinaceae, Betulaceae, Rhamnaceae, Rosaceae, Myricaceae, Coriariaceae, Elaeagnaceae and Datiscaceae (Benson and Clawson 2000;Pawlowski and Sirrenberg 2003;Chaia et al 2010). They are considered as early successional species following deglaciation (Chapin et al 1994) or fires (Roy et al 2007) and thus be used for conditioning soils for revegetation (Roy et al 2007).…”

Section: Symbiotic Actinobacteria: Genus Frankiamentioning

confidence: 99%

Diversity of Plant Associated Actinobacteria

Bouizgarne

Aouamar

2014

Sustainable Development and Biodiversity

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The phylum Actinobacteria encompasses Gram-positive bacteria with a high DNA G+C. In soils, they present multiple lifestyles: rhizosphere saprophytes, endophytes, facultative symbionts and obligate phytopathogens. They play either beneficial or adverse effects towards plants. Numerous studies focused on their taxonomy and preservation. Also, adequate strategies were developed to enable their isolation. Phenotypically, the Actinobacteria is one of the most diverse phyla within bacteria. Their presence was once monitored by classical culture dependent techniques and phenetic characterization. Development of culture independent methods including chemotaxonomic and genomic approaches such as 16S rRNA gene analysis allowed to detect the so called unculturable bacteria. Major works on their diversity combine culture dependant and independant techniques. This chapter focuses on the phenetic and genetic diversity of Actinobacteria in plant-soil systems. It begins with some knowledge of their biology and their taxonomy, followed by a brief overview of the methods that have facilitated advances in the understanding of their diversity. It also discusses diversity of ecologically important plant associated Actinobacteria (rhizosphere and phyllosphere colonizers, endophytes, symbionts and phytopathogens).

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Life in soil by the actinorhizal root nodule endophyte Frankia. A review

Cited by 102 publications

References 187 publications

Casuarina Root Exudates Alter the Physiology, Surface Properties, and Plant Infectivity of Frankia sp. Strain CcI3

Casuarina Root Exudates Alter the Physiology, Surface Properties, and Plant Infectivity of Frankia sp. Strain CcI3

Significant Natural Product Biosynthetic Potential of Actinorhizal Symbionts of the Genus Frankia, as Revealed by Comparative Genomic and Proteomic Analyses

Diversity of Plant Associated Actinobacteria

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