Life-Style and Genome Structure of Marine Pseudoalteromonas Siphovirus B8b Isolated from the Northwestern Mediterranean Sea

Lara, Elena; Holmfeldt, Karin; Solonenko, Natalie; Sà, Elisabet Laia; Ignacio‐Espinoza, J. Cesar; Cornejo‐Castillo, Francisco M.; VerBerkmoes, Nathan C.; Vaqué, Dolors; Acinas, Silvia G.

doi:10.1371/journal.pone.0114829

Cited by 13 publications

(10 citation statements)

References 126 publications

(153 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1c, d ) a member of the Vibrio splendidus clade, the most abundant group of marine vibrios in coastal seawaters 25 , and the relatively “fast” degrader, Pseudoalteromonas sp . palte3D05, a common member of heterotrophic bacterioplankton communities 26 , 27 . Secondary consumers, or strains unable to degrade chitin, have previously been found to invade particle-attached communities and to become numerically dominant during community assembly 17 (Fig.…”

Section: Resultsmentioning

confidence: 99%

Microscale ecology regulates particulate organic matter turnover in model marine microbial communities

et al. 2018

View full text Add to dashboard Cite

The degradation of particulate organic matter in the ocean is a central process in the global carbon cycle, the mode and tempo of which is determined by the bacterial communities that assemble on particle surfaces. Here, we find that the capacity of communities to degrade particles is highly dependent on community composition using a collection of marine bacteria cultured from different stages of succession on chitin microparticles. Different particle degrading taxa display characteristic particle half-lives that differ by ~170 h, comparable to the residence time of particles in the ocean’s mixed layer. Particle half-lives are in general longer in multispecies communities, where the growth of obligate cross-feeders hinders the ability of degraders to colonize and consume particles in a dose dependent manner. Our results suggest that the microscale community ecology of bacteria on particle surfaces can impact the rates of carbon turnover in the ocean.

show abstract

Section: Resultsmentioning

confidence: 99%

Microscale ecology regulates particulate organic matter turnover in model marine microbial communities

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Examples of mobile elements in Pseudoalteromonas includes ϕRIO phage in P. marina mano4 , B8b phage in Pseudoalteromonas sp. QC‐44 , phage PH357 in P. lipolytica BH357 and the plasmids pMBL6842 and pSM429 harbored by P. rubra SCSIO 6842 and Pseudoalteromonas sp. BSi20429 respectively (Supporting Information Table S11).…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial‐producing Pseudoalteromonas from the marine environment of Panama shows a high phylogenetic diversity and clonal structure

et al. 2018

View full text Add to dashboard Cite

Pseudoalteromonas is a genus of marine bacteria often found in association with other organisms. Although several studies have examined Pseudoalteromonas diversity and their antimicrobial activity, its diversity in tropical environments is largely unexplored. We investigated the diversity of Pseudoalteromonas in marine environments of Panama using a multilocus phylogenetic approach. Furthermore we tested their antimicrobial capacity and evaluated the effect of recombination and mutation in shaping their phylogenetic relationships. The reconstruction of clonal relationships among 78 strains including 15 reference Pseudoalteromonas species revealed 43 clonal lineages, divided in pigmented and non-pigmented strains. In total, 39 strains displayed moderate to high activity against Gram-positive and Gram-negative bacteria and fungi. Linkage disequilibrium analyses showed that the Pseudoalteromonas strains of Panama have a highly clonal structure and that, although present, recombination is not frequent enough to break the association among alleles. This clonal structure is in contrast to the high rates of recombination generally reported for aquatic and marine bacteria. We propose that this structure is likely due to the symbiotic association with marine invertebrates of most strains analyzed. Our results also show that there are several putative new species of Pseudoalteromonas in Panama to be described.

show abstract

“…Here we employ time resolved, genome-wide transcriptomics, and proteomics to better understand virocell metabolic reprogramming and ecology through Pseudoalteromonas phage-host interactions. The marine bacterium Pseudoalteromonas and its phages offer a tractable phage-host model system to study virocell ecology because genomic and life history traits of several Pseudoalteromonas hosts and phages have been described [35][36][37][38][39]. As marine Pseudoalteromonas spp.…”

Section: Introductionmentioning

confidence: 99%

Phage-specific metabolic reprogramming of virocells

et al. 2020

Self Cite

View full text Add to dashboard Cite

Ocean viruses are abundant and infect 20-40% of surface microbes. Infected cells, termed virocells, are thus a predominant microbial state. Yet, virocells and their ecosystem impacts are understudied, thus precluding their incorporation into ecosystem models. Here we investigated how unrelated bacterial viruses (phages) reprogram one host into contrasting virocells with different potential ecosystem footprints. We independently infected the marine Pseudoalteromonas bacterium with siphovirus PSA-HS2 and podovirus PSA-HP1. Time-resolved multi-omics unveiled drastically different metabolic reprogramming and resource requirements by each virocell, which were related to phage-host genomic complementarity and viral fitness. Namely, HS2 was more complementary to the host in nucleotides and amino acids, and fitter during infection than HP1. Functionally, HS2 virocells hardly differed from uninfected cells, with minimal host metabolism impacts. HS2 virocells repressed energy-consuming metabolisms, including motility and translation. Contrastingly, HP1 virocells substantially differed from uninfected cells. They repressed host transcription, responded to infection continuously, and drastically reprogrammed resource acquisition, central carbon and energy metabolisms. Ecologically, this work suggests that one cell, infected versus uninfected, can have immensely different metabolisms that affect the ecosystem differently. Finally, we relate phage-host genome complementarity, virocell metabolic reprogramming, and viral fitness in a conceptual model to guide incorporating viruses into ecosystem models.

show abstract

Life-Style and Genome Structure of Marine Pseudoalteromonas Siphovirus B8b Isolated from the Northwestern Mediterranean Sea

Cited by 13 publications

References 126 publications

Microscale ecology regulates particulate organic matter turnover in model marine microbial communities

Microscale ecology regulates particulate organic matter turnover in model marine microbial communities

Antimicrobial‐producing Pseudoalteromonas from the marine environment of Panama shows a high phylogenetic diversity and clonal structure

Phage-specific metabolic reprogramming of virocells

Contact Info

Product

Resources

About