Life with Tracers

Ussing, Hans H.

doi:10.1146/annurev.ph.42.030180.000245

Cited by 13 publications

(6 citation statements)

References 22 publications

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“…Ussing joined August Krogh ' s Laboratory of Zoophysiology at the University of Copenhagen in 1934, shortly after Harold C. Urey had given Krogh a small sample of heavy water ( Ussing, 1980 ). Ussing was recruited to assist Krogh in exploring the uses of heavy water in biology.…”

Section: B a C K G R O U N Dmentioning

confidence: 99%

“…c o r n e l l . e d u to pursue studies of transmembrane ion movement due to the ready availability of suitable tracers, which since 1938 had been produced by the cyclotron at the Niels Bohr Institute in Copenhagen ( Hahn et al, 1939 ;Ussing, 1980 ). Although, or maybe because, Krogh ' s focus was on K + , Ussing decided to examine the transmembrane movement of Na + in the search for evidence for the " sodium pump " that had been proposed, in various formulations, by earlier workers (cf.…”

Section: B a C K G R O U N Dmentioning

confidence: 99%

“…Not only was it necessary to abandon the muscle preparation, but to fully characterize the fl uxes it was also necessary to know both the transmembrane concentration and potential differences. This led Ussing to the isolated frog skin -in part because Krogh earlier had demonstrated that frogs can take up both Na + and Cl Ϫ from very dilute solutions ( Ussing, 1980 ).…”

Section: B a C K G R O U N Dmentioning

confidence: 99%

See 2 more Smart Citations

The Two-Membrane Model of Epithelial Transport: Koefoed-Johnsen and Ussing (1958)

Palmer

Andersen

2008

The Journal of General Physiology

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Section: B a C K G R O U N Dmentioning

confidence: 99%

Section: B a C K G R O U N Dmentioning

confidence: 99%

Section: B a C K G R O U N Dmentioning

confidence: 99%

See 1 more Smart Citation

The Two-Membrane Model of Epithelial Transport: Koefoed-Johnsen and Ussing (1958)

Palmer

Andersen

2008

The Journal of General Physiology

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“…Since these early studies, ouabain and strophanthidin have been widely utilized as Na pump inhibitors in a wide variety of cells and tissues. The fraction of Na efflux in muscle sensitive to external Na has been termed "exchange diffusion" ; this will be discussed in a separate section [97,135]. In frog sartorius muscle, the fraction of Na efflux abolished by Na-free solutions increases as [Na]i is made low [123].…”

Section: The Na Pump In Muscle Fibersmentioning

confidence: 99%

“…The hypothesis of a one-for-one Na ion exchange that occurs across the muscle fiber membrane was first proposed by Ussing [135]. According to this hypothesis, a carrier molecule exists in the membrane of muscle fibers that has a high specificity and a high affinity for Na ions.…”

Section: Exchange Diffusion Of Sodiummentioning

confidence: 99%

Transport of electrolytes in muscle

Sjodin

1982

J. Membrain Biol.

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The muscle fiber stands alongside the red blood cell and the giant axon as one of the three classical cell types that have had major application in investigating ion transport processes in cell membranes. Of these three cell types, the muscle fiber was the first to provide definite evidence for a sodium pump. The ability of the sodium pump to produce an electrical potential difference across the cell membrane was also first demonstrated in muscle fibers. This important property of the sodium pump is now known to have physiological significance in many other types of cells. In this review, electrolyte transport investigations in skeletal muscle are traced from their inception to the current state of the field. Applications of major research techniques are discussed and key results are summarized. An overview of electrolyte transport in muscle, this article emphasizes relationships between the muscle fiber membrane potential and ionic transport processes.

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Transport of exogenous organic substances by invertebrate integuments: The field revisited

Gomme

2001

J. Exp. Zool.

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The notion that some marine invertebrates can use integumental uptake of organic compounds as a nutritional supplement dates back to the late 19th and early 20th centuries. This article provides a brief overview of more than a century’s research, as it relates to significant stages in the development of experimental methods and concepts of general physiology. Emphasis is placed on changing paradigms and on the interplay between this specialized field of investigation and the mainstream of physiological thought. The present status of the field is summarized. The general consensus is challenged on the basis of previously published and new data from the author’s laboratory. Particular emphasis is placed on data pointing toward an ultra‐rapid turnover of amino acids in part of the epidermal space of the polychaete worm Nereis diversicolor. It is suggested that intra‐epidermal L‐alanine is compartmentalized metabolically or physically, and the consequences of this proposition are discussed in view of the general concepts of secondary active transport and intracellular isosmotic regulation. Future studies in this area of comparative physiology should concentrate not only on the molecular characteristics of the transporter proteins, but also on the way their function is integrated in the cellular physiology of the transporting cells. J. Exp. Zool. 289:254–265, 2001. © 2001 Wiley‐Liss, Inc.

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Life with Tracers

Cited by 13 publications

References 22 publications

The Two-Membrane Model of Epithelial Transport: Koefoed-Johnsen and Ussing (1958)

The Two-Membrane Model of Epithelial Transport: Koefoed-Johnsen and Ussing (1958)

Transport of electrolytes in muscle

Transport of exogenous organic substances by invertebrate integuments: The field revisited

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