Lifespan extension in Caenorhabditis elegans by DMSO is dependent on sir-2.1 and daf-16

Wang, Xiangming; Wang, Xiaoyan; Lian-da, LI; Wang, Dan‐Qiao

doi:10.1016/j.bbrc.2010.08.113

Cited by 51 publications

(36 citation statements)

References 28 publications

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“…Animals fully recover locomotion and pumping after removal from DMSO. Moreover, Wang et al [85] found that DMSO at 0.5% and 2% concentrations significantly extended both mean and maximum lifespan in C. elegans by 25%. DMSO antagonism of insulin binding or DAF-2 signaling may explain its positive effects on lifespan in the N2 strain [85].…”

Section: Discussionmentioning

confidence: 99%

Insulin/IGF-1 Signaling, including Class II/III PI3Ks, β-Arrestin and SGK-1, Is Required in C. elegans to Maintain Pharyngeal Muscle Performance during Starvation

Dwyer

Aamodt

2013

PLoS ONE

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In C. elegans, pharyngeal pumping is regulated by the presence of bacteria. In response to food deprivation, the pumping rate rapidly declines by about 50–60%, but then recovers gradually to baseline levels on food after 24 hr. We used this system to study the role of insulin/IGF-1 signaling (IIS) in the recovery of pharyngeal pumping during starvation. Mutant strains with reduced function in the insulin/IGF-1 receptor, DAF-2, various insulins (INS-1 and INS-18), and molecules that regulate insulin release (UNC-64 and NCA-1; NCA-2) failed to recover normal pumping rates after food deprivation. Similarly, reduction or loss of function in downstream signaling molecules (e.g., ARR-1, AKT-1, and SGK-1) and effectors (e.g., CCA-1 and UNC-68) impaired pumping recovery. Pharmacological studies with kinase and metabolic inhibitors implicated class II/III phosphatidylinositol 3-kinases (PI3Ks) and glucose metabolism in the recovery response. Interestingly, both over- and under-activity in IIS was associated with poorer recovery kinetics. Taken together, the data suggest that optimum levels of IIS are required to maintain high levels of pharyngeal pumping during starvation. This work may ultimately provide insights into the connections between IIS, nutritional status and sarcopenia, a hallmark feature of aging in muscle.

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Section: Discussionmentioning

confidence: 99%

Insulin/IGF-1 Signaling, including Class II/III PI3Ks, β-Arrestin and SGK-1, Is Required in C. elegans to Maintain Pharyngeal Muscle Performance during Starvation

Dwyer

Aamodt

2013

PLoS ONE

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“…used DMSO as a solvent 24 . In light of the demonstrated effect of DMSO on the lifespan of C. elegans 45 , different experimental conditions such as solvents used may have resulted in, or contributed to, the functional differences. Together, our C. elegans data suggest that myricetin increased organismal lifespan and healthspan in a SIRT1-dependent manner.…”

Section: Discussionmentioning

confidence: 99%

Myricetin improves endurance capacity and mitochondrial density by activating SIRT1 and PGC-1α

Jung

Lee

Ryu

et al. 2017

Sci Rep

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Robust mitochondrial respiration provides energy to support physical performance and physiological well-being, whereas mitochondrial malfunction is associated with various pathologies and reduced longevity. In the current study, we tested whether myricetin, a natural flavonol with diverse biological activities, may impact mitochondrial function and longevity. The mice were orally administered myricetin (50 mg/kg/day) for 3 weeks. Myricetin significantly potentiated aerobic capacity in mice, as evidenced by their increased running time and distance. The elevated mitochondrial function was associated with induction of genes for oxidative phosphorylation and mitochondrial biogenesis in metabolically active tissues. Importantly, myricetin treatment led to decreased PGC-1α acetylation through SIRT1 activation. Furthermore, myricetin significantly improved the healthspan and lifespan of wild-type, but not Sir-2.1-deficient, C. elegans. These results demonstrate that myricetin enhances mitochondrial activity, possibly by activating PGC-1α and SIRT1, to improve physical endurance, strongly suggesting myricetin as a mitochondria-activating agent.

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“…Interestingly, another solvent molecule, DMSO, used in many aging studies as a control, was recently shown to result in C. elegans adult lifespan extension in a sir-2.1 and daf-16 dependent manner [42].…”

Section: Discussionmentioning

confidence: 99%

Caenorhabditis elegans Battling Starvation Stress: Low Levels of Ethanol Prolong Lifespan in L1 Larvae

et al. 2012

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The nematode Caenorhabditis elegans arrests development at the first larval stage if food is not present upon hatching. Larvae in this stage provide an excellent model for studying stress responses during development. We found that supplementing starved larvae with ethanol markedly extends their lifespan within this L1 diapause. The effects of ethanol-induced lifespan extension can be observed when the ethanol is added to the medium at any time between 0 and 10 days after hatching. The lowest ethanol concentration that extended lifespan was 1 mM (0.005%); higher concentrations to 68 mM (0.4%) did not result in increased survival. In spite of their extended survival, larvae did not progress to the L2 stage. Supplementing starved cultures with n-propanol and n-butanol also extended lifespan, but methanol and isopropanol had no measurable effect. Mass spectrometry analysis of nematode fatty acids and amino acids revealed that L1 larvae can incorporate atoms from ethanol into both types of molecules. Based on these data, we suggest that ethanol supplementation may extend the lifespan of L1 larvae by either serving as a carbon and energy source and/or by inducing a stress response.

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Lifespan extension in Caenorhabditis elegans by DMSO is dependent on sir-2.1 and daf-16

Cited by 51 publications

References 28 publications

Insulin/IGF-1 Signaling, including Class II/III PI3Ks, β-Arrestin and SGK-1, Is Required in C. elegans to Maintain Pharyngeal Muscle Performance during Starvation

Insulin/IGF-1 Signaling, including Class II/III PI3Ks, β-Arrestin and SGK-1, Is Required in C. elegans to Maintain Pharyngeal Muscle Performance during Starvation

Myricetin improves endurance capacity and mitochondrial density by activating SIRT1 and PGC-1α

Caenorhabditis elegans Battling Starvation Stress: Low Levels of Ethanol Prolong Lifespan in L1 Larvae

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