Lifestyle and Horizontal Gene Transfer-Mediated Evolution of Mucispirillum schaedleri, a Core Member of the Murine Gut Microbiota

Loy, Alexander; Pfann, Carina; Steinberger, Michaela; Hanson, Buck; Herp, Simone; Brugiroux, Sandrine; Gomes-Neto, João Carlos; Boekschoten, Mark V.; Schwab, Clarissa; Urich, Tim; Ramer‐Tait, Amanda E.; Rattei, Thomas; Stecher, Bärbel; Berry, David

doi:10.1128/msystems.00171-16

Cited by 165 publications

(147 citation statements)

References 98 publications

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“…In our study, ALA and LA supplementation sex‐dependently modulated gut microbiota, which might drive the inflammatory response. ALA administration reversed a series of inflammation‐associated species from Paraprevotella , Parabacteroides , Clostridium XIVa, Bilophila , Mucispirillum schaedleri and Olsenella , indicating its anti‐inflammatory and fat‐reducing effect in DIO male mice. In addition, enriched Butyricicoccus and Lachnospiracea incertae sedis augmented the fermentation of starches to produce SCFAs, which are considered as signaling molecules benefiting the energy metabolism .…”

Section: Discussionmentioning

confidence: 94%

“…As opposed to HFD‐fed mice, HFD‐ALA male mice have less abundant species Mucispirillum schaedleri and other bacterial species from genera Paraprevotella , Parabacteroides , Clostridium XIVa and Bilophila , which have been positively associated with inflammation, and more abundant species from Olsenella , which was initially reduced by HFD and negatively correlated with obesity, Butyricicoccus and Lachnospiracea incertae sedis involved in short‐chain fatty acids (SCFAs) production (Figure D). Additionally, we observed enriched genera Lactobacillus which was acknowledged as probiotics to improve intestinal function.…”

Section: Resultsmentioning

confidence: 99%

“…In addition, LA‐fed female mice had more Enterococcus faecalis , which was reported to attenuate pro‐inflammatory cytokine secretions through JNK and p38 signaling pathways. Moreover, decreased abundance of Desulfovibrio , which converted sulfate to sulfide, and less abundant Mucispirillum schaedleri may also contribute to relieved inflammation.…”

Section: Discussionmentioning

confidence: 99%

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Essential Fatty Acids Linoleic Acid and α‐Linolenic Acid Sex‐Dependently Regulate Glucose Homeostasis in Obesity

Zhuang

Shou

Wang

et al. 2018

Molecular Nutrition Food Res

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Section: Discussionmentioning

confidence: 94%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Essential Fatty Acids Linoleic Acid and α‐Linolenic Acid Sex‐Dependently Regulate Glucose Homeostasis in Obesity

Zhuang

Shou

Wang

et al. 2018

Molecular Nutrition Food Res

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“…Pigs fed RPS had significantly increased levels of Terrisporobacter, Sarcina , and Clostridium sensu stricto 1 compared to CON-fed pigs. CON-fed pigs had a significant enrichment of Mucispirillum , as well as occasional enrichment of various Proteobacteria genera such as Helicobacter, Sutterella , and Campylobacter in different tissues, and many of the genera enriched in CON group harbored members known to utilize respiratory metabolisms (13–16). One 16S rRNA gene OTU, OTU00087, was largely responsible for the differential abundance of the genus Clostriduim sensu stricto 1 between the two treatment groups.…”

Section: Resultsmentioning

confidence: 99%

Resistant potato starch fuels beneficial host-microbe interactions in the gut

Trachsel¹,

Briggs²,

Gabler³

et al. 2018

Preprint

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20Interactions between diet, the microbiota, and the host set the ecological conditions in the gut 21 and have broad implications for health. Prebiotics are dietary compounds that may shift these 22 conditions towards health by promoting the growth of beneficial microbes. Pigs fed a diet 23 amended with 5% resistant potato starch (RPS) exhibited alterations associated with gut health 24 relative to swine fed an unamended diet (CON). RPS intake increased abundances of anaerobic 25 Clostridia in feces and several tissues, as well as intestinal concentrations of butyrate. 26 Functional gene amplicons suggested bacteria similar to Anaerostipes hadrus were stimulated by 27 RPS intake. The CON treatment exhibited increased abundances of several genera of 28 Proteobacteria (which utilize respiratory metabolisms) in several location. RPS intake increased 29 the abundance of regulatory T cells in the cecum, but not periphery, and cecal immune status 30 alterations were indicative of enhanced mucosal defenses. A network analysis of host and 31 microbial changes in the cecum revealed that regulatory T cells positively correlated with 32 butyrate concentration, luminal IgA concentration, expression of IL-6 and DEF1B, and several 33 mucosa-associated bacterial taxa. Thus, the administration of RPS modulated the microbiota and 34 host response, improved cecal barrier function, promoted immunological tolerance, and reduced 35 the niche for bacterial respiration.36 Importance 37The gut microbiota is central to host health. Many disease states and disorders appear to 38 arise from interactions between the gut microbial community and host immune system. As a 39 result, methods and interventions to support the growth and activity of beneficial gut microbes 40 are being actively pursued. Feeding the gut microbiota with dietary compounds, known as 41 prebiotics, is one of the most promising ways to support gut health. Here we describe a 42 successful prebiotic intervention in weaning swine, a relevant translational model for human 43 health. This work unites microbial and immunological data and demonstrates one way a 44 prebiotic intervention can play out for the benefit of the host. 45 46 Introduction 47 Dietary prebiotics, such as resistant starches, provide an attractive alternative to 48 antibiotics for improved animal health, including humans (1). Resistant starches are compounds 49 that are not digested by the host in the small intestine, thereby arriving in the large intestine as 50 microbial-accessible carbohydrates. Bacteria that consume resistant starches are normal members 51 of the microbiota and are associated with intestinal health (2). Without access to diet-derived 52 carbohydrates, bacteria will harvest host-derived sugars from the intestinal mucus layer, 53 compromising barrier function (3). However, if dietary carbohydrates are accessible, bacteria 54 will ferment these compounds and release beneficial metabolites, particularly short-chain fatty 55 acids (SCFAs). Host cells consume the vast majority of ...

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“…One recent example is the isolation and formal description of Mucispirillum schaedleri ATCC BAA-1009 T , a close relative of ASF 457 (13). The type strain has undergone additional characterizations that provide insights into the lifestyle of this species in the mucus layer of the mouse intestinal tract (14), which may also have implications for interpreting the role of ASF 457 in ASF and the use of ASF in general. For other strains, such as ASF 502, less information is available.…”

Section: Introductionmentioning

confidence: 99%

Description of Schaedlerella arabinophila gen. nov., sp. nov., a D-arabinose utilizing bacterium isolated from feces of C57BL/6J mice and a close relative of Clostridium sp. ASF 502

Miyake

et al. 2018

Preprint

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Description of Schaedlerella arabinophila gen. nov., sp. nov., a D-arabinose utilizing 1 bacterium isolated from feces of C57BL/6J mice and a close relative of Clostridium sp. ASF 2 502 3 Abstract 21 The use of gnotobiotics has gained large interest in recent years due to technological advances 22 that have revealed the importance of host-associated microbiomes for host physiology and 23 health. One of the oldest and most important gnotobiotics mouse model, the Altered Schaedler 24 Flora (ASF) has been used for several decades. ASF comprises eight different bacterial species, 25 which have been characterized to different extent, but only few are available through public 26 strain collections. Here, the isolation of a close relative to one of the less studied ASF strains, 27 Clostridium sp. ASF 502, is reported. Isolate TLL-A1, which shares 99.6% 16S rRNA gene 28 sequence identity with Clostridium sp. ASF 502, was obtained from feces of C57BL/6J mice 29 where is was detectable at a relative abundance of less than one percent. D-arabinose was used 30 as sole carbon source in the anaerobic cultivation medium. Growth experiments with TLL-A1 31 on different carbon sources and analysis of its ~6.5 gigabase genome indicate that TLL-A1 32 harbors a large gene repertoire to utilize different carbohydrates for growth. Comparative 33 genome analyses of TLL-A1 and Clostridium sp. ASF 502 reveal differences in genome 34 content between the two strains, in particular with regards to carbohydrate activating enzymes. 35Based on physiology and genomic analysis it is proposed to name TLL-A1 to gen. nov. sp. nov 36 Schaedlerella arabinophila TLL-A1 (DSMZ 106076 T ; KCTC 15657 T ). The closely related 37 Clostridium sp. ASF 502 is proposed to be renamed to Schaedlerella arabinophila to reflect its 38 taxonomic standing and to keep 'ASF 502' as strain designation. 39 40 41 42 43 Importance 44The Altered Schaedler Flora (ASF) remains an important tool to mechanistically investigate 45 host-microbe interactions in the mammalian alimentary tract. Extensively characterizing the 46 eight different bacterial strains, which are constituting ASF, has the potential to further increase 47 the definition of this widely used model microbial community and to enhance our 48 understanding of how individual microorganism interact with a host and/or how they may 49 affect its physiology. However, some of the ASF strains have unfortunately been lost or are not 50 easily accessible to the scientific community. The isolation and characterization of the here 51 described species, proposed to be named Schaedlerella arabinophila, which is closely related 52 to Clostridium species ASF502, may therefore be an important corner stone in further 53 improving the value of studies using ASF or other defined synthetic microbial communities 54 that require usage of autochthonous microorganisms in gnotobiotic mice. 55 56 57 58 59 60 61 62 63 64 65Microbiome studies have revealed extensive insights into the complex associations of 66 microorganisms ...

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Lifestyle and Horizontal Gene Transfer-Mediated Evolution of Mucispirillum schaedleri, a Core Member of the Murine Gut Microbiota

Cited by 165 publications

References 98 publications

Essential Fatty Acids Linoleic Acid and α‐Linolenic Acid Sex‐Dependently Regulate Glucose Homeostasis in Obesity

Essential Fatty Acids Linoleic Acid and α‐Linolenic Acid Sex‐Dependently Regulate Glucose Homeostasis in Obesity

Resistant potato starch fuels beneficial host-microbe interactions in the gut

Description of Schaedlerella arabinophila gen. nov., sp. nov., a D-arabinose utilizing bacterium isolated from feces of C57BL/6J mice and a close relative of Clostridium sp. ASF 502

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