2009
DOI: 10.1016/j.bbrc.2009.08.098
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Ligand binding analyses of the putative peptide transporter YjdL from E. coli display a significant selectivity towards dipeptides

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Cited by 38 publications
(52 citation statements)
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“…A previously documented substrate of several bacterial POTs, β-Ala-Lys-N-7-amino-4 methylcoumarin-3-acetic acid (β-Ala-Lys-AMCA) was used as a reporter substrate and was also tested to measure the specific uptake of the substrate by NmPOT ( fig. 1 a; K t = 3.5 ± 0.6) [Ernst et al, 2009;Jensen et al, 2012a;Weitz et al, 2007]. We subsequently tested the inhibitory potencies of mono-, di-, tri-, and tetra-alanine, respectively ( table 1 ), which showed a prototypical preference as also observed for mammalian POTs [Daniel et al, 2006] for both diand trialanine with no or little apparent affinity for alanine or tetra-alanine.…”
Section: Resultsmentioning
confidence: 99%
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“…A previously documented substrate of several bacterial POTs, β-Ala-Lys-N-7-amino-4 methylcoumarin-3-acetic acid (β-Ala-Lys-AMCA) was used as a reporter substrate and was also tested to measure the specific uptake of the substrate by NmPOT ( fig. 1 a; K t = 3.5 ± 0.6) [Ernst et al, 2009;Jensen et al, 2012a;Weitz et al, 2007]. We subsequently tested the inhibitory potencies of mono-, di-, tri-, and tetra-alanine, respectively ( table 1 ), which showed a prototypical preference as also observed for mammalian POTs [Daniel et al, 2006] for both diand trialanine with no or little apparent affinity for alanine or tetra-alanine.…”
Section: Resultsmentioning
confidence: 99%
“…In order to characterize NmPOT, we expressed it in E. coli using the pTTQ-18 vector, which has been used for overexpression of a number of bacterial POTs [Ernst et al, 2009;Prabhala et al, 2014b]. NmPOT was expressed Ala >10 >11.14 >10 >10.14 Ala-Ala 0.39 ± 0.04 2 1.52 ± 0.04 0.14 ± 0.02 0.12 ± 0. with a hexahistidine tag enabling detection by Western blotting ( fig.…”
Section: Resultsmentioning
confidence: 99%
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“…This uptake of di-and tripeptides provides cells with amino acids that act as building blocks for protein biosynthesis but also as sources of nitrogen, sulfur, and carbon for the synthesis of various metabolites. During the past years several PTR family transporters have been functionally well characterized (3)(4)(5)(6)(7)(8)(9)(10)(11)(12). All these diand tripeptide transporters share unique specificities (2).…”
mentioning
confidence: 99%
“…The crystal structures of bacterial peptide transporters from Shewanella oneidensis (PepT So and PepT So2 ), Streptococcus thermophilus (PepT St ), and Geobacillus kaustophilus (GkPOT) revealed 14 TMHs (10,(20)(21)(22). In Escherichia coli, four PTR family members have been characterized: dipeptide and tripeptide permease A (DtpA, formerly named YdgR or TppB) (3,4,23), DtpB (formerly YhiP) (3), DtpC (formerly YjdL) (11,(24)(25)(26), and DtpD (formerly YbgH) (27). Among these transporters, DtpA shows peptide selectivity very similar to that of hPEPT1 (3,4).…”
mentioning
confidence: 99%