Ligand binding and signalling pathways of PTH receptors in sea bream (Sparus auratus) enterocytes

Rotllant, Josep; Guerreiro, Marta; Redruello, Begoña; Fernandes, Helena; Apolonia, Luis; Anjos, Liliana; Canário, Adelino V.M.; Power, Deborah M.

doi:10.1007/s00441-005-0070-7

Cited by 21 publications

(20 citation statements)

References 36 publications

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“…§P Ͻ 0.05, significant difference between controls and PTHrPtreated preparations (1-way ANOVA). transport and is consistent with the recent demonstration of a PTHrP-activated PTH3R receptor in sea bream enterocytes (34). These results imply that PTHrP plays an important role in fish calcium homeostasis by increasing net calcium absorption and making more calcium available for physiological requirements, such as deposition into bone and scales.…”

Section: Discussionsupporting

confidence: 91%

“…The ion-transporting mechanisms underlying these effects remain unknown. cAMP is known to alter intestinal permeability (9), and PTHrP stimulates the release of cAMP in sea bream enterocytes (34). However, paracellular permeability in the intestine can also be altered by other factors such as phospholipase C, tyrosine kinases, calcium, protein kinase C, and the heterotrimeric G proteins (41).…”

Section: Discussionmentioning

confidence: 99%

“…One possibility is that more than one receptor could be involved in the different regions. At present, there is only evidence for a single PTH receptor (PTH3R) in sea bream duodenum (34), but the hindgut and rectum regions have not been specifically analyzed for the presence of PTH receptors. In addition, or alternatively, the active signaling pathways present along the intestine may be different, triggering a variety of ion transport mechanisms in response to PTHrP.…”

Section: Discussionmentioning

confidence: 99%

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Parathyroid hormone-related protein regulates intestinal calcium transport in sea bream (Sparus auratus)

Fuentes¹,

Figueiredo²,

Power³

et al. 2006

American Journal of Physiology-Regulatory, Integrative and Comp

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-Parathyroid hormone-related protein (PTHrP) is a factor associated with normal development and physiology of the nervous, cardiovascular, immune, reproductive, and musculoskeletal systems in higher vertebrates. It also stimulates whole body calcium uptake in sea bream (Sparus auratus) larvae with an estimated 60% coming from intestinal uptake in seawater. The present study investigated the role of PTHrP in the intestinal calcium transport in the sea bream in vitro. Unidirectional mucosal-to-serosal and serosal-to-mucosal 45 Ca fluxes were measured in vitro in duodenum, hindgut, and rectum mounted in Ussing chambers. In symmetric conditions with the same saline, bathing apical and basolateral sides of the preparation addition of piscine PTHrP 1-34 (6 nM) to the serosal surface resulted in an increase in mucosal to serosal calcium fluxes in duodenum and hindgut and a reduction in serosal to mucosal in the rectum, indicating that different mechanisms are responsive to PTHrP along the intestine. In control asymmetric conditions, with serosal normal and mucosal bathed with a saline similar in composition to the intestinal fluid, there was a net increase in calcium uptake in all regions. The addition of 6 nM PTHrP 1-34 increased net calcium uptake two-to threefold in all regions. The stimulatory effect of PTHrP on net intestinal calcium absorption is consistent with a hypercalcemic role for the hormone. The results support the view that PTHrP, alone or in conjunction with recently identified PTH-like peptides, counteracts in vivo the hypocalcemic effects of stanniocalcin. fish; intestine; hypercalcemia IN HIGHER VERTEBRATES, calcium homeostasis is controlled largely by the interplay of the hypocalcemic hormone calcitonin, produced in the C cells of the thyroid gland, and the hypercalcemic parathyroid hormone (PTH), produced in the parathyroid gland. The parathyroid gland first appears as an isolated and functional gland in amphibians (6), and its formation is controlled by the transcription factor Gcm-2. In fish, Gcm-2 controls internal gill bud formation (18,28), and its coexpression with PTH transcripts has led to the suggestion that the parathyroid gland derived from the fish gill (28). However, coexpression of PTH and Gcm-2 has not been confirmed (17).Early studies (29) suggested that the pituitary gland of fishes contains a hypercalcemic factor related to, but distinct from, PTH. Furthermore, immunohistochemical studies indicated the presence in the pituitary and other tissues of several teleosts of parathyroid hormone-related protein (PTHrP) (7,20), a factor that shares with PTH hypercalcemic activity and a common receptor (25). The first fish PTHrP gene and cDNA were cloned in the puffer fish, Takifugu rubripes (31), and in the sea bream, Sparus

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Parathyroid hormone-related protein regulates intestinal calcium transport in sea bream (Sparus auratus)

Fuentes¹,

Figueiredo²,

Power³

et al. 2006

American Journal of Physiology-Regulatory, Integrative and Comp

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“…and gonad, followed by skin, brain, and pituitary (53). PTH1R also is present in scales (111,116), whereas PTH3R has been detected in enterocytes (115), and both receptors are expressed in the interrenal glands (114). The PTH2R was initially isolated from catfish Ictalurus punctatus liver and kidney and from the zebrafish kidney (118).…”

Section: Pth and Pthrp Receptorsmentioning

confidence: 98%

The parathyroid hormone family of peptides: structure, tissue distribution, regulation, and potential functional roles in calcium and phosphate balance in fish

Guerreiro¹,

Renfro²,

Power³

et al. 2007

American Journal of Physiology-Regulatory, Integrative and Comp

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Parathyroid hormone (PTH) and PTH-related protein (PTHrP) are two factors that share amino acid sequence homology and act via a common receptor. In tetrapods, PTH is the main endocrine factor acting in bone and kidney to regulate calcium and phosphate. PTHrP is an essential paracrine developmental factor present in many tissues and is involved in the regulation of ossification, mammary gland development, muscle relaxation, and other functions. Fish apparently lack an equivalent of the parathyroid gland and were long thought to be devoid of PTH. Only in recent years has the existence of PTH-like peptides and their receptors in fish been firmly established. Two forms of PTH, two of PTHrP, and a protein with intermediate characteristics designated PTH-L are encoded by separate genes in teleost fish. Three receptors encoded by separate genes in fish mediate PTH/PTHrP actions, whereas only two receptors have so far been found in terrestrial vertebrates. PTHrP has been more intensively studied than PTH, from lampreys to advanced teleosts. It is expressed in many tissues and is present in high concentration in fish blood. Administration of this peptide alters calcium metabolism and has marked effects on associated gene expression and enzyme activity in vivo and in vitro. This review provides a comprehensive overview of the physiological roles, distribution, and molecular relationships of the piscine PTH-like peptides.

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“…PTHrP can upregulate CYP27A1 mRNA levels, which suggests a similar role for PTHrP in fish as that of PTH (and PTHrP) in mammalian vitamin D metabolism. Indeed, consensus exists that PTHrP and PTH share common receptors in fish and mammals alike and thus such a finding for PTHrP in fish is not surprising as the PTHrP receptor is expressed in sea bream kidney and many other tissues , Rotllant et al 2006). …”

Section: Discussionmentioning

confidence: 99%

CYP27A1 expression in gilthead sea bream (Sparus auratus, L.): effects of calcitriol and parathyroid hormone-related protein

Bevelander

Pinto

Canário

et al. 2007

Journal of Endocrinology

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Little is known about vitamin D metabolism in fishes. Several reports have shown hydroxylase activities in various organs to produce vitamin D metabolites, but the enzymes involved have not been isolated or characterized. We isolated and characterized a renal mitochondrial hydroxylase, CYP27A1, that governs vitamin D metabolism in gilthead sea bream, Sparus auratus. The enzyme is highly expressed in kidney and to a far lesser extent in liver. When treated with 25-hydroxy vitamin D or calcitriol, the kidney responded differentially and time dependently with CYP27A1 mRNA expression levels. This response substantiates a role for CYP27A1 in fish vitamin D metabolism. This notion is strengthened by upregulation of CYP27A1 in sea bream treated with parathyroid hormone-related protein (PTHrP), and suggests an original role for PTHrP in calcitriol-regulated processes n fish similar to the role of PTH in mammalian vitamin D-dependent processes.

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Ligand binding and signalling pathways of PTH receptors in sea bream (Sparus auratus) enterocytes

Cited by 21 publications

References 36 publications

Parathyroid hormone-related protein regulates intestinal calcium transport in sea bream (Sparus auratus)

Parathyroid hormone-related protein regulates intestinal calcium transport in sea bream (Sparus auratus)

The parathyroid hormone family of peptides: structure, tissue distribution, regulation, and potential functional roles in calcium and phosphate balance in fish

CYP27A1 expression in gilthead sea bream (Sparus auratus, L.): effects of calcitriol and parathyroid hormone-related protein

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