Ligand binding to six recombinant pheromone-binding proteins of Antheraea polyphemus and Antheraea pernyi

Maida, Rosario; Ziegelberger, Gunde; Kaissling, Karl-Ernst

doi:10.1007/s00360-003-0366-4

Cited by 81 publications

(64 citation statements)

References 33 publications

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“…One possibility is that ABPX or GOBP1 would play this role, although their labelling was hardly above background, and given the low amino-acid identity of these proteins with PBP, this appears even more improbable. Alternatively, a second PBP might be present in the long s. trichodea of B. mori, similar to the situation in A. polyphemus, where three PBPs have been identified in male antenna (Maida et al, 2000(Maida et al, , 2003. Indeed, even though so far only a single PBP has been characterised in B. mori (Maida et al, 1993;Krieger et al, 1996), there are indications that minute amounts of additional PBPs may be present (Maida et al, 1997).…”

Section: Obp Functionmentioning

confidence: 83%

The expression pattern of four odorant-binding proteins in male and female silk moths, Bombyx mori

Maida¹,

Mameli

Müller³

et al. 2005

J Neurocytol

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Four recombinant odorant-binding proteins of Bombyx mori, pheromone-binding protein (PBP), general odorant-binding protein 1 (GOBP1), general odorant-binding protein 2 (GOBP2) and antennal binding protein X (ABPX), were expressed in E. coli and used to raise polyclonal antisera. Immunoblots of antennal homogenates showed that these antisera were specific. In Western blot analysis and immunocytochemical labelling experiments, the sera against recombinant PBP and GOBP2 of B. mori gave identical results as sera against native PBP and GOBP2 of Antheraea polyphemus, respectively, thus confirming earlier results obtained with the latter. Labelling consecutive cross sections of various sensillum types with all four antisera revealed different labelling patterns in male and female sensilla (s.) trichodea and s. basiconica. Long s. trichodea in males and females represented uniform labelling types, whereas for short s. trichodea, s. intermedia, and s. basiconica a great variety of labelling patterns was observed, some being more common than others. Long s. trichodea, which in males are uniformly tuned to the pheromone components bombykol and bombykal, all strongly expressed PBP; labelling with antisera against the other three odorant-binding proteins hardly was above background, only in some hairs GOBP1 was expressed somewhat more strongly. Long s. trichodea of females, which respond specifically to linalool and benzoic acid, showed a different labelling pattern. Here, we observed strong labelling with antibodies against GOBP2 and medium labelling with anti-GOBP1, sometimes with anti-ABPX. S. basiconica in both sexes most commonly co-expressed GOBP1 and GOBP2, but other patterns were occasionally found, with some of them showing PBP expression, also in females. The great variety of labelling types in short s. trichodea, s. intermedia, and s. basiconica suggests a similar variety of functional subtypes as observed in plant odour-sensitive sensilla of other moth species.

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Section: Obp Functionmentioning

confidence: 83%

The expression pattern of four odorant-binding proteins in male and female silk moths, Bombyx mori

Maida¹,

Mameli

Müller³

et al. 2005

J Neurocytol

Self Cite

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“…In addition, OBPs might be involved in establishing the olfactory code (Van den Berg and Ziegelberger, 1991;Maida et al, 2003;Pophof, 2004;Matsuo et al, 2007;Laughlin et al, 2008) as well as in stimulus inactivation (Pelosi and Maida, 1995;Ziegelberger, 1995;Kaissling, 2001 . CSPs are more conserved, with a specific motif of four cysteines that form two disulphide bridges between neighbouring residues.…”

Section: Insect Chemosensory Gene Family Evolutionmentioning

confidence: 99%

Molecular evolution of the major chemosensory gene families in insects

2009

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Chemoreception is a crucial biological process that is essential for the survival of animals. In insects, olfaction allows the organism to recognise volatile cues that allow the detection of food, predators and mates, whereas the sense of taste commonly allows the discrimination of soluble stimulants that elicit feeding behaviours and can also initiate innate sexual and reproductive responses. The most important proteins involved in the recognition of chemical cues comprise moderately sized multigene families. These families include odorant-binding proteins (OBPs) and chemosensory proteins (CSPs), which are involved in peripheral olfactory processing, and the chemoreceptor superfamily formed by the olfactory receptor (OR) and gustatory receptor (GR) families. Here, we review some recent evolutionary genomic studies of chemosensory gene families using the data from fully sequenced insect genomes, especially from the 12 newly available Drosophila genomes. Overall, the results clearly support the birth-and-death model as the major mechanism of evolution in these gene families. Namely, new members arise by tandem gene duplication, progressively diverge in sequence and function, and can eventually be lost from the genome by a deletion or pseudogenisation event. Adaptive changes fostered by environmental shifts are also observed in the evolution of chemosensory families in insects and likely involve reproductive, ecological or behavioural traits. Consequently, the current size of these gene families is mainly a result of random gene gain and loss events. This dynamic process may represent a major source of genetic variation, providing opportunities for FUTURE specific adaptations.

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“…The identification of multiple subtypes within one species (Krieger et al 1991;Maida et al 2000;Abraham et al 2005) has led to the concept that a distinct PBP-subtype may be tuned to bind and transport only a defined pheromone component. Indeed, recent work indicated that a PBP-subtype interacts differentially with various pheromone components and displays distinct binding specificities for different pheromone components (Feixas et al 1995;Plettner et al 2000;Mohl et al 2002;Bette et al 2002;Maida et al 2003). In the silkmoth, Bombyx mori, only one PBP (BmorPBP) has been identified to date (Krieger et al 1996); a finding which appeared to coincide with the apparently very limited complexity of the sex-pheromone blend (bombykol, bombykal).…”

Section: Introductionmentioning

confidence: 96%

Candidate pheromone binding proteins of the silkmoth Bombyx mori

et al. 2006

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Pheromone reception is thought to be mediated by pheromone binding proteins (PBPs) in the aqueous lymph of the antennal sensilla. Recent studies have shown that the only known PBP of Bombyx mori (BmorPBP1) appears to be specifically tuned to bombykol but not to bombykal, raising the question of whether additional subtypes may exist. We have identified two novel genes, which encode candidate PBPs (BmorPBP2, BmorPBP3). Comparison with PBPs from various moth species have revealed a high degree of sequence identity and the three BmorPBP-subtypes can be assigned to distinct groups within the moth PBP family. In situ hybridization revealed that BmorPBP2 and BmorPBP3 are expressed only in relatively few cells compared to the number of cells expressing BmorPBP1. Double-labeling experiments have shown that the two novel BmorPBPs are expressed in the same cells but are not co-expressed with BmorPBP1. Furthermore, unlike BmorPBP1, cells expressing the newly identified PBPs did not surround neurons containing the BmOR-1 receptor. The results indicate that BmorPBP2 and BmorPBP3 are located in sensilla types, which are different from the long sensilla trichodea.

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Ligand binding to six recombinant pheromone-binding proteins of Antheraea polyphemus and Antheraea pernyi

Cited by 81 publications

References 33 publications

The expression pattern of four odorant-binding proteins in male and female silk moths, Bombyx mori

The expression pattern of four odorant-binding proteins in male and female silk moths, Bombyx mori

Molecular evolution of the major chemosensory gene families in insects

Candidate pheromone binding proteins of the silkmoth Bombyx mori

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