2023
DOI: 10.1128/mbio.02143-23
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Ligand-displaying Escherichia coli cells and minicells for programmable delivery of toxic payloads via type IV secretion systems

Yang Grace Li,
Kouhei Kishida,
Natsumi Ogawa-Kishida
et al.

Abstract: Bacterial type IV secretion systems (T4SSs) are highly versatile macromolecular translocators and offer great potential for deployment as delivery systems for therapeutic intervention. One major T4SS subfamily, the conjugation machines, are well-adapted for delivery of DNA cargoes of interest to other bacteria or eukaryotic cells but generally exhibit modest transfer frequencies and lack specificity for target cells. Here, we tested the efficacy of a surface-displayed nanobody/antigen (Nb/Ag) pairing system to… Show more

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Cited by 7 publications
(8 citation statements)
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“…Thus, while TraN ED and TraN F are swappable between the pED208 and F systems, in fact, they mediate formation of stable mating pairs through distinct ligand - receptor interactions. TraN subunits also possess large, Cys-rich periplasmic regions that contribute in unspecified ways to DNA transfer and pilus production [25, 64]. Intriguingly, no TraN interactions with other T4SS components have yet been identified [54, 56], leaving open the question of how TraN F and TraN ED can function interchangeably in the pED208 and F systems.…”
Section: Resultsmentioning
confidence: 99%
“…Thus, while TraN ED and TraN F are swappable between the pED208 and F systems, in fact, they mediate formation of stable mating pairs through distinct ligand - receptor interactions. TraN subunits also possess large, Cys-rich periplasmic regions that contribute in unspecified ways to DNA transfer and pilus production [25, 64]. Intriguingly, no TraN interactions with other T4SS components have yet been identified [54, 56], leaving open the question of how TraN F and TraN ED can function interchangeably in the pED208 and F systems.…”
Section: Resultsmentioning
confidence: 99%
“…The nanobodies isolated in this study exhibit stable and specific interactions with their cognate target antigens, underscoring their potential as versatile tools for engineering bacteria-bacteria adhesions. These CAMs present opportunities for future applications, such as engineering complex multi-component metabolic pathways and biomaterials [5][6][7] , as well as precision-targeted manipulation 8,10 . Furthermore, we anticipate our method will prove compatible with existing cell-cell adhesion platforms, enabling the design and fine-tuning of multicellular assemblies, patterns, and morphologies, thereby positioning CAMs as key components in the synthetic microbiology toolkit 11,12 .…”
Section: Discussionmentioning
confidence: 99%
“…Overall, our results herein highlight the vast potential of our screening platform and its potential integration with other synthetic biology tools. For instance, we envisage additional possibilities using CRISPR-based systems or engineered antimicrobials 10,[31][32][33] . Combining different synthetic biology approaches for the precise manipulation of microbial communities could yield synergistic benefits with diverse applications.…”
Section: Discussionmentioning
confidence: 99%
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