Ligand-Modulated Catalytic Selectivity of Ag Clusterzyme for Relieving Multiorgan Injury via Inhabiting Acute Oxidative Stress

Abstract

The artificial enzymes at the atomic level have shown great potential in chemical biology and nanomedicine, and modulation of catalytic selectivity is also critical to the application of nanozymes. In this work, atomic precision Ag25 clusterzymes protected by single- and dual-ligand were developed. Further, the catalytic activity and selectivity of Ag25 clusterzymes were modulated by adjusting doping elements and ligand. The Ag24Pt1 shows more prominent antioxidant activity characteristics in the dual-ligand system, while the Ag24Cu1 possesses the superoxide dismutase-like (SOD-like) activity regardless of the single- or dual-ligand system, indicating modulated catalytic selectivity. In vitro experiments showed the Ag24Pt1-D can recover radiation induced DNA damages and eliminate the excessive reactive oxygen species (ROS) generated from radiation. Subsequent in vivo radiation protection experiments reveal that Ag24Cu1-S and Ag24Pt1-D can improve the survival rate of irradiated mice from 0 to 40% and 30%, respectively. The detailed biological experiments confirm that the Ag24Cu1-S and Ag24Pt1-D can recover the SOD and 3,4-methylenedioxyamphetamine (MDA) levels via suppressing the chronic inflammation reaction. Nearly 60% of Ag24Cu1-S and Ag24Pt1-D can be excreted after a 1 day injection, and no obvious toxicological reactions were observed 30 days after injection.

Neuron devices: emerging prospects in neural interfaces and recognition

“…Second, biocompatibility is important for the development of neuron devices. An electrode can be modified with nanomaterials possessing high catalytic activity, such as nanoclusters 114,[119][120][121][122][123] , atomic level nanozymes 122,[124][125][126][127][128][129] and two-dimensional materials [130][131][132][133] , to reduce the inflammatory response of neural tissue. Third, integrated neuron devices can be developed to achieve multiple functions simultaneously [134][135][136] .…”

Multiscale Bioresponses of Metal Nanoclusters

“…[146] Ag 25 MHA 18 showed a similar increase in antioxidant ability after single-atom substitution and dual-ligand protection compared to Ag 24 Pt 1 (MHA/MPA) 18 and Ag 24 Cu 1 (MHA) 18 , indicating modulated catalytic selectivity. [147] Recently, metal NCs were reported to act as monoamine oxidase B inhibitors (MAOI), which paved the way for creating a more effective drug for Alzheimer's disease. [142] The Au NCs and enzymatic substrates exhibit competitive relationships.…”

“…[ 146 ] Ag 25 MHA 18 showed a similar increase in antioxidant ability after single‐atom substitution and dual‐ligand protection compared to Ag 24 Pt 1 (MHA/MPA) 18 and Ag 24 Cu 1 (MHA) 18 , indicating modulated catalytic selectivity. [ 147 ]…”

Trimanganese Tetroxide Nanozyme protects Cartilage against Degeneration by Reducing Oxidative Stress in Osteoarthritis

“…Fortunately, recent studies have shown that some nanomaterials that can reduce oxidation by denaturation in the cellular microenvironment act as bioenzymes to eliminate excessive intracellular ROS. [ 15 ] Nanozyme, a type of nanomaterial that possesses intrinsic enzyme‐like activity, catalyzes enzyme substrates efficiently under mild conditions and exhibits catalytic efficiency and enzymatic reaction kinetics similar to those of natural enzymes. [ 16 ] Compared with bioenzymes, nanozymes still maintain 85% catalytic activity even in strong acids/bases (pH ≈2–10) or over a wide temperature range (≈4–90 °C), which suggests broad clinical application prospects.…”