Ligand-Targeted Liposomes for Cancer Treatment

Sapra, Puja; Tyagi, Pradeep; Allen, Theresa M.

doi:10.2174/156720105774370159

Cited by 233 publications

(146 citation statements)

References 116 publications

(163 reference statements)

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“…Two lipids found on OsrHSA, (14:0)-lyso-PC and (14:0)-lyso-PE, were not detected at pHSA; however, both (14:0)-lyso-PC and (14:0)-lyso-PE are common phospholipids in human blood, which mainly bind to low-density lipid protein rather than HSA in vivo (28,29). Those have been applied to prepare liposomes for drug delivery or stabilization (30,31).…”

Section: Resultsmentioning

confidence: 99%

Large-scale production of functional human serum albumin from transgenic rice seeds

Ning

Xie

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

195

130

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Human serum albumin (HSA) is widely used in clinical and cell culture applications. Conventional production of HSA from human blood is limited by the availability of blood donation and the high risk of viral transmission from donors. Here, we report the production of Oryza sativa recombinant HSA (OsrHSA) from transgenic rice seeds. The level of OsrHSA reached 10.58% of the total soluble protein of the rice grain. Large-scale production of OsrHSA generated protein with a purity >99% and a productivity rate of 2.75 g/kg brown rice. Physical and biochemical characterization of OsrHSA revealed it to be equivalent to plasma-derived HSA (pHSA). The efficiency of OsrHSA in promoting cell growth and treating liver cirrhosis in rats was similar to that of pHSA. Furthermore, OsrHSA displays similar in vitro and in vivo immunogenicity as pHSA. Our results suggest that a rice seed bioreactor produces cost-effective recombinant HSA that is safe and can help to satisfy an increasing worldwide demand for human serum albumin.

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Section: Resultsmentioning

confidence: 99%

Large-scale production of functional human serum albumin from transgenic rice seeds

Ning

Xie

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

195

130

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“…Prolonged blood circulation of the liposomes is achieved with the addition of a polyethylene glycol (PEG) coating, which efficiently minimizes their removal by macrophages of the reticuloendothelial system [3][4][5][6]. Liposomes with various target-specific ligands attached to their surface are being investigated for targeted drug delivery [1,2,7]. Liposomes labeled with radioisotopes such as 99m Tc, 186 Re, 67 Ga, 111 In, and 18 F were previously employed to study the biodistribution of different types of liposomes in various animal models using scintigraphy, SPECT and PET.…”

Section: Introductionmentioning

confidence: 99%

Long-circulating liposomes radiolabeled with [18F]fluorodipalmitin ([18F]FDP)

Mařı́k

Tartis

Zhang

et al. 2007

Nuclear Medicine and Biology

101

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Synthesis of a radiolabeled diglyceride 3-[ 18 F]fluorodipalmitoyl-1,2-glycerol ( 18 F-fluorodipalmitin, [ 18 F]FDP) and its potential as a reagent for radiolabeling long-circulating liposomes were investigated. The incorporation of 18 F into the lipid molecule was accomplished by nucleophilic substitution of p-toluenesolfonyl moiety with a decay corrected yield of 43 ± 10% (n = 12). Radiolabeled long-circulating PEG-coated liposomes were prepared using a mixture of DPPC, cholesterol, DSPE-PEG2000 (61:30:9) and [ 18 F]FDP with a decay corrected yield of 70 ± 8% (n = 4). PET imaging and biodistribution studies were performed with free [ 18 Liposomes are vesicles composed of one or more concentric phospholipid bi-layers and such vesicles have been widely investigated as possible drug carriers [1,2]. Prolonged blood circulation of the liposomes is achieved with the addition of a polyethylene glycol (PEG) coating, which efficiently minimizes their removal by macrophages of the reticuloendothelial system [3][4][5][6]. Liposomes with various target-specific ligands attached to their surface are being investigated for targeted drug delivery [1,2,7]. Liposomes labeled with radioisotopes such as 99m Tc, 186 Re, 67 Ga, 111 In, and 18 F were previously employed to study the biodistribution of different types of liposomes in various animal models using scintigraphy, SPECT and PET. Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. NIH Public Access Materials and Methods GeneralThe solvents and chemicals were purchased from Aldrich (Milwaukee, WI). The 1 H and 13 C NMR spectra were recorded using a Bruker Avance 500 spectrometer and the chemical shifts are reported relative to TMS. Analytical reversed-phase HPLC was performed using a Phenomenex Jupiter 5μ C4 300A column ( . The size distribution of liposomes was determined using a particle size analyzer Nanotrac NPA150 purchased from Microtrac Inc. (North Largo, FL). The lipid concentration was determined using a Phospholipids B kit purchased from Wako Chemicals, Inc. (Richmond, VA). Synthesis of 3-tosyl-1,2-dipalmitoyl glycerol (2)The precursor 2 was prepared according to the previously published procedure [32] from 1,2-dipalmitoyl-sn-glycerol (1) and 4-toluenesulfonyl chloride. The crude product was subsequently purified by recrystalization from hexane. 1 mL). The content of the vial was dissolved in anhydrous acetonitrile (0.35 mL) and transferred into a suspension of 2 (5.5 mg) in anhydrous acetonitrile (0.5 mL). The reaction mixture was heated to 100 ºC for 20 minutes and allowed to cool to room temperature for 5 min...

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“…Liposomes are the most studied formulation of nanoparticle for drug delivery. Liposomal formulations have shown an ability to improve the pharmacokinetics and pharmacodynamics of associated drugs [41]. …”

Section: Liposomesmentioning

confidence: 99%

Nanotechnology in Cancer Diagnosis and Treatment

El-Karim¹,

El-Zahar²,

Anwar³

2015

JPP

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Nanotechnology refers to research and technology development at the atomic, molecular, and macromolecular scale, leading to the controlled manipulation and study of structures and devices with length scales in the 1-100 nanometers range. Objects at this scale, such as "nanoparticles," take on novel properties and functions that differ markedly from those seen in the bulk scale. The small size, surface tailor ability, improved solubility, and multifunctional of nanoparticles open many new research avenues for biologists. Nanoparticles are classified into inorganic nanoparticles such as gold nanoparticles and organic nanoparticles such as carbon nanotubes and dendrimers. Different types of nanoparticals such as nanocrystals, liposomes, polymeric nanoparticles, dendrimers, quantum dots and superparamagnetic nanoparticles illustrated different examples of promising nanoparticles for cancer treatment and imaging.

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Ligand-Targeted Liposomes for Cancer Treatment

Cited by 233 publications

References 116 publications

Large-scale production of functional human serum albumin from transgenic rice seeds

Large-scale production of functional human serum albumin from transgenic rice seeds

Long-circulating liposomes radiolabeled with [18F]fluorodipalmitin ([18F]FDP)

Nanotechnology in Cancer Diagnosis and Treatment

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