Ligands of toll-like receptors 2/4 differentially alter markers of inflammation, adhesion and angiogenesis by monocytes from women with pre-eclampsia in co-culture with endothelial cells

Al-ofi, Ebtisam A.; Anumba, Dilly

doi:10.1016/j.jri.2017.05.002

Cited by 15 publications

(12 citation statements)

References 48 publications

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“…While the majority of the 19 differentially expressed proteins reported here are involved in acute phase responses, all 19 proteins have altered expression in NP. However, they were identified because their expression levels were abnormal in PE, which also involves other cellular changes including oxidative stress and endothelial dysfunction, and some of the 19 proteins (e.g Heat shock protein 27, Gelsolin, Fibrinogen) are involved in these processes [55–57]. To our knowledge, Alpha-Enolase and Pregnancy-Zone Protein have not been validated in non-proteomic studies.…”

Section: Discussionmentioning

confidence: 99%

Using proteomics to advance the search for potential biomarkers for preeclampsia: A systematic review and meta-analysis

et al. 2019

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Background Preeclampsia (PE) is a leading cause of maternal and perinatal morbidity and mortality worldwide. Although predictive multiparametric screening is being developed, it is not applicable to nulliparous women, and is not applied to low-risk women. As PE is considered a heterogenous disorder, it is unlikely that any single multiparametric screening protocol containing a small group of biomarkers could have the required accuracy to predict all PE subgroups. Given the etiology of PE is complex and not fully understood, it begs the question, whether the search for biomarkers based on the predominant view of impaired placentation involving factors predominately implicated in angiogenesis and inflammation, has been too limiting. Here we highlight the enormous potential of state-of-the-art, high-throughput proteomics, to provide a comprehensive and unbiased approach to biomarker identification. Methods and findings Our literature search identified 1336 articles; after review, 45 studies with proteomic data from PE women that were eligible for inclusion. From 710 proteins with altered abundance, we identified 13 common circulating proteins, some of which had not been previously considered as prospective biomarkers of PE. An additional search of the literature for original publications testing any of the 13 common proteins using non-proteomic techniques was also undertaken. Strikingly, 9 of these common proteins had been independently evaluated in PE studies as potential biomarkers. Conclusion This study highlights the potential of using high-throughput data sets, which are comprehensive and without bias, to identify a profile of proteins that may improve predictions of PE and understanding of its etiology. We bring to the attention of the medical and research communities that the strengths and advantages of using data from high-throughput studies for biomarker discovery would be increased dramatically, if first and second trimester samples were collected for proteomics, and if standardized guidelines for patient reporting and data collection were implemented.

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Section: Discussionmentioning

confidence: 99%

Using proteomics to advance the search for potential biomarkers for preeclampsia: A systematic review and meta-analysis

et al. 2019

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“… 68–70 Compared with healthy controls, NLRP3 gene and protein expression were increased in peripheral blood mononuclear cells in patients with DR, and IL-1β and IL-18 levels were also elevated in peripheral blood mononuclear cells and vitreous fluid in patients with DR. 71 TMAO can also promote the phosphorylation of NF-κB, which in turn induces inflammatory factors, such as IL-1β, IL-6, IL-8, TNF-α, monocyte chemotactic protein-1 (MCP-1), cyclooxygenase-2 (COX-2), and ICAM. 72 , 73 ICAM-1 increases adhesion of monocytes and induces activation of protein kinase C (PKC), and down-regulates the expression of the zonulin (Zo) gene, contributing to retinal endothelial dysfunction. 74 Recent studies have shown that NLRP3 is activated in the retina of diabetic mice and human vitreous samples mediating retinal microvascular endothelial cells damage stimulated by hyperglycemia.…”

Section: Hypothesis That Gut Microbiota Cause Drmentioning

confidence: 99%

Recent Insights into the Role of Gut Microbiota in Diabetic Retinopathy

Jiao¹,

Yu²,

Yao³

et al. 2021

JIR

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The microbiome has become a hot issue in recent years. The composition, modification, alteration, and disturbance of gut microbiota were found to influence important physiological processes, including energy metabolism and microenvironmental homeostasis, and lead to various diseases, including obesity, type 2 diabetes mellitus and chronic kidney disease. Diabetic retinopathy (DR) is a major microvascular complication of diabetes mellitus and one of the leading causes of blindness and vision impairment. The underlying mechanisms in DR pathogenesis remain limited. Recently, important insights have been made regarding possible connections between gut microbiome dysbiosis and ocular disease including DR, uveitis, glaucoma, and age-related macular degeneration, and the concept of a “microbiota–gut–retina axis” has been put forward. Hence, we reviewed current understanding of the relationship between DR and gut microbiota. We summarized potential pathophysiological mechanisms that contribute to the role of the gut microbiota on DR, including hyperglycemia, anti-diabetes drugs, microbial metabolites, and inflammatory properties. We aimed to find novel effective therapeutic options to prevent the onset and development of DR.

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“…Fibrinogen, an acute‐phase protein that is released during inflammation and displays multiple immune interactions, 315 was elevated in the circulation of preeclamptic women and increased in vitro cytokine production by preeclampsia‐derived monocytes compared to those from normotensive pregnancies 316 . Interestingly, fibrinogen treatment also reduced the levels of sFlt‐1 present in a co‐culture system of preeclampsia‐derived monocytes and endothelial cells, suggesting that fibrinogen exerts distinct and unrelated effects on the cytokine production and angiogenesis pathways as part of the pathophysiology of preeclampsia 305 …”

Section: Introductionmentioning

confidence: 98%

Cellular immune responses in the pathophysiology of preeclampsia

Miller

Motomura

Galaz

et al. 2021

Journal of Leukocyte Biology

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Preeclampsia, defined as new‐onset hypertension accompanied by proteinuria occurring at 20 weeks of gestation or later, is a leading cause of perinatal morbidity and mortality worldwide. The pathophysiology of this major multi‐systemic syndrome includes defective deep placentation, oxidative stress, endothelial dysfunction, the presence of an anti‐angiogenic state, and intravascular inflammation, among others. In this review, we provide a comprehensive overview of the cellular immune responses involved in the pathogenesis of preeclampsia. Specifically, we summarize the role of innate and adaptive immune cells in the maternal circulation, reproductive tissues, and at the maternal‐fetal interface of women affected by this pregnancy complication. The major cellular subsets involved in the pathogenesis of preeclampsia are regulatory T cells, effector T cells, NK cells, monocytes, macrophages, and neutrophils. We also summarize the literature on those immune cells that have been less characterized in this clinical condition, such as γδ T cells, invariant natural killer T cells, dendritic cells, mast cells, and B cells. Moreover, we discuss in vivo studies utilizing a variety of animal models of preeclampsia to further support the role of immune cells in this disease. Finally, we highlight the existing gaps in knowledge of the immunobiology of preeclampsia that require further investigation. The goal of this review is to promote translational research leading to clinically relevant strategies that can improve adverse perinatal outcomes resulting from the obstetrical syndrome of preeclampsia.

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Ligands of toll-like receptors 2/4 differentially alter markers of inflammation, adhesion and angiogenesis by monocytes from women with pre-eclampsia in co-culture with endothelial cells

Cited by 15 publications

References 48 publications

Using proteomics to advance the search for potential biomarkers for preeclampsia: A systematic review and meta-analysis

Using proteomics to advance the search for potential biomarkers for preeclampsia: A systematic review and meta-analysis

Recent Insights into the Role of Gut Microbiota in Diabetic Retinopathy

Cellular immune responses in the pathophysiology of preeclampsia

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