Ligation of multiple protein domains using orthogonal inteins with non‐native splice junctions
Alejandro Romero‐Casañas,
Andrea García‐Lizarribar,
Jessica Castro
et al.
Abstract:Protein splicing is a self‐catalyzed process in which an internal protein domain (the intein) is excised from its flanking sequences, linking them together with a canonical peptide bond. Trans‐inteins are separated in two different precursor polypeptide chains that must assemble to catalytically self‐excise and ligate the corresponding flanking exteins to join even when expressed separately either in vitro or in vivo. They are very interesting to construct full proteins from separate domains because their comm… Show more
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