Ligation or External Fistulation of the Common Bile Duct in the Rat. II. Intestinal Disaccharidase Activities

Abstract

72 h after ligation or external fistulation of the common bile duct the activities of maltase, sucrase and lactase in the homogenate of the small intestinal mucosa of the rat were determined. The experiments were performed in connexion with intestinal perfusion studies, and the disaccharidase activities were measured in unperfused intestinal segments as well as in intestinal loops which had previously been perfused with a sucrose-containing solution. After bile duct ligation, the sucrase and maltase activities in a previously perfused intestinal loop were not different from those in sham-operated animals, the lactase activity was diminished. In a nonperfused segment, the sucrase activity was greater, the maltase activity was unchanged, and the lactase activity was lower than in control animals. After bile duct fistulation, the sucrase, maltase and lactase activities in a perfused segment were lower than in sham-operated rats. In a nonperfused loop, the sucrase activity was greater, the maltase activity was unchanged, and the lactase activity was lower then in the corresponding control group. These data suggest that bile is a factor which influences the total mucosal disaccharidase activities and, probably, the intracellular enzyme distribution.

Disacchandase Levels of the Rat Jejunum Are Altered by Dietary Fibre

“…The work of Bohmer [2] suggests that bile flow into the small bowel lumen has an effect on disaccharidase levels, while the influence of pancreatic secretions on disaccharidase levels cannot be excluded [18,19], We sug gest that the influence of dietary fibre compo nents on disaccharidase levels may reflect changes in bile or pancreatic secretions.…”

Effects of Dietary Fat and Gel-Forming Substances on Rat Jejunal Disaccharidase Levels

“…Bile flow [4] and pancreatic secretions [26,27] influence disaccharidase levels. Pan creatic proteases may degrade disacchari dases at the brush border [1], Additionally, pancreatic enzymes or bile may release brush border enzymes from the membrane itself [11,21].…”

“…McCarthy et al [16] have reported that high fat diets are associated with low disac charidase levels and other studies suggest that bile flow [4] and pancreatic secretions [26,27] influence disaccharidase levels in the small bowel mucosa. It is possible, therefore, that this influence of fat in the diet on disac charidases is due to the stimulation by di etary fat of bile flow [23] and pancreatic secretions [9,10].…”

Approaching the Sarcopenic Patient with Nonalcoholic Steatohepatitis-related Cirrhosis

“… 64 , 65 The obesity-related gut microbiome was shown to increase intestinal permeability and the secretion of lipopolysaccharide. 65 Similarly, the gut dysregulation observed in cirrhosis is characterized by increased intestinal permeability and endotoxemia 66 , 67 that contribute to chronic inflammation, oxidative stress, and insulin resistance during sarcopenia development and progression.…”