Ligature-Induced Periodontitis Increased Insulin Resistance and Triglyceride Levels in Wistar Rats

Tanaka, Hideki; Nakai, Kumiko; Murakami, Fumiko; Morita, Toyoko; Yamazaki, Yoji; Matsuike, Rieko; Shibata, Chika; Nagasaki, Masao; Kanda, Mai; Tanabe, Natsuko; Kawato, Takayuki; Maeno, Masao

doi:10.2485/jhtb.26.261

Cited by 4 publications

(4 citation statements)

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“…Furthermore, similarly to other infectious or chronic inflammatory diseases [59], periodontal diseases might alter concentration and composition of plasma lipids and lipoproteins [60], promoting atherosclerosis and NAFLD progression. In fact, studies on different experimental periodontitis rodent models suggest that this condition led to increases in TGs [61,62] and total cholesterol and to a decrease in HDL-cholesterol [62,63]. However, Ebersole et al [64] reported an increase in serum cholesterol, but no change in TGs in a non-human primate model of ligature-induced periodontitis.…”

Section: Discussionmentioning

confidence: 99%

A Diet Rich in Saturated Fat and Cholesterol Aggravates the Effect of Bacterial Lipopolysaccharide on Alveolar Bone Loss in a Rabbit Model of Periodontal Disease

et al. 2020

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Increasing evidence connects periodontitis with a variety of systemic diseases, including metabolic syndrome, atherosclerosis, and non-alcoholic fatty liver disease (NAFLD). The proposal of this study was to evaluate the role of diets rich in saturated fat and cholesterol in some aspects of periodontal diseases in a lipopolysaccharide (LPS)-induced model of periodontal disease in rabbits and to assess the influence of a periodontal intervention on hyperlipidemia, atherosclerosis, and NAFLD progression to non-alcoholic steatohepatitis. Male rabbits were maintained on a commercial standard diet or a diet rich in saturated fat (3% lard w/w) and cholesterol (1.3% w/w) (HFD) for 40 days. Half of the rabbits on each diet were treated 2 days per week with intragingival injections of LPS from Porphyromonas gingivalis. Morphometric analyses revealed that LPS induced higher alveolar bone loss (ABL) around the first premolar in animals receiving standard diets, which was exacerbated by the HFD diet. A higher score of acinar inflammation in the liver and higher blood levels of triglycerides and phospholipids were found in HFD-fed rabbits receiving LPS. These results suggest that certain dietary habits can exacerbate some aspects of periodontitis and that bad periodontal health can contribute to dyslipidemia and promote NAFLD progression, but only under certain conditions.

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Section: Discussionmentioning

confidence: 99%

A Diet Rich in Saturated Fat and Cholesterol Aggravates the Effect of Bacterial Lipopolysaccharide on Alveolar Bone Loss in a Rabbit Model of Periodontal Disease

et al. 2020

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“…In an in vitro study, stimulation of adipocytes with CRP and inflammatory cytokines led to enhanced differentiation of adipocytes and elevated production of maturation-promoting proteinases such as matrix metalloproteinases [34]. Furthermore, induced experimental periodontitis in rats resulted in elevated levels of the monocyte chemotactic factor (monocyte chemoattractant protein-1) in serum and lipids in the liver [35]. Therefore, considering these epidemiological, in vitro , and animal data, our results suggest that good toothbrushing practices are effective in preventing periodontitis and, consequently, in reducing the risk of developing obesity.…”

Section: Discussionmentioning

confidence: 99%

Effect of Periodontitis and Toothbrushing Frequency on Obesity Onset: A Cohort Study

Morita¹,

Yamazaki²,

Seto

et al. 2019

Med Sci Monit

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Background: The interplay between obesity and periodontitis has been widely examined. While obesity was reported as a risk factor for periodontitis, the inverse relationship is still little explored. Therefore, we aimed to determine whether periodontitis and toothbrushing frequency affect the onset of obesity. Material/Methods: This cohort study included 1619 employees of a business enterprise headquartered in Tokyo, who in 2002 and 2006 underwent in prescribed annual health checks, both general and dental-specific, and who were not obese in 2002 (body mass index <25). The response variable was obesity (or absence) at 4 years, while the explanatory variables were presence/absence of periodontal pockets and toothbrushing frequency in 2002; their relationships were examined by multiple logistic regression analysis. Results: Subjects with periodontal pockets ³4 mm showed a significantly higher odds ratio (OR) for onset of obesity at 4 years than those without periodontal pockets [OR: 1.59, 95% CI (confidence interval): 1.08-2.35, p<0.05]. Similarly, subjects who brushed their teeth ³3 times/day had a significantly lower obesity OR than those who brushed £1 time/day (OR: 0.49, 95% CI: 0.28-0.85, p<0.01). Conclusions: The presence of periodontal pockets and toothbrushing frequency are significantly associated with the onset of obesity. Periodontal pockets ³4 mm are associated with increased risk of obesity, while frequent toothbrushing (³3 times/day) appears to reduce the risk of obesity.

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“…The ligature-induced periodontitis model has been used frequently in relatively large animals, including rodents, to assess the host response and its effects on the tooth-supporting tissues (gingiva and bone) under well-controlled conditions. The use of a ligature-induced periodontitis model is reliable and reproducible over a period of 10 days in rodents [ 14 ]. The effect of metformin on a periodontal disease experimental model was previously confirmed by our group [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Metformin Hydrochloride-Loaded PLGA Nanoparticle in Periodontal Disease Experimental Model Using Diabetic Rats

Pereira

Brito²,

Lima

et al. 2018

IJMS

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Evidence shows that metformin is an antidiabetic drug, which can exert favorable anti-inflammatory effects and decreased bone loss. The development of nanoparticles for metformin might be useful for increased therapeutic efficacy. The aim of this study was to evaluate the effect of metformin hydrochloride-loaded Poly (d,l-Lactide-co-glycolide) (PLGA)/(MET-loaded PLGA) on a ligature-induced periodontitis model in diabetic rats. MET-loaded PLGA were characterized by mean diameter, particle size, polydispensity index, and entrapment efficiency. Maxillae were scanned using Microcomputed Tomography (µCT) and histopathological and immunohistochemical analysis. IL-1β and TNF-α levels were analyzed by ELISA immunoassay. Quantitative RT-PCR was used (AMPK, NF-κB p65, HMGB1, and TAK-1). The mean diameter of MET-loaded PLGA nanoparticles was in a range of 457.1 ± 48.9 nm (p < 0.05) with a polydispersity index of 0.285 (p < 0.05), Z potential of 8.16 ± 1.1 mV (p < 0.01), and entrapment efficiency (EE) of 66.7 ± 3.73. Treatment with MET-loaded PLGA 10 mg/kg showed low inflammatory cells, weak staining by RANKL, cathepsin K, OPG, and osteocalcin, and levels of IL-1β and TNF-α (p < 0.05), increased AMPK expression gene (p < 0.05) and decreased NF-κB p65, HMGB1, and TAK-1 (p < 0.05). It is concluded that MET-loaded PLGA decreased inflammation and bone loss in periodontitis in diabetic rats.

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Ligature-Induced Periodontitis Increased Insulin Resistance and Triglyceride Levels in Wistar Rats

Cited by 4 publications

References 35 publications

A Diet Rich in Saturated Fat and Cholesterol Aggravates the Effect of Bacterial Lipopolysaccharide on Alveolar Bone Loss in a Rabbit Model of Periodontal Disease

A Diet Rich in Saturated Fat and Cholesterol Aggravates the Effect of Bacterial Lipopolysaccharide on Alveolar Bone Loss in a Rabbit Model of Periodontal Disease

Effect of Periodontitis and Toothbrushing Frequency on Obesity Onset: A Cohort Study

Metformin Hydrochloride-Loaded PLGA Nanoparticle in Periodontal Disease Experimental Model Using Diabetic Rats

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