Light-Activated Gene Expression

Cruz, Federico G.; Koh, John T.; Link, Kristian H.

doi:10.1021/ja001804h

Cited by 84 publications

(66 citation statements)

References 19 publications

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“…Based on the growing understanding of the structural features required for effective RNA interference, we are currently exploring the positional and steric factors that may allow complete caging of siRNA. [6,[9][10][11] Previous efforts at light-mediated control of gene expression include the caging of hormones, [12] mRNA, [8] whole plasmids, [5] and ribozymes. [13] A potential advantage of our approach is that it utilizes the generality found in RNA interference.…”

Section: Angewandte Chemiementioning

confidence: 99%

Light‐Activated RNA Interference

2005

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Section: Angewandte Chemiementioning

confidence: 99%

Light‐Activated RNA Interference

2005

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“…14 In addition, a lot of caged biological active compounds have been prepared by using ONB phototrigger groups. [15][16][17][18][19][20] However, most of the known ONB-caged chromophore showed UV absorption, which is the major drawback and limitation for the practical application, as the presence of UV light provides poor penetration due to light scattering and absorbance by intrinsic polymer chromophores. Recently, Jullien and Bolze groups reported that the elongation of the conjugation backbone for the nitro group and the introduction of Dp-A structure would redshift the maximum wavelength of absorption and enlarge the corresponding molar absorption coefficient as well as the two-photon absorption (TPA) cross-sections.…”

Section: Introductionmentioning

confidence: 99%

Micropatterning of polymethacrylates by single‐ or two‐photon irradiation using π‐conjugated o‐nitrobenzyl ester phototrigger as side chains

Jin

Hong

et al. 2013

J of Applied Polymer Sci

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A new single-/two-photon sensitive monomer, (E)-5-(4-ethoxystyryl)22-nitrobenzyl methacrylate (ENbMA), was synthesized and copolymerized with methyl methacrylate (MMA) to form a series of photosensitive copolymers P(ENbMA-MMA)s that were well characterized by 1 H NMR and GPC. The photochemical and photophysical properties of both photosensitive monomer and copolymers upon visible light irradiation were studied by UV-Vis, FTIR, and HPLC spectra, which confirmed that 5-(4-ethoxystyryl)-2-nitrobenzyl ester can be photolyzed effectively with generation of the corresponding 5-(4-ethoxystyryl)-2-nitrosobenzaldehyde and carboxylic acid groups. The successful photocleavage endowed the optimized copolymers with excellent micropatterning property due to the effective generation of alkaline-soluble carboxylic acid groups. Moreover, the high two-photon absorption cross-sections (over 20 GM at 800 nm) and the comparable photolysis upon two-photon NIR light irradiation of the chromophores provided the copolymers with significant application in two-photon microfabrication.

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“…Recently, we initiated a program to explore the use of photocaged ligands of the nuclear and steroid hormone receptors as a general method to provide spatial and temporal control of gene expression. [14,15] Nuclear and steroid hormone receptors function as liganddependent transcriptional regulators to control the expression of a diverse array of genes involved in development and homeostasis. We have shown that photocaged analogues of estrogen receptor, retinoic acid receptor, and the thyroid hormone receptor agonists can mediate exposure-dependent transcription of hormone-responsive genes with different transcription responses.…”

Section: Introductionmentioning

confidence: 99%

“…We have shown that photocaged analogues of estrogen receptor, retinoic acid receptor, and the thyroid hormone receptor agonists can mediate exposure-dependent transcription of hormone-responsive genes with different transcription responses. [14,15] The activation of hormone receptors is unique in that the ™phototransducer∫ is a readily diffusible small molecule. Therefore, the system only becomes transiently light-sensitive after addition of the caged agonist.…”

Section: Introductionmentioning

confidence: 99%

Light‐Activated Transcription and Repression by Using Photocaged SERMs

Shi¹,

Koh²

2004

ChemBioChem

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Recently developed methods to regulate the spatial and temporal patterning of genes in a light-directed manner hold promise as powerful tools for exploring the function of genes that act through their unique spatiotemporal patterning. To further explore the application of photocaged ligands of nuclear receptors to control gene expression patterning, the actions of photocaged analogues of selective estrogen-receptor modulators (SERMs) have been evaluated. Photocaged derivatives of hydroxytamoxifen (NB-Htam) and guanidine tamoxifen (NB-Gtam) have been synthesized that selectively antagonize ER alpha- and ER beta-mediated transcription at classic estrogen response elements (EREs) in response to light. When present only intracellularly, Htam and Gtam provide a similar transient repression response. When SERMs are allowed to diffuse out of the cell, transcription is recovered at a similar rate for Htam and Gtam (6.4 and 5.6 h(-1)), but is notably faster than is observed with the covalently binding SERM tamoxifen aziridine (Taz) (3.8 h(-1)). This suggests that the duration of agonist action is controlled by ligand off-rates/diffusion and not by receptor turnover. Gtam activates ER beta-mediated transcription at AP1 sites in a similar way to what has previously been reported for Htam. NB-Gtam and NB-Tam provide a light-activated transcription response at AP1-driven reporters, thus illustrating the unique ability of photocaged SERMs to simultaneously mediate light-activated transcription and repression.

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Light-Activated Gene Expression

Cited by 84 publications

References 19 publications

Light‐Activated RNA Interference

Light‐Activated RNA Interference

Micropatterning of polymethacrylates by single‐ or two‐photon irradiation using π‐conjugated o‐nitrobenzyl ester phototrigger as side chains

Light‐Activated Transcription and Repression by Using Photocaged SERMs

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