Light-Addressable Electrochemical Sensors toward Spatially Resolved Biosensing and Imaging Applications

Meng, Yao; Chen, Fang‐Ming; Wu, Chunsheng; Krause, Steffi; Wang, Jian; Zhang, De-Wen

doi:10.1021/acssensors.2c00940

Cited by 23 publications

(18 citation statements)

References 156 publications

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“…We next sought to understand how SWV would impact LAE sensors when they are partially illuminated with varying amounts of power. This type of experimental condition is used when imaging ,, and performing array-type measurements , with LAE sensors. Under these conditions, the dark currents are proportional to the electrolyte contact area, while the illuminated currents will be proportional to the illuminated area.…”

Section: Resultsmentioning

confidence: 99%

Square-Wave Voltammetry Enables Measurement of Light-Activated Oxidations and Reductions on n-Type Semiconductor/Metal Junction Light-Addressable Electrochemical Sensors

et al. 2023

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Light-addressable electrochemical (LAE) sensing is a photoelectrochemical technique that enables high-density, individually addressed electrochemical measurements using light to activate an electrochemical reaction at the surface of a semiconducting photoelectrode. However, one major challenge is that only one electrochemical reaction (oxidation or reduction) will be activated by light. Here, we used square-wave voltammetry (SWV) to enable measurement of both types of electrochemical reactions using n-Si/Au NP LAE sensors. We demonstrated this approach for the oxidation of ferrocene methanol and the reduction of ruthenium hexamine and methylene blue. We found that for all molecules, SWV showed dramatic improvements in current under illumination in comparison with dark samples. We also demonstrated that this approach works for both fully illuminated and partially illuminated samples. Altogether, we hope these results open up new applications for LAE sensors, especially those based on semiconductor/metal junctions.

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Section: Resultsmentioning

confidence: 99%

Square-Wave Voltammetry Enables Measurement of Light-Activated Oxidations and Reductions on n-Type Semiconductor/Metal Junction Light-Addressable Electrochemical Sensors

et al. 2023

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“…This type of experimental condition is used when imaging 22,27,76,77 and performing array-type measurements 2,4 with LAE sensors. Under these conditions, the dark currents are proportional to the electrolyte contact area, while the illuminated currents will be proportional to the illuminated area.…”

Section: Resultsmentioning

confidence: 99%

“…2 There are a number of LAES modes including those based on the electrochemical techniques of potentiometry, alternating current impedance, and direct current amperometry/voltammetry. Each mode has different requirements of the semiconductor/solution interface, as discussed in the recent review by Meng et al 27 In this manuscript, we focus on LAES that involve faradaic electron transfer between a semiconductor and a freely diffusing redox species in solution.…”

Section: Introductionmentioning

confidence: 99%

Square wave voltammetry enables measurement of light-activated oxidations and reductions on n-type semiconductor/metal junction light-addressable electrochemical sensors

Arthur

Ali

Hussain

et al. 2023

Preprint

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Light-addressable electrochemical sensing (LAES) is a photoelectrochemical technique that enables high-density, individually addressed electrochemical measurements using light to activate an electrochemical reaction at the surface of a semiconducting photoelectrode. However, one major challenge is that only one electrochemical reaction (oxidation or reduction) will be activated by light. Here, we use square wave voltammetry (SWV) to enable measurements of both types of electrochemical reactions using n-Si/Au NP LAE sensors. We demonstrated this approach for the oxidation of ferrocene methanol and the reductions of ruthenium hexamine and methylene blue. We found that for all molecules, SWV showed dramatic improvements in current under illumination in comparison with dark samples. We also demonstrate that this approach works for both fully illuminated and partially illuminated samples. Altogether, we hope these results open up new applications for LAE sensors, especially those based on semiconductor/metal junctions.

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“…19 Another promising candidate for a label-free and single-cellbased approach is the light-addressable electrochemical sensor (LAES). 20 In a LAES, a flat, featureless semiconductor substrate is irradiated locally to generate local photocurrents, thereby achieving spatially resolved electrochemical recordings without any complicated connecting wires or microelectrode probes. Due to the unique light addressability, the LAES has been extensively developed for multiplexed detection and bio(chemical) imaging.…”

Section: ■ Introductionmentioning

confidence: 99%

Label-Free Imaging of Cell Apoptosis by a Light-Addressable Electrochemical Sensor

et al. 2023

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Label-free electrochemical visualization of cancer cell apoptosis is essential for cancer therapies. In this work, we proposed a noninvasive imaging method using a light-addressable electrochemical sensor (LAES) for label-free imaging of drug-induced tumor cell apoptosis. The dynamic AC photocurrent changes on MCF-7 human breast adenocarcinoma cells after inducing by tamoxifen were imaged. And the reasons for photocurrent changes on the cells were explored by monitoring the changes in the ζ potentials of cells and Faradic impedance. The results demonstrated that the AC photocurrent on apoptotic MCF-7 cells increased, and the apoptosis degree of each cell was heterogeneous. Moreover, the AC photocurrent increase was attributed to the increased cell membrane permeability and the increased gap between the cell basal surface and the substrate caused by cell apoptosis. This study provides a brand new approach for label-free visualizing cell apoptosis heterogeneity, which has great potential in apoptosis-associated drug screening or drug efficacy evaluation.

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Light-Addressable Electrochemical Sensors toward Spatially Resolved Biosensing and Imaging Applications

Cited by 23 publications

References 156 publications

Square-Wave Voltammetry Enables Measurement of Light-Activated Oxidations and Reductions on n-Type Semiconductor/Metal Junction Light-Addressable Electrochemical Sensors

Square-Wave Voltammetry Enables Measurement of Light-Activated Oxidations and Reductions on n-Type Semiconductor/Metal Junction Light-Addressable Electrochemical Sensors

Square wave voltammetry enables measurement of light-activated oxidations and reductions on n-type semiconductor/metal junction light-addressable electrochemical sensors

Label-Free Imaging of Cell Apoptosis by a Light-Addressable Electrochemical Sensor

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