Light and electron microscope observation of virus-induced Tetrahymena pyriformis in newborn mice (Mus musculus albinicus) brain

Teras, J; Entzeroth, Rolf; Scholtyseck, Erich; Kesa, L.; Schrauf, I.

doi:10.1007/bf00539569

Cited by 5 publications

(3 citation statements)

References 16 publications

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“…At the same time the issue of the long term persistence of picornaviruses in Paramecium with minor release of viruses in the medium remains open. The information about existence of picornaviruses as above is mentioned in scientific literature before [19]. One of the main conclusions of our research is that the presence of picornaviruses impacts on population of infusorians leading to sharp increase of their number in the medium.…”

mentioning

confidence: 68%

Interaction of Paramecium caudatum and Picornaviruses

et al. 2012

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In our paper we have researched the relationship between picornaviruses (poliovirus, foot-and-mouth disease virus and encephalomyocarditis virus) and Ciliata (Paramecium caudatum). We show that the number of Paramecium in medium sharply increased during coincubation with picornaviruses within 2-5 days. This cannot be explained only by the fact that viruses were nutrient source for Paramecium because in case of inactivated viruses the number of infusorians in medium increased a little. At the same time the titer of viruses harshly decreased whereas in the control group, which is free of Paramecium, the fall of titer was little. Picornaviruses were eliminated from medium if only living Parameciums were present in medium. After 7-9 days of coincubation only a few number of viruses were liberated from destroyed Parameciums. These results will be especially useful for management of reservoirs of picornaviruses in water and prevention of diseases.

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confidence: 68%

Interaction of Paramecium caudatum and Picornaviruses

et al. 2012

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“…According to the ICH Guidance for Industry S6 (Preclinical Safety Evaluation of Biotechnology-Derived Pharmaceuticals) concerns may arise from the presence of impurities or contaminants. Pathogenic effects of injecting living Tetrahymena pyriformis cells directly into mice brains might be attributable to a potential presence of viruses [190]. In general, Tetrahymena thermophila and Leishmania tarentolae are non-pathogenic protozoan species, which do not harm mammals or humans.…”

Section: Regulatory Aspects Of Protozoan Production Organismmentioning

confidence: 99%

Suspension Culture of Protozoan Organisms

Hartmann¹,

Breitling

2014

Industrial Scale Suspension Culture of Living Cells

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“…Subsequently, laboratory investigations were done, commonly with Tetrahymena. The viruses included influenza virus [74], encephalomyocarditis (EMC) virus [75], measles virus [75], vaccinia virus [76], coxsackie B-5 virus [77], adenovirus 3 [78], poliovirus [79], and Simian rotavirus SA11 [80]. Usually the ciliates were either assumed or demonstrated not to support production of these mammalian viruses [78,80] but sometimes the results were equivocal [75,77].…”

Section: Ciliates Interacting With Fish Microbial and Viral Pathogensmentioning

confidence: 99%

Use of Cell Cultures to Study the Interactions of Ciliates with Fish

Pinheiro¹,

Bols²

2013

Springer Science Reviews

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How the ciliates of fish can be cultured and be used to study ciliate/fish interactions are reviewed. The culturing of ciliates is currently done in basal solutions based on either freshwater, seawater, or bodily fluids of vertebrates. These are supplemented either with bacteria, fish cells, or organic matter, which can be defined or more commonly undefined, with proteose peptone being a prominent example. Among pathogenic ciliates, the most difficult to culture has been Ichthyophthirius multifiliis. To contrast, Cryptocaryon irritans caused a similar disease and has been maintained successfully in co-cultures with fish cells. Pathogenic scuticociliates and tetrahymenas are more amenable to culture, and can be grown axenically. These cultures have been used to study pathogenic mechanisms and to screen drugs for their potential chemotherapeutic value. Ciliates may act directly on fish to cause disease, but may also influence fish health indirectly through their interactions with other types of fish pathogens, for example bacteria and fungi. For example, in culture Tetrahymena spp. have been shown to phagocytose pathogenic bacteria, Yersinia ruckerii, and microsporidia, Glugea hertwigi, where ciliates are not linked to disease, very different ciliate/fish interactions are possible. For some fish larvae, free-living ciliates are a source of nutrients. Large-scale cultures of both freshwater and marine ciliates have been achieved and could be a source of feed for fish larvae in aquaculture. Finally, ciliates have the potential to feed on fish carcasses and in doing so make nutrients available to the ecosystem. In the future cell cultures should be invaluable in studying these and other possible relationships between fish and ciliates.

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Light and electron microscope observation of virus-induced Tetrahymena pyriformis in newborn mice (Mus musculus albinicus) brain

Cited by 5 publications

References 16 publications

Interaction of Paramecium caudatum and Picornaviruses

Interaction of Paramecium caudatum and Picornaviruses

Suspension Culture of Protozoan Organisms

Use of Cell Cultures to Study the Interactions of Ciliates with Fish

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