Light and electron microscopic studies on the Y organ of the freshwater crab Travancoriana schirnerae

Devi, A.R. Sudha; Smija, M. K.; Sagar, B K Chandrasekhar

doi:10.1016/j.jmau.2015.03.004

Cited by 7 publications

(2 citation statements)

References 36 publications

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“…The mitochondria due to its unique highest membrane structure packing per volume are ideal object for such study. This is due to the presence of cristae, the mitochondrial inner membrane structure, ranging in size from 100 to 500 nm 51–53 . Two cell types, a human cancer and non-cancerous animal, were chosen for our experiments, correspondently human colorectal cancer cell line HCT116 (ATCC CCL-247TM, Manassas, VA, USA) and the Chinese hamster ovary cell line CHO-K1 cells (ATCC CCL-61TM, Manassas, VA, USA).…”

Section: Methodsmentioning

confidence: 99%

The light-oxygen effect in biological cells enhanced by highly localized surface plasmon-polaritons

Khokhlova

Zolotovskii

Sokolovski

et al. 2019

Sci Rep

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Here at the first time we suggested that the surface plasmon-polariton phenomenon which it is well described in metallic nanostructures could also be used for explanation of the unexpectedly strong oxidative effects of the low-intensity laser irradiation in living matters (cells, tissues, organism). We demonstrated that the narrow-band laser emitting at 1265 nm could generate significant amount of the reactive oxygen species (ROS) in both HCT116 and CHO-K1 cell cultures. Such cellular ROS effects could be explained through the generation of highly localized plasmon-polaritons on the surface of mitochondrial crista. Our experimental conditions, the low-intensity irradiation, the narrow spectrum band (<4 nm) of the laser and comparably small size bio-structures (~10 μm) were shown to be sufficient for the plasmon-polariton generation and strong laser field confinement enabling the oxidative stress observed.

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Section: Methodsmentioning

confidence: 99%

The light-oxygen effect in biological cells enhanced by highly localized surface plasmon-polaritons

Khokhlova

Zolotovskii

Sokolovski

et al. 2019

Sci Rep

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“…Travancoriana schirnerae Bott, 1969 is an edible freshwater crab commonly distributed in the wetlands of Wayanad, Kerala, India. Previous studies on ultrastructure of the premoult YO of this species have shown numerous polymorphic mitochondria with tubular cristae, highly anastomosed tubules and vesicles of SER and abundant free ribosomes (Sudha Devi et al, 2015). Further studies on moult cyclerelated and destalked crab YO histology in the same species revealed cellular hypertrophy, cytoplasmic granulations and secretory vesicles (Smija and Sudha Devi, 2016).…”

Section: Effect Of 20e and Mf On Y-organ Histomorphologymentioning

confidence: 83%

Effect of 20-OH ecdysone and methyl farnesoate on histomorphology of the Y-organ during late intermoult and postmoult stages in the freshwater crab Travancoriana schirnerae Bott, 1969 (Crustacea: Gecarcinucidae)

Ayanath¹,

Raghavan²

2021

Nauplius

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This study investigated the effects of 20-OH ecdysone (20E) and methyl farnesoate (MF) administration on histomorphology of the Y-organ (YO) during late intermoult (C3 and C4) and early postmoult stages in the edible freshwater crab Travancoriana schirnerae Bott, 1969, widely distributed in the wetlands of Wayanad, Kerala, India. Histomorphological analyses of the 20E and MF administered crabs revealed that both 20E and MF were effective in inducing significant changes in YO during the late intermoult (P < 0.001) and early postmoult (P < 0.05) stages, as evidenced from a significant rise in the YO index (YO index = wet weight of YO/body weight of crab × 100), size of the gland and lobules, height of the lobular epithelium, cellular hypertrophy, presence of secretory vesicles and abundance of hemocytes. The results also indicated that the effects were more pronounced during the late intermoult stages than the postmoult stage and in the 20E administered crabs than the MF administered individuals. The percent increments in YO index, length and width of the gland and lobules and thickness of the lobular epithelium in 20E and MF injected crabs during the late intermoult stages were 25.95, 20.

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Identification and Characterization of the Y‐Organ of Orange Mud Crab Scylla Olivacea

Achdiat,

Fujaya,

Fazhan

et al. 2024

Microscopy Res & Technique

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The Y‐organs (YOs) synthesize and secrete molting hormones, and thus regulate crustacean growth and reproduction. However, the YOs of the orange mud crab Scylla olivacea is yet to be described due to its minute size and ambiguous feature. This study describes the location, morphological characteristics, histology, and the changes of YOs at different molt stages, and examines in vitro ecdysteroid secreted by the YOs of S. olivacea. Mature male crabs (> 90 mm carapace width, CW) were used to identify the location of the YOs while male juveniles (50–65 mm CW) at three molt stages (intermoult, premoult, postmoult) were dissected for the characterization of 20‐Hydroxyecdysone (20E) levels. The yellowish‐white, compact, and oval‐shaped YOs are located in pairs at the anterior cephalothorax region, beneath the mandibular external adductor muscle, anterior to the branchial chamber space, and around the end of the nerve tract. The YOs index and YO's cell diameter increased from postmoult to premoult; YO's diameter increased only during premoult stage. Under electron microscopy, the YO's surface morphology is rough, bumpy, network‐like, and porous, with several lacunar system tubules. High concentrations of haemolymph 20E during premoult correlated with the changes in the characteristics of YOs at this stage. The presence of ecdysteroid in YOs was confirmed by high performance liquid chromatography (HPLC). The characteristics of the YOs of S. olivacea are similar to the YO descriptions of other brachyurans. The successful identification and characterization of YOs will spearhead future research on understanding its role in molt regulation of mud crabs, consequently contributing to the development and progress of crab culture and soft‐shell crab industries.

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Light and electron microscopic studies on the Y organ of the freshwater crab Travancoriana schirnerae

Cited by 7 publications

References 36 publications

The light-oxygen effect in biological cells enhanced by highly localized surface plasmon-polaritons

The light-oxygen effect in biological cells enhanced by highly localized surface plasmon-polaritons

Effect of 20-OH ecdysone and methyl farnesoate on histomorphology of the Y-organ during late intermoult and postmoult stages in the freshwater crab Travancoriana schirnerae Bott, 1969 (Crustacea: Gecarcinucidae)

Identification and Characterization of the Y‐Organ of Orange Mud Crab Scylla Olivacea

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