Light and genotype effects on in vitro tuberization of potato plantlets

Dobránzki, J.; Tábori, K. M.; Ferenczy, A.

doi:10.1007/bf02358164

Cited by 11 publications

(5 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been known that various environmental and endogenous factors influence tuberization . A large number of studies have shown that short photoperiod, high light intensity, low temperature, low nitrogen levels and high sucrose levels can promote tuberization (Dobránzki et al 1999, Tadesse et al 2001, EL-Sawy et al 2007, Dobránszki and Tábori 2010. Phytohormones also play a crucial role in regulating the morphological events of tuberization (Aksenova et al 2012, Roumeliotis et al 2012.…”

Section: Introductionmentioning

confidence: 99%

Comparative proteomics illustrates the molecular mechanism of potato (Solanum tuberosum L.) tuberization inhibited by exogenous gibberellins in vitro

Cheng

Wang

Liu

et al. 2018

Physiologia Plantarum

View full text Add to dashboard Cite

Among the multiple environmental signals and hormonal factors regulating potato tuberization, gibberellins (GAs) are important components of the signaling pathways in these processes. To understand the GAs-signaling response mechanism of potato tuberization, a comparative proteomics approach was applied to analyze proteome change of potato tuberization in vitro subjected to a range of exogenous GA treatments (0, 0.01, 0.1 and 1.0 μM) using two-dimensional gel electrophoresis. Quantitative image analyses showed that a total of 37 protein spots have their abundance significantly altered more than 2-fold. Among these proteins, 13 proteins were up-regulated, 13 proteins were down-regulated, one protein was absent and 10 proteins were induced after treatment by exogenous GA . The MALDI-TOF/TOF MS analyses led to the identification of differentially abundant proteins that are mainly involved in bioenergy and metabolism, storage, signaling, cell defense and rescue, transcription, chaperones, transport. Furthermore, the comparative analysis of GA -responsive proteome allowed for general elucidation of underlying molecular mechanisms of potato tuberization inhibited by exogenous GA . Most of these cellular processes were not conducive to the transition from stolon elongation to tuber formation, including a blockage of starch and storage protein accumulation, the accelerated carbohydrate catabolism, a blockage of JA biosynthesis but an elevated endogenous GAs level, the amplification of GA signal transduction by other signaling pathways, and the regulation of cellular RNA metabolism for controlling tuberization. Our results firstly integrated physiology and proteome data to provide new insights into GA -signaling response mechanisms of potato tuberization in vitro.

show abstract

Section: Introductionmentioning

confidence: 99%

Comparative proteomics illustrates the molecular mechanism of potato (Solanum tuberosum L.) tuberization inhibited by exogenous gibberellins in vitro

Cheng

Wang

Liu

et al. 2018

Physiologia Plantarum

View full text Add to dashboard Cite

show abstract

“…Thus, an appropriate combination of light and dark conditions with short days can synchronize and accelerate the initiation and development of microtubers, as well as increase their numbers (Dobránszki, Tábóri, & Ferenczy, 1999).…”

Section: Resultsmentioning

confidence: 99%

Efecto del ácido bórico y la luz en el número y biomasa de microtubérculos de papa cv. “Floresta”

García-García¹,

Azofeifa-Bolaños

2017

View full text Add to dashboard Cite

In order to understand the performance of Solanum tuberosum L. "Floresta" in producing microtubers, the effect of four concentrations of boric acid (6.2 mg l ) under two conditions-1) 8 hour dark and 2) 16 hour light photoperiod and 8 hour dark-was evaluated under in vitro culture conditions. Full-strength Murashige and Skoog medium was used as a control. The data analysis for the number and biomass of microtubers were significant for the condition (p ≤ 0.05). Concerning the concentration of boric acid and the interaction between factors, a significant difference was obtained for biomass (p ≤ 0.05). In dark conditions, a greater number of microtubers was obtained, although the biomass was lower compared to the light conditions where the biomass was higher, and the number of microtubers was lower. A concentration of 9.3 mg l -1 of boric acid in both types of conditions was the best treatment to produce a greater number and more biomass of microtubers. The differences identified in this work with regards to the number and biomass of microtubers were probably the combined result of genotype and specific culture conditions. Even considering this, the use of lighting conditions is proposed to induce larger and greener microtubers.

show abstract

“…The cultivars were differed in their responses to photoperiod. Each genotype has a specific photoperiod requirement (Seabrook et al, 1993 andDobranszki et al, 1999) Regrinding the means of interaction between photoperiod and concentrations, there were no significant differences between means of concentrations either in dark or light condition.…”

Section: Effect Of Silver Thiosulphate (Sts) Concentrations On Numbermentioning

confidence: 98%

“…During incubation under light, GA3 is synthesized which inhibits tuber induction while darkness enhanced tuberonic acid synthesis, which plays important role in tuber formation. Dobranszki et al (1999).…”

Section: Effect Of Silver Thiosulphate (Sts) Concentrations On Numbermentioning

confidence: 99%

“…It was previously reported that the genotypes were differed in their responses to photoperiod. And each genotype has a specific photoperiod requirement (Seabrook et al, 1993 andDobranszki et al, 1999). Also Gopal et al (1998), who stated that it is important to develop genotype specific protocols for optimum microtuberization.…”

Section: Effect Of Silver Thiosulphate (Sts) Concentrations On Numbermentioning

confidence: 99%

See 1 more Smart Citation

Effect of Silver Thiosulphate and Photoperiod on in Vitro Tuberization of Three Potato (Solanum Tuberosum L.) Cultivars

El-Sayed¹,

Taha²,

Darwish³

et al. 2021

PLANT ARCHIVES

View full text Add to dashboard Cite

The experiment was carried out to study the effect of 6 concentrations of silver thiosulphate (STS) and photoperiod on in vitro tuberization of three potato cultivars, namely, Herms, Spunta and Santana. The experiment was conducted in tissue culture laboratory, Vegetable Crops Dep., Fac. Agric., Cairo Univ. Tip meristems in each cultivar were excised and cultured on MS media. In vitro plantlets of all cultivars were multiplied as per routine by sub culturing twice. In microtuberization stage, nodal cuttings from second subculture were cultured on MS media with 0, 0.4, 0.8, 1.2, 1.6 or 2 mM of STS. The treatments were kept under dark or light conditions. STS at 0.4 and 0.8 mM recorded the tallest microplants. The highest number of leaves and branches per plantlet were obtained with 0.4mM STS. On the other hand, the STS at 2 mM recorded the lowest values of plantlet height, number of leaves and branches per plantlet. The highest percentage of microtuberization was recorded with STS at 0.4 mM under dark conditions, and at 2 mM under light one. At dark, Spunta with 1.6 mM STS produced significantly the highest number of microtubers/plantlet. Whereas at light, the maximum number of microtubers/plantlet was obtained with 0.4, 0.8 and 1.2 mM STS in Herms, Spunta and Santans, respectively. In'Spunta', application of STS at 0.4 mM under light folded the number of microtubers/plantlet 2.9 compared with dark. In Santana, number of microtubers/plantlet of 0, 0.8, 1.2, 1.6 and 2 mM STS were higher in light comparing to dark. STS applied at 0.8 and 1.2 mM produced significantly the highest value of microtuber fresh weight in Spunta and Santana, respectively under dark. In 'Spunta', the applications with 0, 1.2, 1.6 and 2 mM STS at light conditions recorded fresh weight of microtuber higher than in dark.

show abstract

Light and genotype effects on in vitro tuberization of potato plantlets

Cited by 11 publications

References 10 publications

Comparative proteomics illustrates the molecular mechanism of potato (Solanum tuberosum L.) tuberization inhibited by exogenous gibberellins in vitro

Comparative proteomics illustrates the molecular mechanism of potato (Solanum tuberosum L.) tuberization inhibited by exogenous gibberellins in vitro

Efecto del ácido bórico y la luz en el número y biomasa de microtubérculos de papa cv. “Floresta”

Effect of Silver Thiosulphate and Photoperiod on in Vitro Tuberization of Three Potato (Solanum Tuberosum L.) Cultivars

Contact Info

Product

Resources

About