Light controlled cell-to-cell adhesion and chemical communication in minimal synthetic cells

Chakraborty, Taniya; Bartelt, Solveig Mareike; Steinkühler, Jan; Dimova, Rumiana; Wegner, Seraphine V.

doi:10.1039/c9cc04768a

Cited by 37 publications

(56 citation statements)

References 22 publications

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“…未来如果可以利用外界刺激使人工细胞内的ATP可以循环合成将是人工细胞能够实现可持续代谢的重要一步 [17] . 再如, 目前的刺激响应性的人工细胞间的通信行为的研究主要是化学物质作为通信信号, 较为单一, 拓宽通信信号的种类如产生光图 9 利用光来控制发送方和接收方人工细胞之间的粘附的示意图 [71] (网络版彩图)…”

Section: 人工细胞-细胞间粘附unclassified

Research progress in stimulus-responsive behaviors of artificial cells

Wang¹,

Xiao²,

Liu³

2022

Sci. Sin.-Chim

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Artificial cells with cell-like characteristics can be used as biomimetic systems to simulate the function and structure of real cells for further investigating the operation mechanism of cells in life. Cells can respond corresponding behaviors by sensing the signal stimulation from the external minimal interference, and then carry out various reactions related to biological activities. Thus, to study the stimulus-responsive behaviors of artificial cells not only provides an important theoretical significance to explore and understand properties of biological cells, but also demonstrates important potential applications in biosensors, disease diagnosis, drug delivery and so on. In this review, the recent research progress of stimuli-responsive behaviors of artificial cells, including the construction of stimuli-responsive artificial cells, the research on stimuli-responsive behaviors in regulating enzymatic reactions, material transport, communication and adhesion, is briefly introduced. Finally, current challenges and future perspectives for controlling artificial cells are discussed.

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Section: 人工细胞-细胞间粘附unclassified

Research progress in stimulus-responsive behaviors of artificial cells

Wang¹,

Xiao²,

Liu³

2022

Sci. Sin.-Chim

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“…Another innovative example of concentrated signal release upon synthetic cells' contact is the work of Chakraborty et al, where the synthetic cell adhesion between prey and predator populations is triggered upon bioluminescence from predator cells that, in turn, dimerizes proteins iLID and Nano, each of which resides in the membrane of one group of synthetic cells [141]. The dimerization reconstitutes synthetic cell adhesion, which leads to opening of αHL that is blocked unless synthetic cells are in contact [142]. The opening of αHL activates phospholipase A 2 (PLA2) inside the prey cells through diffusion of calcium ions from predator cells.…”

Section: Synthetic Cell-synthetic Cell Communicationmentioning

confidence: 99%

Synthetic Cell as a Platform for Understanding Membrane-Membrane Interactions

et al. 2021

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In the pursuit of understanding life, model membranes made of phospholipids were envisaged decades ago as a platform for the bottom-up study of biological processes. Micron-sized lipid vesicles have gained great acceptance as their bilayer membrane resembles the natural cell membrane. Important biological events involving membranes, such as membrane protein insertion, membrane fusion, and intercellular communication, will be highlighted in this review with recent research updates. We will first review different lipid bilayer platforms used for incorporation of integral membrane proteins and challenges associated with their functional reconstitution. We next discuss different methods for reconstitution of membrane fusion and compare their fusion efficiency. Lastly, we will highlight the importance and challenges of intercellular communication between synthetic cells and synthetic cells-to-natural cells. We will summarize the review by highlighting the challenges and opportunities associated with studying membrane–membrane interactions and possible future research directions.

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“…The giant unilamellar vesicles (GUVs) [ 5,63–67 ] are particularly attractive for synthetic cell due to their similar size to real cell. Various strategies have been developed to prepare GUVs.…”

Section: Formation Of Various Phospholipid Assembliesmentioning

confidence: 99%

Versatile Phospholipid Assemblies for Functional Synthetic Cells and Artificial Tissues

Wang

et al. 2020

Advanced Materials

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The bottom‐up construction of a synthetic cell from nonliving building blocks capable of mimicking cellular properties and behaviors helps to understand the particular biophysical properties and working mechanisms of a cell. A synthetic cell built in this way possesses defined chemical composition and structure. Since phospholipids are native biomembrane components, their assemblies are widely used to mimic cellular structures. Here, recent developments in the formation of versatile phospholipid assemblies are described, together with the applications of these assemblies for functional membranes (protein reconstituted giant unilamellar vesicles), spherical and nonspherical protoorganelles, and functional synthetic cells, as well as the high‐order hierarchical structures of artificial tissues. Their biomedical applications are also briefly summarized. Finally, the challenges and future directions in the field of synthetic cells and artificial tissues based on phospholipid assemblies are proposed.

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Light controlled cell-to-cell adhesion and chemical communication in minimal synthetic cells

Abstract: Light controlled adhesions between sender and receiver GUVs, used as minimal synthetic cells, photoregulates their spatial proximity and chemical communication.

Cited by 37 publications

References 22 publications

Research progress in stimulus-responsive behaviors of artificial cells

Research progress in stimulus-responsive behaviors of artificial cells

Synthetic Cell as a Platform for Understanding Membrane-Membrane Interactions

Versatile Phospholipid Assemblies for Functional Synthetic Cells and Artificial Tissues

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