Light-dependent cold-induced fatty acid unsaturation, changes in membrane fluidity, and alterations in gene expression in Synechocystis

Mironov, Kirill S.; Sidorov, Roman A.; Trofimova, M. S.; Bedbenov, Vladimir S.; Цыдендамбаев, В. Д.; Allakhverdiev, Suleyman I.

doi:10.1016/j.bbabio.2011.12.011

Cited by 77 publications

(38 citation statements)

References 29 publications

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“…The level of long chain fatty acids (C20) increased in proportion in 10 °C to 9.0% compared to 5.1% and 5.9% of 20 °C and 30 °C, respectively. These results correlate with previous studies that showed an increase in unsaturated fatty acid in response to low temperature to avoid a decrease in membrane fluidity182122. The increase of long chain fatty acids and PUFA levels in low-temperature conditions suggests changes in the structure of membrane-forming lipids, which changed to spatially larger forms to increase membrane fluidity21.…”

Section: Resultssupporting

confidence: 91%

Genome-wide transcriptome analysis revealed organelle specific responses to temperature variations in algae

Shin

Hong

Yoo

et al. 2016

Sci Rep

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Temperature is a critical environmental factor that affects microalgal growth. However, microalgal coping mechanisms for temperature variations are unclear. Here, we determined changes in transcriptome, total carbohydrate, total fatty acid methyl ester, and fatty acid composition of Tetraselmis sp. KCTC12432BP, a strain with a broad temperature tolerance range, to elucidate the tolerance mechanisms in response to large temperature variations. Owing to unavailability of genome sequence information, de novo transcriptome assembly coupled with BLAST analysis was performed using strand specific RNA-seq data. This resulted in 26,245 protein-coding transcripts, of which 83.7% could be annotated to putative functions. We identified more than 681 genes differentially expressed, suggesting an organelle-specific response to temperature variation. Among these, the genes related to the photosynthetic electron transfer chain, which are localized in the plastid thylakoid membrane, were upregulated at low temperature. However, the transcripts related to the electron transport chain and biosynthesis of phosphatidylethanolamine localized in mitochondria were upregulated at high temperature. These results show that the low energy uptake by repressed photosynthesis under low and high temperature conditions is compensated by different mechanisms, including photosystem I and mitochondrial oxidative phosphorylation, respectively. This study illustrates that microalgae tolerate different temperature conditions through organelle specific mechanisms.

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Section: Resultssupporting

confidence: 91%

Genome-wide transcriptome analysis revealed organelle specific responses to temperature variations in algae

Shin

Hong

Yoo

et al. 2016

Sci Rep

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“…Translating our findings for the different membranes into a biological context suggests that modulation of dimerization interfaces of transmembrane proteins can be achieved by changes of the lipid composition, which is a common stresses reaction [52,53,54]. Such dependence of protein action and membrane dynamics has for example been described for Hik33 [55,56,57] or DesK [58]. Furthermore, there are several reports that the transport or enzyme activity of proteins depends on the bilayer thickness or curvature (e.g., reviewed in [59]).…”

Section: Discussionmentioning

confidence: 59%

The Influence of Fatty Acids on the GpA Dimer Interface by Coarse-Grained Molecular Dynamics Simulation

Flinner¹,

Mirus²,

Schleiff³

2014

IJMS

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The hydrophobic thickness of membranes, which is manly defined by fatty acids, influences the packing of transmembrane domains of proteins and thus can modulate the activity of these proteins. We analyzed the dynamics of the dimerization of Glycophorin A (GpA) by molecular dynamics simulations to describe the fatty acid dependence of the transmembrane region assembly. GpA represents a well-established model for dimerization of single transmembrane helices containing a GxxxG motif in vitro and in silico. We performed simulations of the dynamics of the NMR-derived dimer as well as self-assembly simulations of monomers in membranes composed of different fatty acid chains and monitored the formed interfaces and their transitions. The observed dimeric interfaces, which also include the one known from NMR, are highly dynamic and converted into each other. The frequency of interface formation and the preferred transitions between interfaces similar to the interface observed by NMR analysis strongly depend on the fatty acid used to build the membrane. Molecular dynamic simulations after adaptation of the helix topology parameters to better represent NMR derived structures of single transmembrane helices yielded an enhanced occurrence of the interface determined by NMR in molecular dynamics simulations. Taken together we give insights into the influence of fatty acids and helix conformation on the dynamics of the transmembrane domain of GpA.

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“…Cold stress leads to an increase of unsaturated fatty acids as membrane phospholipids [20]. It was demonstrated that cold induced increases in desaturation of fatty acids leads to the membrane fluidity that is essential for cold acclimation of cells [31]. It was suggested that cold stress induced changes in membrane lipids might play a significant role in the response and adaptation of cells to environmental change.…”

Section: Response To Proteome Changes At Low Temperaturementioning

confidence: 99%

Proteomic analysis of Synechocystis sp. PCC6803 responses to low-temperature and high light conditions

Hong

Kim

Jang

et al. 2014

Biotechnol Bioproc E

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The global changes in protein expression of Synechocystis sp. PCC6803, a photosynthetic bacterium for the production of secondary metabolites as a green cell factory, were investigated by proteome separation and a subsequent tandem mass spectrometry. Two different proteome separation techniques, strong cation exchange chromatography and off-gel electrophoresis, were applied. The combination of the two proteome separation techniques enabled the comparative analysis of the differential regulation of the Synechocystis proteome in response to two different environmental factors, temperature and light. A total of 1,483 proteins were identified, which represent over 40% of the genes in Synechocystis. Our data showed that fatty acid metabolism was inhibited by (3R)-hydroxymyristol acyl carrier protein dehydrase (Sll1605) under low temperature conditions. The expression of UDP-N-acetylglucosamine acyltransferase (Sll0379) and 3-O- [3-hydroxymyristoyl] glucosamine N-acyltransferase (Slr0776), which is involved in lipopolysaccharide metabolism, was not observed under high light conditions. Under high light exposure, proteins related to iron-sulfur metabolism were detected, which may be responsible for maintaining the redox potential of the photosystem. High light under low temperature caused severe damage to the photosystem. Some of the responses to these stresses were similar to those previously reported for other photosynthetic organisms. Notably, this study revealed the followings: (i) low temperature inhibits fatty acid synthesis; (ii) high light inhibits lipopolysaccharides synthesis and stimulates the expression of iron-sulfur related proteins; and (iii) high light under low temperature induces the photorespiratory cycle. The global proteomic analysis clearly showed that stress conditions such as low temperature and/or high light induce cellular metabolisms related with the protection of their photosystems in the model microalga Synechocystis sp. PCC6803.

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Light-dependent cold-induced fatty acid unsaturation, changes in membrane fluidity, and alterations in gene expression in Synechocystis

Cited by 77 publications

References 29 publications

Genome-wide transcriptome analysis revealed organelle specific responses to temperature variations in algae

Genome-wide transcriptome analysis revealed organelle specific responses to temperature variations in algae

The Influence of Fatty Acids on the GpA Dimer Interface by Coarse-Grained Molecular Dynamics Simulation

Proteomic analysis of Synechocystis sp. PCC6803 responses to low-temperature and high light conditions

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