Light-Dependent Iron Transport into Isolated Barley Chloroplasts

Bughio, N.; Takahashi, Michiko; Yoshimura, Esturo; Nishizawa, Naoyuki; Mori, Satoshi

doi:10.1093/oxfordjournals.pcp.a029079

Cited by 54 publications

(34 citation statements)

References 12 publications

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“…This might explain the improvement of peanut Fe nutrition in maize /peanut intercropping system. Some have suggested shading can also have a great effect in improvement of Fe nutrition in plants (Pushnik and Miller 1982;Terry 1983;Bughio et al 1997), but during our experiment, rhizoboxes were often moved to allow adequate sunlight. Thus, shading from maize was negligible and not a contributing factor.…”

Section: Discussionmentioning

confidence: 99%

A study on the improvement iron nutrition of peanut intercropping with maize on nitrogen fixation at early stages of growth of peanut on a calcareous soil

Zuo

Liu

Zhang

et al. 2004

Soil Science and Plant Nutrition

105

130

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Section: Discussionmentioning

confidence: 99%

A study on the improvement iron nutrition of peanut intercropping with maize on nitrogen fixation at early stages of growth of peanut on a calcareous soil

Zuo

Liu

Zhang

et al. 2004

Soil Science and Plant Nutrition

105

130

View full text Add to dashboard Cite

“…Moreover, barley chloroplasts were shown, using radiolabeled iron, to influx iron during the day and to efflux it at night (Bughio et al, 1997). It was therefore suggested that a single transporter of the chloroplast envelope could work in both directions in response to light/dark conditions.…”

Section: Ysl4 and Ysl6 Specifically Transport Ironmentioning

confidence: 99%

The Arabidopsis YELLOW STRIPE LIKE4 and 6 Transporters Control Iron Release from the Chloroplast

et al. 2013

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In most plant cell types, the chloroplast represents the largest sink for iron, which is both essential for chloroplast metabolism and prone to cause oxidative damage. Here, we show that to buffer the potentially harmful effects of iron, besides ferritins for storage, the chloroplast is equipped with specific iron transporters that respond to iron toxicity by removing iron from the chloroplast. We describe two transporters of the YELLOW STRIPE1-LIKE family from Arabidopsis thaliana, YSL4 and YSL6, which are likely to fulfill this function. Knocking out both YSL4 and YSL6 greatly reduces the plant's ability to cope with excess iron. Biochemical and immunolocalization analyses showed that YSL6 resides in the chloroplast envelope. Elemental analysis and histochemical staining indicate that iron is trapped in the chloroplasts of the ysl4 ysl6 double mutants, which also accumulate ferritins. Also, vacuolar iron remobilization and NRAMP3/4 expression are inhibited. Furthermore, ubiquitous expression of YSL4 or YSL6 dramatically reduces plant tolerance to iron deficiency and decreases chloroplastic iron content. These data demonstrate a fundamental role for YSL4 and YSL6 in managing chloroplastic iron. YSL4 and YSL6 expression patterns support their physiological role in detoxifying iron during plastid dedifferentiation occurring in embryogenesis and senescence.

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“…In shoot tissues, 80-90 % of iron is found in chloroplasts (Terry and Abadía 1986;Morrissey and Guerinot 2009;Wilson and Connolly 2013), and thylakoid membranes contain~60 % of the iron found in leaf (Castagna et al 2009). Chloroplasts are able to transport Fe 3+ or Fe 2+ from the cytosol to the stroma (Bughio et al 1997;Shingles et al 2001). The first protein to be identified as playing a role in chloroplast iron acquisition was permease in chloroplast 1 (encoded by AtPIC1), which is also called translocon of inner chloroplast envelope 21 (TIC21; Teng et al 2006;Duy et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Tobacco PIC1 Mediates Iron Transport and Regulates Chloroplast Development

et al. 2014

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Iron is an essential element for plant growth and development, playing important roles in a variety of cellular activities including cell respiration, chlorophyll biosynthesis, and DNA synthesis. In chloroplasts, iron is essential for photosynthetic electron transport and functions as a cofactor for superoxide dismutases. Thus, iron homeostasis is critical for chloroplast and plant development. To better understand the mechanisms by which iron is transported into chloroplasts, we cloned and characterized the Nicotiana tabacum homolog of AtPIC1, which is a chloroplast iron transporter in Arabidopsis thaliana. NtPIC1 was expressed in many tissues of the tobacco plant, and the encoded protein was localized to the chloroplast envelope. Southern blotting indicated that there is a single copy of NtPIC1 in the tobacco genome. Moreover, yeast complementation assays suggested that NtPIC1 transports iron. We used RNA interference to downregulate NtPIC1 in transgenic plants, which resulted in albinism, dwarfism, iron-deficient chloroplasts, and chloroplast that exhibited ultrastructural defects. NtPIC1 overexpression resulted in deep-green leaves, elevated levels of chlorophyll, more densely packed chloroplasts, and the accumulation of iron and starch grains within chloroplasts. In addition, NtPIC1 influenced the expression of genes involved in iron transport, iron storage, iron oxidative stress, and the biogenesis of ironsulfur proteins. These results suggest that NtPIC1 transports iron into chloroplasts, regulates iron homeostasis, and influences plant development.

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Light-Dependent Iron Transport into Isolated Barley Chloroplasts

Cited by 54 publications

References 12 publications

A study on the improvement iron nutrition of peanut intercropping with maize on nitrogen fixation at early stages of growth of peanut on a calcareous soil

A study on the improvement iron nutrition of peanut intercropping with maize on nitrogen fixation at early stages of growth of peanut on a calcareous soil

The Arabidopsis YELLOW STRIPE LIKE4 and 6 Transporters Control Iron Release from the Chloroplast

Tobacco PIC1 Mediates Iron Transport and Regulates Chloroplast Development

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