Light-dependent regulation of the gene cut-1 of Neurospora, involved in the osmotic stress response

Youssar, Loubna; Ávalos, Javier

doi:10.1016/j.fgb.2006.05.003

Cited by 4 publications

(5 citation statements)

References 53 publications

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“…16C ). For example, cut-1 involved in osmo-sensing has been reported to be under WCC control [75] . Jones et al [57] have reported a role of rrg-1 in osmo-sensing reminiscent of the os mutants.…”

Section: Resultsmentioning

confidence: 99%

Systems Biology of the Clock in Neurospora crassa

et al. 2008

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A model-driven discovery process, Computing Life, is used to identify an ensemble of genetic networks that describe the biological clock. A clock mechanism involving the genes white-collar-1 and white-collar-2 (wc-1 and wc-2) that encode a transcriptional activator (as well as a blue-light receptor) and an oscillator frequency (frq) that encodes a cyclin that deactivates the activator is used to guide this discovery process through three cycles of microarray experiments. Central to this discovery process is a new methodology for the rational design of a Maximally Informative Next Experiment (MINE), based on the genetic network ensemble. In each experimentation cycle, the MINE approach is used to select the most informative new experiment in order to mine for clock-controlled genes, the outputs of the clock. As much as 25% of the N. crassa transcriptome appears to be under clock-control. Clock outputs include genes with products in DNA metabolism, ribosome biogenesis in RNA metabolism, cell cycle, protein metabolism, transport, carbon metabolism, isoprenoid (including carotenoid) biosynthesis, development, and varied signaling processes. Genes under the transcription factor complex WCC ( = WC-1/WC-2) control were resolved into four classes, circadian only (612 genes), light-responsive only (396), both circadian and light-responsive (328), and neither circadian nor light-responsive (987). In each of three cycles of microarray experiments data support that wc-1 and wc-2 are auto-regulated by WCC. Among 11,000 N. crassa genes a total of 295 genes, including a large fraction of phosphatases/kinases, appear to be under the immediate control of the FRQ oscillator as validated by 4 independent microarray experiments. Ribosomal RNA processing and assembly rather than its transcription appears to be under clock control, suggesting a new mechanism for the post-transcriptional control of clock-controlled genes.

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Section: Resultsmentioning

confidence: 99%

Systems Biology of the Clock in Neurospora crassa

et al. 2008

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“…It is allelic to ovc (overaccumulator of carotenoids) and encodes a putative phosphatase (Youssar et al, 2005). The expression of the cut-1 gene increases after hyperosmotic and heatshock treatment (Youssar & Avalos, 2006). Other osmosensitive mutants are known [e.g.…”

Section: Introductionmentioning

confidence: 99%

Turgor regulation in the osmosensitive cut mutant of Neurospora crassa

Lew

Levina

2007

Microbiology

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The internal hydrostatic pressure (turgor) of fungal cells is maintained at 400-500 kPa. The turgor is regulated by changes in ion flux and by production of the osmotically active metabolite glycerol. In Neurospora crassa, there are at least two genetically distinct pathways that function in adaptation to hyperosmotic shock. One involves a mitogen-activated protein (MAP) kinase cascade (kinases OS-4, OS-5 and OS-2 downstream of the osmosensing OS-1); the other is less understood, but involves the cut gene, which encodes a putative phosphatase. This study examined turgor regulation, electrical responses, ion fluxes and glycerol accumulation in the cut mutant. Turgor recovery after hyperosmotic treatment was similar to that in the wild-type, for both time-course (~40 min) and magnitude. Prior to turgor recovery, the hyperosmotic shock caused a rapid transient depolarization of the membrane potential, followed by a sustained hyperpolarization that occurred concomitant with increased H + efflux, indicating that the plasma membrane H + -ATPase was being activated. These changes also occurred in the wild-type. Net fluxes of Ca 2+and Cl " during turgor recovery were similar to those in the wild-type, but K + influx was attenuated in the cut mutant. The similar turgor recovery can be explained by the ion uptake, since glycerol did not accumulate in the cut mutant within the time frame of turgor recovery (but did accumulate in the wild-type). The results suggest that turgor regulation involves multi-faceted coordination of both ion flux and glycerol accumulation. Ion uptake is activated by a MAP kinase cascade, while CUT is required for glycerol accumulation.

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“…Expression of the gene cut-1 in N. crassa is downregulated by light and upregulated in the dark in mutants of the white collar (WC) complex (Youssar & Avalos, 2006). To investigate a possible regulatory effect of light on cutA and gldB mRNAs, dark-grown wild-type cultures were transferred to light and the effect of illumination time was investigated.…”

Section: Resultsmentioning

confidence: 99%

“…This is further supported by the rapid reduction of cutA mRNA content after hypo-osmotic shock, and its transient induction by illumination. However, photoinduction of cutA mRNA differs strikingly from the downregulation of cut-1 mRNA levels by light in N. crassa (Youssar & Avalos, 2006). Moreover, cut-1 mRNA content increases notably in the dark in different regulatory mutants involved in the light response, including those of the WC complex, an effect independent of the downregulation by light.…”

Section: Discussionmentioning

confidence: 96%

“…However, the single cut mutant exhibits normal carotenogenesis, indicating that the Ovc phenotype is due to the simultaneous loss of cut-1 and at least a second unidentified gene (Youssar & Avalos, 2007). Intriguingly, cut-1 gene expression is repressed by light and is controlled by proteins involved in light regulation in N. crassa (Youssar & Avalos, 2006), suggesting a possible link with the light-dependent phenotype of the ovc mutant.…”

Section: Introductionmentioning

confidence: 99%

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The gene cutA of Fusarium fujikuroi, encoding a protein of the haloacid dehalogenase family, is involved in osmotic stress and glycerol metabolism

2014

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Survival of micro-organisms in natural habitats depends on their ability to adapt to variations in osmotic conditions. We previously described the gene cut-1 of Neurospora crassa, encoding a protein of the haloacid dehalogenase family with an unknown function in the osmotic stress response. Here we report on the functional analysis of cutA, the orthologous gene in the phytopathogenic fungus Fusarium fujikuroi. cutA mRNA levels increased transiently after exposure to 0.68 M NaCl and were reduced upon return to normal osmotic conditions; deletion of the gene resulted in a partial reduction in tolerance to osmotic stress. ΔcutA mutants contained much lower intracellular levels of glycerol than the wild-type, and did not exhibit the increase following hyper-osmotic shock expected from the high osmolarity glycerol (HOG) response. cutA is linked and divergently transcribed with the putative glycerol dehydrogenase gene gldB, which showed the same regulation by osmotic shock. The intergenic cutA/gldB regulatory region contains putative stress-response elements conserved in other fungi, and both genes shared other regulatory features, such as induction by heat shock and by illumination. Photoinduction was also observed in the HOG response gene hogA, and was lost in mutants of the white collar gene wcoA. Previous data on glycerol production in Aspergillus spp. and features of the predicted CutA protein lead us to propose that F. fujikuroi produces glycerol from dihydroxyacetone, and that CutA is the enzyme involved in the synthesis of this precursor by dephosphorylation of dihydroxyacetone-3P.

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Light-dependent regulation of the gene cut-1 of Neurospora, involved in the osmotic stress response

Cited by 4 publications

References 53 publications

Systems Biology of the Clock in Neurospora crassa

Systems Biology of the Clock in Neurospora crassa

Turgor regulation in the osmosensitive cut mutant of Neurospora crassa

The gene cutA of Fusarium fujikuroi, encoding a protein of the haloacid dehalogenase family, is involved in osmotic stress and glycerol metabolism

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