Randomly dividing 160 cases into two groups, the research group was given conventional chemotherapy while the other was exposed to in vitro cultured calculus bovis, in order to evaluate the impact of this on the quality of life and immune function of those with non-small cell lung cancer undergoing chemotherapy. The clinical efficacy, quality of life scale (QLQ-C30, QLQ-LCL3) scores and immune indexes (CD4+, CD8+, CD4+/CD8+) were evaluated after two chemotherapy cycles. The comparison of clinical efficacy between the two groups yielded significant results (P >0.05). After treatment, physical, emotional, and fatigue scores in the comparison group increased significantly (P <0.05); shortness of breath in the research group decreased (P <0.05); loss of appetite in the comparison group rose (P<0.05); and total health in the research group increased significantly (P<0.05). ③ The research group’s CD4+, CD4+/CD8+ levels after treatment were greater than those in the comparison group, and CD8+ was lower (P <0.05). In vitro cultivation of cattle can enhance the clinical efficacy, quality of life, and immune function of those suffering from non-small cell lung cancer.