Light‐Driven Highly Selective Conversion of CO₂ to Formate by Electrosynthesized Enzyme/Cofactor Thin Film Electrode

Abstract

The highly selective electrochemical reduction of carbon dioxide (CO2) is reported to formate (HCOO‐) at a compactly integrated bioelectrode. The enzymatic biocatalytic cathode is fabricated by single‐step electropolymerization of a multifunctional polydopamine film in which enzyme/cofactor couples are uniquely embedded. Interestingly, this thin biohybrid system of nanoscale thickness assures unprecedentedly prolonged catalytic enzyme stability for about two weeks. Mimicking the natural photosynthesis, combination with the photoanode utilizing the water oxidation, steadily produces formate at a faradaic efficiency of 99.18 ± 6.77% with little degradation at least for 1 d under 1 sun illumination and no external bias.

Bioelectricity Generation by Corynebacterium glutamicum with Redox-Hydrogel-Modified Carbon Electrode

“…To enhance electrical communication between bacteria and an electrode, several polymer materials, such as nafion, polyamine, and polyaniline, have been used to fabricate anodes in MFCs [11,12]. Among them, chitosan, a linear aminopolysaccharide, has attracted interest for its unique chemical functionalities (polycationic and nucleophilic properties) that are suitable for building of a bio-electrode interface.…”

Recent Progress on Photo‐Electrocatalytic Reduction of Carbon Dioxide

“…The BiVO 4 /FeOOH photoanode provides a voltage compensation, lowering the total bias to drive the simultaneous water oxidation and CO 2 reduction. By integrating a BiVO 4 /CoPi photoanode with a biocathode, a bias‐free system successfully converting CO 2 into formate was achieved ( Figure a,b) . The biocathode, namely EC‐PDA, is composed of polydopamine (PDA) thin films copolymerized with an enzyme (E, FDH) and its cofactor (C, NADH) (Figure c).…”

“…c) Schematic illustration of 3D structure and proposed formation mechanism of EC‐PDA thin film‐coated biocathode. d) Photocatalytic formate formation from CO 2 gas as a function of irradiation time that performed using a two‐electrode configuration with no external bias in 0.1 m sodium phosphate (pH = 7.0) with the inset figure of chronoamperometry profile for 24 h. Reproduced with permission . Copyright 2016, Wiley‐VCH.…”

Polydopamine Based Colloidal Materials: Synthesis and Applications

“…These MIP materials were used as the capacitive sensors that showed an enantioselective recognition of glutamic acid with a good reproducibility, stability and repeatability . Recently, the other electrochemically synthesized polydopamine on electrodes was reported by Lee et al., which was used as a matrix for enzyme immobilization . As described, polydopamine films on the electrodes are helpful for facilitating electron transfer and stabilizing the enzyme for a prolonged lifetime.…”