Light effects on α-amylase activity and carbohydrate content in relation to lipid mobilization during the seedling growth of sunflower

Darbelley, Nicole; Razafindramboa, Njara; Chambost, Jean-Pierre; Pavia, A. A.

doi:10.1007/bf02524933

Cited by 7 publications

(5 citation statements)

References 38 publications

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“…4A) in the cotyledons coincides with limited accumulation of DW and starch in the hypocotyl (Figs 3A and 6A) and roots (Figs 3C and 7A) at 7 DAI. These results conflict with those previously found, in which the delay in lipid mobilisation is not accompanied by DW retention in the cotyledons, neither is it associated with DW decrease in the stem in sunflower seedlings exposed to darkness during the same period (Pfeiffer & Kutschera 1996;Darbelley et al 1997). These discrepancies might be due to the different genotypes utilised in the previous and current work.…”

Section: Discussioncontrasting

confidence: 99%

“…As expected, light-induced lipid mobilisation is accompanied by an increase in the activity of lipases, but activity of the glyoxysomal enzyme isocitrate lyase (ICL), implicated in the conversion of fatty acids into sucrose, is paradoxically reduced (Pfeiffer & Kutschera 1997). In addition, the accumulation of starch under continuous light is concomitant with a counterintuitive increase in activity of a-amylase in the cotyledons (Darbelley et al 1997).…”

Section: Introductionmentioning

confidence: 53%

“…), its accumulation in the different organs during post‐germination growth could be attributed to lipid mobilisation in the absence of photosynthesis, as previously described (Darbelley et al . ). Starch plays a central part as a transitory reserve, providing energy supply to heterotrophic or photosynthesising tissues in the absence of light (Orzechowski ).…”

Section: Discussionmentioning

confidence: 97%

“…In addition, the accumulation of starch under continuous light is concomitant with a counterintuitive increase in activity of α‐amylase in the cotyledons (Darbelley et al . ).…”

Section: Introductionmentioning

confidence: 97%

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Regulation of reserve mobilisation in sunflower during late seedling establishment in continuous darkness

et al. 2017

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Reserve mobilisation, metabolite partitioning and reserve-degrading enzyme activity were studied in sunflower seedlings cultivated in vitro under a 12-h photoperiod or in the dark to investigate the involvement of source-sink relation and carbon starvation in the regulation of reserve mobilisation under continuous darkness. Reserves, metabolites and enzyme activity were determined with standard spectrophotometric methods. At the first 24 h of treatment (acclimation phase), darkness did not affect growth, but restricted carbon and nitrogen use, as indicated by sugar and amino acid accumulation in the different seedling parts. After 5 days of treatment (survival phase), extended darkness limited growth and retarded storage lipid mobilisation due to carbon starvation, as evidenced by the depletion of carbohydrates in cotyledons and hypocotyl, as well as the consumption of amino acids in hypocotyls and roots. Alterations in the source-sink relationship might have been a response to prolonged darkness, instead of a mechanism used to regulate reserve mobilisation, as these alterations cannot be associated with negative feedback mediated by metabolite accumulation. Storage lipid degradation depends, at least in part, on mechanisms that co-ordinately regulate the activities of lipases and isocitrate lyase. Taking these results together, it is possible that reserve mobilisation in sunflower seedlings cultivated in the dark might be regulated by mechanisms that perceive the absence of light and predict carbon starvation, adjusting reserve use according to future energy demands to allow, at least in the short term, seedling survival.

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Section: Discussioncontrasting

confidence: 99%

Section: Introductionmentioning

confidence: 53%

Section: Discussionmentioning

confidence: 97%

“…In addition, the accumulation of starch under continuous light is concomitant with a counterintuitive increase in activity of α‐amylase in the cotyledons (Darbelley et al . ).…”

Section: Introductionmentioning

confidence: 97%

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Regulation of reserve mobilisation in sunflower during late seedling establishment in continuous darkness

et al. 2017

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“…Broeckling et al (2005) reported increases in the activities of some carbohydrate-related enzymes due to exposed of certain plant species to UVradiation. Furthermore, Darbelley et al (1997) investigated the changes in both invertase and α-amylase activities and in starch and free sugar contents in correlation with lipid mobilization in Helianthus annuus.…”

Section: Changes In Carbohydrates and Carbohydrate Related Enzymesmentioning

confidence: 99%

Comparative Effects of Stressful Factors on Growth and Carbohydrate Metabolism of Broad Bean Plants

Yoünis

Hasaneen

Kazamel

2012

Journal of Plant Production

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The effects of heat, UV-irradiation and salinity on growth and metabolism of broad bean plants were investigated. Exposure of bean plants to these stressful factors induced variable significant decreases in the levels of growth parameters throughout the experimental period, as compared with control plants. In relation to controls, direct exposure of broad bean plants to heat, UV-irradiation and salinity stresses, induced significant variable changes in the soluble carbohydrate components. Concurrently with carbohydrate changes, significant variable increases in the activities of both invertase and α-amylase of broad bean plants were maintained throughout the entire period of the experiment. 100 x 50 cm) in which UV radiation was supplied from a compact UV lamp, 254 nm and 365 nm (8 Wm-2) (UVP factory, USA) which was suspended above and perpendicular to the germination boxes at a distance of 100 cm. Also, after 16 days from sowing the seeds, the second group of pots was subdivided into three sets, one being left without treatment and the other two sets were subjected either to low (20 °C) or high (40 °C) temperatures. Treatment to either of the low or the high temperatures was carried out for 2 hours, every other day, for 10 days; pots being returned back to the original growth conditions, after each treatment. All the heat treatments were performed in growth chambers at controlled temperatures. The treatment scheme adopted can be summarized as follows: 1-Control. 2-Exposure of seedlings at low (20 °C) temperature. 3-Exposure of seedlings at high (40 °C) temperature. After treatment, the remaining seedlings were transplanted into large pots (30 cm in diameter) containing 9 kg clay: loamy soil; 2:1, v/v) and left to grow

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Seed reserve translocation and early seedling growth of eight tree species in a tropical deciduous forest in Mexico

et al. 2013

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Light effects on α-amylase activity and carbohydrate content in relation to lipid mobilization during the seedling growth of sunflower

Cited by 7 publications

References 38 publications

Regulation of reserve mobilisation in sunflower during late seedling establishment in continuous darkness

Regulation of reserve mobilisation in sunflower during late seedling establishment in continuous darkness

Comparative Effects of Stressful Factors on Growth and Carbohydrate Metabolism of Broad Bean Plants

Seed reserve translocation and early seedling growth of eight tree species in a tropical deciduous forest in Mexico

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