Light-emitting diode 585 nm photomodulation inhibiting melanin synthesis and inducing autophagy in human melanocytes

Chen, Li; Xu, Zhongyi; Jiang, Min; Zhang, Chengfeng; Wang, Xuan; Xiang, Leihong

doi:10.1016/j.jdermsci.2017.10.001

Cited by 34 publications

(47 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Upon undergoing UV exposure, melanocytes can undergo photosensitization by generating superoxide radicals in cells ( 97 ). In addition, p62 is upregulated upon phototherapy (UVA radiation and light-emitting ode 585 nm) in the melanocyte ( 61 , 62 ). Melanin confers protection from UV-induced DNA damage, maintains skin homeostasis, modulates the immune environment, and regulates skin color by autophagy ( 56 , 63 – 65 ).…”

Section: Autophagy In Skin Immune Cellsmentioning

confidence: 99%

More Than Skin Deep: Autophagy Is Vital for Skin Barrier Function

2018

View full text Add to dashboard Cite

The skin is a highly organized first line of defense that stretches up to 1.8 m2 and is home to more than a million commensal bacteria. The microenvironment of skin is driven by factors such as pH, temperature, moisture, sebum level, oxidative stress, diet, resident immune cells, and infectious exposure. The skin has a high turnover of cells as it continually bares itself to environmental stresses. Notwithstanding these limitations, it has devised strategies to adapt as a nutrient-scarce site. To perform its protective function efficiently, it relies on mechanisms to continuously remove dead cells without alarming the immune system, actively purging the dying/senescent cells by immunotolerant efferocytosis. Both canonical (starvation-induced, reactive oxygen species, stress, and environmental insults) and non-canonical (selective) autophagy in the skin have evolved to perform astute due-diligence and housekeeping in a quiescent fashion for survival, cellular functioning, homeostasis, and immune tolerance. The autophagic “homeostatic rheostat” works tirelessly to uphold the delicate balance in immunoregulation and tolerance. If this equilibrium is upset, the immune system can wreak havoc and initiate pathogenesis. Out of all the organs, the skin remains under-studied in the context of autophagy. Here, we touch upon some of the salient features of autophagy active in the skin.

show abstract

Section: Autophagy In Skin Immune Cellsmentioning

confidence: 99%

More Than Skin Deep: Autophagy Is Vital for Skin Barrier Function

2018

View full text Add to dashboard Cite

show abstract

“…Primary NHEK, NHEM, and dermal fibroblast cultures were performed as described previously (Chen et al, 2018) and were cultured under standard conditions in Medium 254 supplemented with human melanocyte growth factors (Invitrogen, Carlsbad, CA), keratinocyte-defined serum-free medium supplemented with human keratinocyte growth factors (Invitrogen), or DMEM (Invitrogen) medium supplemented with 10% fetal bovine serum (volume/volume). NHEKs, NHEMs, and human fibroblasts were fed every 2e3 days, and further passaged at 1:2 when they became 80% confluent, with experiments being carried out from 2 to 3 passages.…”

Section: Cell Culturementioning

confidence: 99%

CCN1/Cyr61 Stimulates Melanogenesis through Integrin α6β1, p38 MAPK, and ERK1/2 Signaling Pathways in Human Epidermal Melanocytes

Chen

Jiang

et al. 2018

Journal of Investigative Dermatology

Self Cite

View full text Add to dashboard Cite

Fibroblast-derived melanogenic paracrine mediators are known to play a role in melanogenesis. To investigate the effect of CCN1 (also called CYR61 or cysteine-rich 61) on melanogenesis, normal human epidermal melanocytes were treated with recombinant CCN1 protein. Our findings show that CCN1 activates melanogenesis through promoting melanosome maturation and up-regulation of MITF, TRP-1, and tyrosinase via the integrin α6β1, p38 MAPK, and ERK signaling pathways. Furthermore, we found that UVB irradiation stimulates the secretion of CCN1 from normal human dermal fibroblasts, and CCN1 knockdown in fibroblasts attenuates melanogenesis in melanocyte-fibroblast co-culture system. Moreover, using immunohistochemistry and immunofluorescence staining, we discovered that CCN1 is overexpressed in the dermis of both solar lentigines and Riehl's melanosis lesions. These findings suggest that CCN1 is a fibroblast-derived melanogenic paracrine mediator that is secreted under UVB irradiation, and it may play an important role in the development of hyperpigmentation diseases such as Riehl's melanosis.

show abstract

“…61 LED photo modulation at a 585 nm wavelength reduces the melanin content of inhuman epidermal melanocytes (HEMs), via dose-dependent inhibition of melanogenesis and induction of HEM autophagy. 62 It has been shown that β-mangostin from seedcases of Garcinia mangostana control α-MSH-mediated melanogenesis by inhibiting autophagy, which clearly recovers the premelanosome protein and tyrosinase degraded in B16F10 melanoma cells and a 3-dimensional human skin model. 2 Tranexamic acid (TXA) has been frequently used to decrease melanin synthesis in patients with melisma and as a raw material for functional whitening cosmetics.…”

Section: Current Mechanisms For Inhibiting Melanin Productionmentioning

confidence: 99%

Traditional Chinese Herbal Medicine for Whitening

Zhao

Wang

et al. 2020

Natural Product Communications

View full text Add to dashboard Cite

Melanin is the chief pigment responsible for the pigmentation of human skin. Increasing evidence indicates that traditional Chinese drugs with skin-whitening effects are attracting the attention of consumers and researchers because they are perceived to be milder, safer, and healthier than synthetic alternatives. This commentary summarizes the current research on Chinese herbal medicines that inhibit melanin and their biological activities. The findings presented in this study suggest that these traditional Chinese herbal medicines might be potential candidates for novel skin-whitening agents.

show abstract

Light-emitting diode 585 nm photomodulation inhibiting melanin synthesis and inducing autophagy in human melanocytes

Cited by 34 publications

References 38 publications

More Than Skin Deep: Autophagy Is Vital for Skin Barrier Function

More Than Skin Deep: Autophagy Is Vital for Skin Barrier Function

CCN1/Cyr61 Stimulates Melanogenesis through Integrin α6β1, p38 MAPK, and ERK1/2 Signaling Pathways in Human Epidermal Melanocytes

Traditional Chinese Herbal Medicine for Whitening

Contact Info

Product

Resources

About