Light-generated oligonucleotide arrays for rapid DNA sequence analysis.

Abstract

sequencing, termed sequencing by hybridization (SBH), has been proposed (1-3). This method uses a set of short oligonucleotide probes of defined sequence to search for complementary sequences on a longer target strand of DNA. The hybridization pattern is then used to reconstruct the target DNA sequence. It is envisioned that hybridization analysis of large numbers of probes can be used to sequence long stretches of DNA. In more immediate applications of hybridization methodology, a small number of probes can be used to interrogate local DNA structure.

A review on SNP and other types of molecular markers and their use in animal genetics

“…improvements of this family of techniques, is the use of DNA chips, on which the probes are directly synthesised using a parallel procedure involving masks and photolithography [62]. The densities thus obtained are extremely high and reliability is improved by using a tiled array scheme, multiplying the number of probes used for each base position questioned [29,80].…”

DNA microarray technologies for measuring protein–DNA interactions

“…DNA microarrays [1,2] together with whole-genome sequences have revolutionized mRNA expression analysis, and, more recently, they have facilitated biochemical and functional genomics studies of DNA binding proteins. Chromatin immunoprecipitation of a protein of interest followed by microarray-based detection of enriched DNA fragments, referred to as 'ChIP-chip' or 'genome-wide location analysis', is currently the most widely used method for identifying in vivo TF binding sites in a high-throughput manner [3][4][5][6].…”

Next generation microfluidic platforms for high-throughput protein biochemistry

“…The PCR product could also be purified in sufficient amounts to generate hundreds of DNA microarrays. Other advances to generating DNA microarrays included the photolithographic synthesis of oligos directly on the glass substrate at high spot densities [2,3]. For the detection of the mRNA sample, fluorescent nucleotides are incorporated into cDNA during a reverse transcription reaction.…”