Light Harvesting as a Simple and Versatile Way to Enhance Brightness of Luminescent Sensors

Mayr, Torsten; Borisov, Sergey M.; Abel, Tobias; Enko, Barbara; Waich, Kerstin; Mistlberger, Günter; Klimant, Ingo

doi:10.1021/ac900662x

Cited by 89 publications

(72 citation statements)

References 19 publications

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“…sigma aldrich.com) dissolved in a 4% wt wt −1 polystyrene matrix (www. goodfellow.com) using toluene as solvent (Mayr et al 2009, Larsen et al 2011. PtTFPP has excellent photostability and sensitivity in the lower concentration range (Borisov & Klimant 2007), and has a relatively high dynamic range and long-lived luminescence decay (Khalil et al 2005).…”

Section: Planar Optode Systemmentioning

confidence: 99%

Rhizosphere O2 dynamics in young Zostera marina and Ruppia maritima

Jovanovic¹,

Pedersen²,

Larsen

et al. 2015

Mar. Ecol. Prog. Ser.

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Zostera marina and Ruppia maritima often share the same habitat, but R. maritima appears more resistant to environmental stress. We investigated the impact of light intensity and water column O 2 concentrations on radial oxygen loss (ROL), in young specimens of Z. marina and R. maritima. Planar optode imaging revealed that ROL of Z. marina was localized to the root tip, while R. maritima showed ROL along extensive root sections. The total root biomass of the 2 species was similar, but, while R. maritima had only 1 root, of which 33% of its length showed ROL, Z. marina had 2 to 5 individual roots, where only 2 to 3 exhibited O 2 leakage, but then only at root tips. ROL resulted in an oxic volume of 4.26 ± 0.51 mm 3 plant −1 for Z. marina and 5.39 ± 0.47 mm 3 plant −1 for R. maritima (n = 3). ROL per plant at light saturation was 2.32 ± 0.30 and 2.89 ± 0.38 nmol h −1 for Z. marina and R. maritima, respectively. These values declined by 71 and 60% in darkness. However, both species were able to maintain ROL as long as ambient O 2 levels remained > 50% air saturation. The calculated ROL integrated over a 24 h cycle was 48.8 ± 10.6 nmol O 2 plant −1 d −1 (n = 3) for R. maritima and 30% less for Z. marina. The ability of R. maritima to maintain higher ROL than Z. marina could be an important feature defining its potential for colonizing and maintaining growth in eutrophic sediments.

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Section: Planar Optode Systemmentioning

confidence: 99%

Rhizosphere O2 dynamics in young Zostera marina and Ruppia maritima

Jovanovic¹,

Pedersen²,

Larsen

et al. 2015

Mar. Ecol. Prog. Ser.

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“…Sturdy fastresponding minielectrodes with outside diameters ranging from 0.1 to a few millimetres have been used for benthic investigations (Wenzhöfer et al 2000, de Beer et al 2005) and robust optodes have also proven useful for in situ operation in different benthic environments (Glud et al 1999, Wenzhöfer et al 2001). Novel, highly O 2 -sensitive chemiluminescent indicator complexes (Mayr et al 2009) have potential for a new generation of fast responding minioptodes that could provide a sturdy and cheap alternative to electrochemical sensors for eddy correlation O 2 measurements. …”

Section: Oxygen Exchange Rates As Derived From Eddy Correlationmentioning

confidence: 99%

Benthic O2 exchange across hard-bottom substrates quantified by eddy correlation in a sub-Arctic fjord

Glud¹,

Berg²,

Hume³

et al. 2010

Mar. Ecol. Prog. Ser.

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Oxygen exchange across the seabed is a key measure for quantifying benthic carbon turnover. However, technical limitations have restricted such measurements to sedimentary areas. Few assessments on hard-bottom substrates exist although such biotopes are frequent along many coastlines. In spring 2009, we used the non-invasive eddy correlation technique to quantify the in situ O 2 exchange rate across 3 typical substrates in West Greenland: (1) in soft sediments, the O 2 exchange in darkness averaged 2.0 mmol m -2 h -1. The rate exceeded the O 2 exchange quantified from microprofile measurements by 80%, but was not significantly different from parallel core incubations. (2) Measurements across consolidated sand and stones revealed a high activity with average O 2 exchange similar to that of sedimentary areas. Despite being net heterotrophic, the benthic primary production on average amounted to 27 mmol O 2 m -2 d -1, which per unit area is similar to the pelagic gross production of the central fjord. Carbon fixed by benthic diatoms and coralline red algae was efficiently recycled by microbes or grazed by the abundant epifauna. ). The O 2 exchange derived from the eddy correlation approach exhibited pronounced shortterm variability reflecting the interplay of several dynamic controls, most importantly short-term variations in local hydrodynamics. All together, measurements across hard-bottom substrates revealed active benthic communities that mediate high carbon turnover rates. Aquatic eddy correlation measurements show that hardbottom communities recycle large quantities of organic material and play an important role in coastal carbon cycling.

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“…Thinner layers are possible, if a stronger excitation light source is used or the imaged area is reduced (Kühl et al 2007). Other possibilities include the use of a more sensitive camera and the potential addition of light-harvesting molecules to the matrix (Mayr et al 2009). Using polymers with a higher molecular diffusion coefficient for oxygen would result in faster responding optodes, but lower spatial resolution.…”

Section: Assessment and Discussionmentioning

confidence: 99%

“…Since the sensing layer is coated directly onto a glass surface, there is no oxygen diffusion within the support foil or imaging window to be taken into account. Further improvements could be made by incorporating light-harvesting molecules in the sensing matrix to enhance the brightness of the image (Mayr et al 2009), which would allow the decrease of layer thickness to build faster sensors. Silanization of the FOFP before coating could be performed to optimize the adhesion of the sensing layer to the glass surface (Kühl et al 2007).…”

Section: Comments and Recommendationsmentioning

confidence: 99%

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2010

Limnology & Ocean Methods

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A novel high resolution planar optode (HiPO) for two dimensional oxygen and light-field imaging is presented. Optical cross-talk effects, limiting the precision and resolution of conventional planar optode setups, could be strongly reduced by the use of a fiber optic faceplate as sensing window. The new device, therefore, allows for accurate calculation of oxygen fluxes and respiration rates from oxygen concentration images, especially for measurements on small scales. In addition, the setup can be used to estimate the distribution of scalar irradiance within illuminated sediments or mats, thus directly linking local light availability to oxygen dynamics at high resolution. Irradiance values obtained with the HiPO were found to be in good agreement with scalar irradiance microsensor measurements in sandy sediment, and a spatial resolution of ~120 µm could be achieved. The performance of the HiPO for oxygen measurements was tested experimentally and the theoretical limit of spatial (~100 µm) and temporal (~10 s) resolution, governed by oxygen diffusion within the sensing layer, was determined by mathematical modeling. Rates of primary production and respiration in sandy sediments were calculated from the transient oxygen concentrations after perturbations in light condition showing a highly patchy distribution on submillimeter scale. These heterogeneities were clearly correlated to local irradiance within the sediment. Spatial correlations of photosynthesis and respiration were strongly dependent on incident irradiance.

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Light Harvesting as a Simple and Versatile Way to Enhance Brightness of Luminescent Sensors

Cited by 89 publications

References 19 publications

Rhizosphere O2 dynamics in young Zostera marina and Ruppia maritima

Rhizosphere O2 dynamics in young Zostera marina and Ruppia maritima

Benthic O2 exchange across hard-bottom substrates quantified by eddy correlation in a sub-Arctic fjord

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