Light-independent regulation of algal photoprotection by CO2 availability

Ruiz-Sola, M. Águila; Flori, Serena; Yuan, Yizhong; Villain, Gaëlle; Sanz-Luque, Emanuel; Redekop, Petra; Tokutsu, Ryutaro; Küken, Anika; Tsichla, Angeliki; Kepesidis, Georgios; Allorent, Guillaume; Arend, Marius; Iacono, Fabrizio; Finazzi, Guido; Hippler, Michael; Nikoloski, Zoran; Minagawa, Jun; Grossman, Arthur R.; Petroutsos, Dimitris

doi:10.21203/rs.3.rs-1886294/v1

Cited by 7 publications

(18 citation statements)

References 49 publications

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“…ROC75 and the CCM transporters LCIA and HLA3 are separated from the CCM cluster (Figure 1B). Taken together, the overall expression patterns in unbiased large-scale data reveal a diverse behaviour of the PP and CCM genes known to be co-expressed in some conditions (Ruiz-Sola et al, 2023;Arend et al, 2023). Co-expression with known TFs involved in these processes is limited, which indicates post-transcriptional modification, and/or non-linear, and/or combinatorial interactions.…”

Section: Resultsmentioning

confidence: 95%

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Multiple transcription factors mediate acclimation of Chlamydomonas to light stress

Wulf,

Krüger,

Klages

et al. 2023

Preprint

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Light as a substrate for photosynthesis may be a boon or a bane. To thrive, photosynthetic organisms must constantly respond to changing light and CO2 conditions by balancing energy harvest and consumption in a highly dynamic way. Two major safeguard measures of photoacclimation, that is photoprotection and carbon concentrating mechanism, underlie tight transcriptional control, leading to expression changes under high light and limited CO2 with different dynamics for both systems. Here, by using a consensus gene regulatory network inferred by employing a compendium of 1,869 RNA-seq datasets, we identified and validatedin vivoeight candidate transcription factors (TFs) that contribute to photoacclimation inChlamydomonas reinhardtii. Target gene analyses indicate that the TFs act individually in associated pathways but also influence each other in expression, and function as network parts with partial redundancy with respect to photoprotection. The analyses unveil that stress responses inChlamydomonasare mediated by a complex, interconnected network of TFs rather than a hierarchical system where multiple regulators can influence each other and target gene expression and thereby mitigate the effects of loss.

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Section: Resultsmentioning

confidence: 95%

“…Since TFs known to be involved in PP typically also mount a transcriptional phenotype for genes involved in the CCM (Ruiz-Sola et al, 2023;Arend et al, 2023), we tested the candidate TFs for changes in CCM gene expression (Figure 3) in response to low CO2 supply (Figure 3A, red line).…”

Section: Resultsmentioning

confidence: 99%

Multiple transcription factors mediate acclimation of Chlamydomonas to light stress

Wulf,

Krüger,

Klages

et al. 2023

Preprint

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“…Ionomic analysis was performed directly on snow water acidified with 0.65% (w/v) nitric acid and on snow water that has been concentrated 10 to 12.5 fold (freeze-drying followed by solubilization in 0.65% (w/v) nitric acid). Samples were analyzed using an iCAP RQ quadrupole mass instrument (Thermo Fisher Scientific GmbH, Germany) operating in the standard mode (for 11 B, 23 Na, 24 Mg, 25 Mg, 27 Al, 31 P, 39 K, 206 Pb, 208 Pb) and the collision mode with helium as a cell gas (for 31 P, 39 K, 44 Ca, 52 Cr, 53 Cr, 55 Mn, 56 Fe, 57 Fe, 59 Co, 63 Cu, 65 Cu, 64 Zn, 66 Zn, 69 Ga). Quantification was done using standard curves, corrected using the internal standards 103 Rh and 172 Yb, and molar concentrations were calculated using snow liquid water content of 15 mass%.…”

Section: Methodsmentioning

confidence: 99%

“…See supplementary Methods.Fluorescence-based measurements and statistical analysis. Fluorescence-based photosynthetic parameters were measured with a pulse modulated amplitude fluorimeter (MAXI-IMAGING-PAM, HeinzWaltz GmbH, Germany) as described in66 . Prior to the onset of the measurements, cells were acclimated to darkness for 15 min.…”

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confidence: 99%

Adaptive traits of cysts of the snow alga Sanguina nivaloides unveiled by 3D subcellular imaging

Maréchal

Ezzedine

Uwizeye

et al. 2023

Preprint

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Sanguina nivaloides is the main alga forming red snowfields in high mountains and Polar Regions. It is non-cultivable. Analysis of environmental samples by X-ray tomography, focused-ion-beam scanning-electron-microscopy, physicochemical and physiological characterization reveal adaptive traits accounting for algal capacity to reside in snow. Cysts populate liquid water at the periphery of ice, are photosynthetically active, can survive for months, and are sensitive to freezing. They harbour a wrinkled plasma membrane expanding the interface with environment. Ionomic analysis supports a cell efflux of K+, and assimilation of phosphorus. Glycerolipidomic analysis confirms a phosphate limitation. The chloroplast contains thylakoids oriented in all directions, fixes carbon in a central pyrenoid and produces starch in peripheral protuberances. Analysis of cells stored in the dark show that starch is a short-term carbon storage. The biogenesis of cytosolic droplets shows that they are loaded with triacylglycerol and carotenoids for long-term carbon storage and protection against oxidative stress.

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“…(HC) conditions (Ruiz-Sola et al 2023). However, both TpLhcx1/2 and TpLhcx6_1 proteins showed no response in accumulation level by changing the growth CO2 condition (Fig.…”

Section: Accumulation Of Tplhcx1/2 and Tplhcx6_1 Proteins At Differen...mentioning

confidence: 99%

Different functions of Lhcx isoforms in photoprotective mechanism in the marine diatomThalassiosira pseudonana

Nakayasu,

Akimoto,

Yoneda

et al. 2024

Preprint

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Photosynthesis needs light energy, but that exceeding the maximal capacity of photosynthesis enhances formation of reactive oxygen species, which potentially causes photodamages. Therefore, light-harvesting complexes (Lhc) in phototrophs harbor various proteins and pigments to function in both light capture and energy dissipation. Diatom Lhcx proteins are reported to be a critical component for thermal dissipation of excess light energy, but the molecular mechanism of photoprotection is still not fully understood and the functions of each Lhcx isoform are not yet differentiated. Here, we focused on two types of Lhcx isoforms in Thalassiosira pseudonana: TpLhcx1/2, putative major components for energy-dependent fluorescence quenching (qE); and TpLhcx6_1, functionally unknown isoform uniquely conserved in Thalassiosirales. TpLhcx1/2 proteins accumulated more under high light than under low light, while the TpLhcx6_1 protein level was constitutive irrespective of light intensities and CO2 concentrations. High-light induced photodamage of photosystem II was increased in the genome-editing transformants of these Lhcx isoforms relative to the wild-type. Transformants lacking TpLhcx1/2 showed significantly lowered qE capacities, strongly suggesting that these proteins are important for the fast thermal energy dissipation. While in contrast, genome-editing transformants lacking the TpLhcx6_1 protein rather increased the qE capacity. TpLhcx6_1 transformants were further evaluated by the low-temperature time-resolved chlorophyll fluorescence measurement, showing the longer fluorescence lifetime in transformants than that in the wild type cells even at the dark-acclimated state of these cells. These results suggest that TpLhcx6_1 functions in photoprotection through non-photochemical energy dissipation in the different way from qE.

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Light-independent regulation of algal photoprotection by CO2 availability

Cited by 7 publications

References 49 publications

Multiple transcription factors mediate acclimation of Chlamydomonas to light stress

Multiple transcription factors mediate acclimation of Chlamydomonas to light stress

Adaptive traits of cysts of the snow alga Sanguina nivaloides unveiled by 3D subcellular imaging

Different functions of Lhcx isoforms in photoprotective mechanism in the marine diatomThalassiosira pseudonana

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