Light-induced antibacterial activity of electrospun chitosan-based material containing photosensitizer

Severyukhina, A N; Petrova, N. V.; Yashchenok, Alexey M.; Bratashov, Daniil N.; Smuda, Kathrin; Mamonova, I. A.; Yurasov, Nikolay A.; Puchinyan, D M; Georgieva, Radostina; Bäumler, Hans; Lapanje, Aleš; Gorin, Dmitry A.

doi:10.1016/j.msec.2016.09.005

Cited by 36 publications

(20 citation statements)

References 48 publications

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“…From this, chitosan, which is biodegradable and non-toxic, can be derived and used for biomedical and pharmaceutical applications [ 113 , 119 ]. Chitosan can also be decorated with photosensitizers [ 120 , 121 ] and chitosan conjugated with rose bengal has been reported to enhance the degradation resistance of collagen than rose bengal alone, lacks residual activity, and is stable in the environment [ 115 , 118 ].…”

Section: Removal Of Endodontic Biofilms During Root Canal Treatmenmentioning

confidence: 99%

Biofilms in Endodontics—Current Status and Future Directions

Neelakantan

Romero

Vera

et al. 2017

IJMS

182

136

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Microbiota are found in highly organized and complex entities, known as biofilms, the characteristics of which are fundamentally different from microbes in planktonic suspensions. Root canal infections are biofilm mediated. The complexity and variability of the root canal system, together with the multi-species nature of biofilms, make disinfection of this system extremely challenging. Microbial persistence appears to be the most important factor for failure of root canal treatment and this could further have an impact on pain and quality of life. Biofilm removal is accomplished by a chemo-mechanical process, using specific instruments and disinfecting chemicals in the form of irrigants and/or intracanal medicaments. Endodontic research has focused on the characterization of root canal biofilms and the clinical methods to disrupt the biofilms in addition to achieving microbial killing. In this narrative review, we discuss the role of microbial biofilms in endodontics and review the literature on the role of root canal disinfectants and disinfectant-activating methods on biofilm removal.

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Section: Removal Of Endodontic Biofilms During Root Canal Treatmenmentioning

confidence: 99%

Biofilms in Endodontics—Current Status and Future Directions

Neelakantan

Romero

Vera

et al. 2017

IJMS

182

136

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“…Electrospinning has recently been shown to generate PAFs with significant antibacterial photodynamic effect against multiple bacteria [ 16 , 17 , 18 , 22 , 33 , 35 ]. Despite these initial advances, however, the biocompatibility of PAFs in both inert and antimicrobial states has only partially been addressed [ 24 , 33 , 35 ], which is critical to enable applicability in medical devices.…”

Section: Resultsmentioning

confidence: 99%

“…These limitations restrict the applicability of PDT in the clinical management of infectious diseases [ 13 ], at a time when the need for new antimicrobial strategies has never been more critical. Although the synthesis and testing of new PSs to address the above-mentioned challenges has been reported [ 10 , 11 , 14 ], encapsulation of PSs already in use in the clinic in polymer carriers represents a promising route to minimise PS aggregation, ensure a prolonged therapeutic window and comply with current regulatory framework [ 15 , 16 , 17 , 18 ]. With this strategy, control of the (i) release profile of PS from the carrier, (ii) PS uptake in both cells and bacteria and (iii) selectivity between the bactericidal effect and sparing effect upon host cells are key to successful performance.…”

Section: Introductionmentioning

confidence: 99%

Hydrolytic Degradability, Cell Tolerance and On-Demand Antibacterial Effect of Electrospun Photodynamically Active Fibres

et al. 2020

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Photodynamically active fibres (PAFs) are a novel class of stimulus-sensitive systems capable of triggering antibiotic-free antibacterial effect on-demand when exposed to light. Despite their relevance in infection control, however, the broad clinical applicability of PAFs has not yet been fully realised due to the limited control in fibrous microstructure, cell tolerance and antibacterial activity in the physiologic environment. We addressed this challenge by creating semicrystalline electrospun fibres with varying content of poly[(l-lactide)-co-(glycolide)] (PLGA), poly(ε-caprolactone) (PCL) and methylene blue (MB), whereby the effect of polymer morphology, fibre composition and photosensitiser (PS) uptake on wet state fibre behaviour and functions was studied. The presence of crystalline domains and PS–polymer secondary interactions proved key to accomplishing long-lasting fibrous microstructure, controlled mass loss and controlled MB release profiles (37 °C, pH 7.4, 8 weeks). PAFs with equivalent PLGA:PCL weight ratio successfully promoted attachment and proliferation of L929 cells over a 7-day culture with and without light activation, while triggering up to 2.5 and 4 log reduction in E. coli and S. mutans viability, respectively. These results support the therapeutic applicability of PAFs for frequently encountered bacterial infections, opening up new opportunities in photodynamic fibrous systems with integrated wound healing and infection control capabilities.

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“…PDT primarily uses various photosensitizers that are most active at inhibiting bacteria and other wound healing relevant microbes when activated by a light source, and this activation results in the molecule entering an excited state resulting in the evolution of ROS that disrupt microbial function . There is a truly impressive range of photosensitizers that are currently being investigated such as biopolymers that can be used in fabrics, nanoparticles amongst other materials and photoactive molecules . PDT‐based techniques have multiple advantages over traditional antibiotics such as speed of activity and lack of microbial resistance developed against them .…”

Section: Developments In Wound Treatmentmentioning

confidence: 99%

“…PDT‐based techniques have multiple advantages over traditional antibiotics such as speed of activity and lack of microbial resistance developed against them . Various photosensitizers have also been used with other technologies such as electrospinning and hydrogels, for topical application of antimicrobials . Even though PDT has been previously considered as primarily being used for cancer therapy, but due to the easy access of light to surface injuries such as cuts and burns, limited extra processes are required for the wound healing therapy, and this has already been demonstrated in in vivo testing .…”

Section: Developments In Wound Treatmentmentioning

confidence: 99%

Antimicrobial Peptide‐Based Electrospun Fibers for Wound Healing Applications

Dart

Bhave

Kingshott

2019

Macromolecular Bioscience

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Current wound healing treatments such as bandages and gauzes predominantly rely on passively protecting the wound and do not offer properties that increase the rate of wound healing. While these strategies are strong at protecting any infection after application, they are ineffective at treating an already infected wound or assisting in tissue regeneration. Next‐generation wound healing treatments are being developed at a rapid pace and have a variety of advantages over traditional treatments. Features such as gas exchange, moisture balance, active suppression of infection, and increased cell proliferation are all central to developing the next successful wound healing dressing. Electrospinning has already been shown to have the qualities required to be a key technique of next generation polymer‐based wound healing treatments. Combined with antimicrobial peptides (AMPs), electrospun dressings can indeed become a formidable solution for the treatment of both acute and chronic wounds. The literature on combining electrospinning and AMPs is now starting to increase and this review aims to give a comprehensive overview of the current developments that combine electrospinning technology and AMPs in order to make multifunctional fibers effective against infection in wound healing.

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Light-induced antibacterial activity of electrospun chitosan-based material containing photosensitizer

Cited by 36 publications

References 48 publications

Biofilms in Endodontics—Current Status and Future Directions

Biofilms in Endodontics—Current Status and Future Directions

Hydrolytic Degradability, Cell Tolerance and On-Demand Antibacterial Effect of Electrospun Photodynamically Active Fibres

Antimicrobial Peptide‐Based Electrospun Fibers for Wound Healing Applications

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