Light-induced effects in glycine aqueous solution studied by Fourier transform infrared-emission spectroscopy and ultraviolet-visible spectroscopy

Terpugov, E. L.; Kondratyev, Maxim S.; Degtyareva, O. V.

doi:10.1080/07391102.2020.1717628

Cited by 7 publications

(6 citation statements)

References 73 publications

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“…40 Even aged glycine solutions display higher absorbance compared to fresh glycine, suggesting that increased interaction of charged atoms with time in these glycine clusters leads to the generation of new species that can contribute to spectra. 55 Our results show that ProCharTS absorption intensity persists in monomeric proteins/peptides devoid of Lysine residues. The monomeric nature of the protein and peptides was confirmed by the linearly increasing ProCharTS absorbance with peptide/protein concentration, as shown in Fig.…”

Section: Discussionmentioning

confidence: 58%

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Protein charge transfer spectra in a monomeric protein with no lysine

Alom¹,

Swaminathan²

2023

Phys. Chem. Chem. Phys.

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Section: Discussionmentioning

confidence: 58%

Section: Discussionmentioning

confidence: 99%

Protein charge transfer spectra in a monomeric protein with no lysine

Alom¹,

Swaminathan²

2023

Phys. Chem. Chem. Phys.

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“…All FTIR spectra were recorded using an infrared spectrophotometer from 4,000 to 400/cm. The sample was scanned 64 times with a resolution of 4/cm (Terpugov et al., 2020; Lina et al., 2014).…”

Section: Methodsmentioning

confidence: 99%

Preparation of casein phosphopeptides calcium complex and the promotion in calcium cellular uptake through transcellular transport pathway

Duan

Yuan

et al. 2021

J Food Biochem

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This study evaluated the stability of casein phosphopeptides (CPP) and obtained peptide–calcium complex by heating and chelating the peptide with CaCl2 in a neutral solution. To assess the bioavailability of various calcium formulations, the calcium transport models were established in Caco‐2 cells, and the transcellular transport pathways of various calcium formulations were studied by RT‐PCR and Western blotting. Results of circular dichroism showed that CPP was a stable polypeptide. The ultraviolet absorption spectrum and Fourier transform‐infrared spectrum (FT‐IR) indicated that calcium could be chelated by carboxyl oxygen and amino nitrogen atoms of CPP to form peptide calcium chelate, and the calcium bioavailability of peptide calcium chelate was significantly higher than that of CaCl2, calcium l‐aspartate, and casein phosphopeptides mixed with CaCl2. Four calcium sources increased the expression of TRPV5 and TRPV6 genes and proteins. The study intended to provide a basis for developing a novel calcium supplement. Practical applications This paper examined the bioavailability of casein phosphopeptides calcium complex, CaCl2, calcium l‐aspartate, and casein phosphopeptides mixed with CaCl2 in Caco‐2 cells, and the mechanisms were detected by western blotting. The results provide theoretical knowledge for the selection of calcium supplement raw materials and lay a foundation for the development of compound calcium preparations and drugs in the future.

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“…It is generally accepted that molecular glycine does not absorb light in the near UV/vis range, similar to other amino acids (Bhat and Dharmaprakash, 2002). However, it has been shown that their optical properties change when glycine molecules arrange themselves into mesoscopic clusters (Terpugov et al, 2021). Furthermore, these formations respond to light irradiation in non-trivial ways which can be exploited to induce long range order inside the glycine solution (Alexander and Camp, 2019;Sugiyama et al, 2012;Zaccaro et al, 2001;Garetz et al, 2002) on a scale of up to millimeters (Yuyama et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

“…leading to an enhancement of the absorption and fluorescence in the case of protein aggregates (Homchaudhuri and Swaminathan, 2004;Shukla et al, 2004;Chan et al, 2013;Pinotsi et al, 2013) and amino acid clusters (Chen et al, 2018). For glycine solutions in particular, observations of light absorption in the near UV/vis range have been attributed to the presence of mesoscopic clusters (Jawor-Baczynska et al, 2013;Zimbitas et al, 2019), specifically due to the formation of hydrogen bonds between individual molecules (Terpugov et al, 2021).…”

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confidence: 99%

Photo-Induced Shrinking of Aqueous Glycine Aerosol Droplets

Ishizuka

Reich

David

et al. 2023

Preprint

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Abstract. Due to their small size, micrometer and submicron sized solution droplets can respond differently to physical and chemical processes compared with extended bulk material. Using optically trapped micrometer sized aqueous glycine droplets, we demonstrate photo-induced degradation of glycine upon irradiation with visible light, even though molecular glycine does not absorb light in the near UV/vis range to any significant extent. This reaction is observed as photo-induced shrinking of the droplet, which we characterize by analyzing the elastic light scat-tering and the Raman spectrum of the droplet over the course of the reaction. We find the volume to shrink with a constant rate over the major part of the shrinking process. This indicates the presence of a rate limiting photo-catalyst, which we attribute to mesoscopic glycine clusters in the droplet solution. Our findings relate to previous reports of visible light absorption by photosensitizers. However, to the best of our knowledge, this is the first experimental evidence of a photochemical pathway facilitated by mesoscopic clusters. Light interaction with such mesoscopic photoactive molecular aggregates might be more important for aerosol photochemistry than previously anticipated.

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Light-induced effects in glycine aqueous solution studied by Fourier transform infrared-emission spectroscopy and ultraviolet-visible spectroscopy

Cited by 7 publications

References 73 publications

Protein charge transfer spectra in a monomeric protein with no lysine

Protein charge transfer spectra in a monomeric protein with no lysine

Preparation of casein phosphopeptides calcium complex and the promotion in calcium cellular uptake through transcellular transport pathway

Photo-Induced Shrinking of Aqueous Glycine Aerosol Droplets

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