Light induces expression of a dehydrin-encoding gene during seedling de-etiolation in sunflower (Helianthus annuus L.)

Natali, Lucia; Giordani, Tommaso; Lercari, Bartolomeo; Maestrini, P; Cozza, Radiana; Pangaro, T.; Vernieri, Paolo; Martinelli, Federico; Cavallini, Andrea

doi:10.1016/j.jplph.2006.01.015

Cited by 21 publications

(13 citation statements)

References 43 publications

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“…According to observations of Rorat and co-workers (2004) in five Solanaceae species and barley, leaves of young, developing seedlings exhibited high dehydrin protein levels even in the absence of stress. Similarly, light induced expression of a dehydrin-encoding gene, HaDhn1, was found in sunflower seedlings in the absence of environmental stresses (Natali et al, 2007). This made us speculate that wheat seedlings may also express at least some DHN and/or Cbf genes spontaneously (i.e.…”

Section: Droughmentioning

confidence: 80%

C-Repeat Binding Factor and Dehydrin Genes are Induced Co-Ordinately in Drought Tolerance Response of Wheat Cultivars

Deák¹,

Jäger²,

Nagy³

et al. 2017

JAS

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Four bread wheat genotypes with contrasting drought stress tolerance were studied. Expression levels of dehydrin (Wdhn13) and C-repeat binding factor (Cbf14, Cbf15) genes were investigated in leaves of two drought tolerant (Plainsman V, Mv Emese) and two sensitive (GK Élet, Cappelle Desprez) cultivars by semi-quantitative RT-PCR during drought treatment at anthesis. Coordinate induction of Cbf14, Cbf15 and Wdhn13 genes occurred at a late stage of stress treatment in all cultivars except the most sensitive Cappelle Desprez, where no induction was evident. The most pronounced late induction of genes was observed in the tolerant Mv Emese genotype. Cbf14, Cbf15 and Wdhn13 showed largely parallel changes of expression in stressed adult plants. The mRNA level of the same set of genes was measured in leaves of non-stressed seedlings with qRT-PCR method. Expression level of Wdhn13 was high and low in seedlings of tolerant and sensitive cultivars, respectively. Cbf15 specific transcript was barely detectable in leaves of non-stressed seedlings. In order to shed light on any potential difference in hormone responsiveness, seedlings were subjected to ABA treatment in vitro. At low hormone concentrations (10 and 20 µM ABA) consistently weaker ABA induced root growth retardation of GK Élet was found in comparison with the other three cultivars. Results highlight pronounced and late induction of a set of defence genes and low ABA sensitivity as features appearing in drought tolerant and sensitive responses, respectively. Data is discussed in the light of multifactorial determination of the complex phenotype of drought tolerance in wheat.

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Section: Droughmentioning

confidence: 80%

C-Repeat Binding Factor and Dehydrin Genes are Induced Co-Ordinately in Drought Tolerance Response of Wheat Cultivars

Deák¹,

Jäger²,

Nagy³

et al. 2017

JAS

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“…Metabolomics, largely used for the elucidation of plant physiological processes (Tosetti et al, 2012;Martinelli et al, 2011a;2012b;Ibanez et al, 2014) is another potent tool for the identification of metabolic biomarkers for early detection of diseases. Genes involved in phenylpropanoid pathways or chaperones Natali et al, 2007) may be potential targets to characterize plant stress status.…”

Section: Detection Methods Of Plant Diseasesmentioning

confidence: 99%

Proposal of a Genome Editing System for Genetic Resistance to Tomato Spotted Wilt Virus

Martinelli¹,

Grillone²,

Sgroi³

2014

American Journal of Applied Sciences

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Viruses provoke considerable economical losses in agriculture. New molecular approaches to develop genetic resistance based on translational genomics and precision genetic modifications are highly expected. The type II Clustered, Regularly Interspaced Palindromic Repeats (CRISPR) system including Cas9 nuclease represent a promising and very powerful tool to specifically modulate the expression and activity of genes involved in biotic stress responses. In this study, we describe an approach to develop a platform system based on CRISPR system for genome editing technology in tomato. Tomato is an excellent plant for this approach considering the high-quality genome sequence, the rapid life cycle, the highly efficient in vitro plant culture and transformation protocols, Genome editing can be used to allow resistance to Tomato Spotted Wilt Virus (TSWV) infections by the successful obtainment of two specific objectives: (1) development of a Genome Editing (GE) system using CRISPR-Cas9 system in tomato (Objective 1) and (2) test the system in inducing genetic resistance to TSWV infections. First, it will be necessary to model the molecular dynamics of key host and pathogen proteins predicting how targeted mutations affect these interactions. Then these host players will be targeted by CRISPR-Cas9 technology. The obtained plants can be evaluated for their phenotypic resistance and deeply analyzed using "omic" platforms to gain insight into gene regulatory networks of plant resistance. Outcomes of the proposed project will be essentially three: (1) Identify host target proteins interacting with pathogenic proteins and model their dynamic interactions; (2) develop a platform technology usable to obtain resistant tomatoes to TSWV inducing targeted genetic modifications in the genome; (3) facilitate the adaption of this platform to the improvement of important traits in other specialty crops.

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“…Of albedo proteins encoded by genes showing significant differential expression between puffed and control samples, heat shock proteins HSP82 and HSC70, farnesyl diphosphate synthase 2 (FPS2), rab GTPase homolog (RABH1), and ATRAD51 had the highest number of protein-protein interactions. However, it is also possible that genes with similar function to heat shock proteins could be induced in the absence of stresses [56]. Several hub proteins were regulated in both albedo and flavedo: HSP81-1, ubiquitin binding protein (AT-MCB1), cell division control 2 (CDC2), albino pale green 6 (APG6), and sugar transport protein 13 (MSS1).…”

Section: Protein-protein Interaction Network Analysismentioning

confidence: 99%

Transcriptome and metabolome analysis of Citrus fruit to elucidate puffing disorder

et al. 2014

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a b s t r a c tA systems-level analysis reveals details of molecular mechanisms underlying puffing disorder in Citrus fruit. Flavedo, albedo and juice sac tissues of normal fruits and fruits displaying symptoms of puffing disorder were studied using metabolomics at three developmental stages. Microarrays were used to compare normal and puffed fruits for each of the three tissues. A protein-protein interaction network inferred from previous work on Arabidopsis identified hub proteins whose transcripts show significant changes in expression. Glycolysis, the backbone of primary metabolism, appeared to be severely affected by the disorder, based on both transcriptomic and metabolomic results. Significantly less citric acid was observed consistently in puffed fruits. Gene set enrichment analysis suggested that glycolysis and carbohydrate metabolism were significantly altered in puffed samples in both albedo and flavedo. Expression of invertases and genes for sucrose export, amylose-starch and starch-maltose conversion was higher in puffed fruits. These changes may significantly alter source-sink communications. Genes associated with gibberellin and cytokinin signaling were downregulated in symptomatic albedo tissues, suggesting that these hormones play key roles in the disorder. Findings may be applied toward the development of early diagnostic methods based on host response genes and metabolites (i.e. citric acid), and toward therapeutics based on hormones.

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Light induces expression of a dehydrin-encoding gene during seedling de-etiolation in sunflower (Helianthus annuus L.)

Cited by 21 publications

References 43 publications

C-Repeat Binding Factor and Dehydrin Genes are Induced Co-Ordinately in Drought Tolerance Response of Wheat Cultivars

C-Repeat Binding Factor and Dehydrin Genes are Induced Co-Ordinately in Drought Tolerance Response of Wheat Cultivars

Proposal of a Genome Editing System for Genetic Resistance to Tomato Spotted Wilt Virus

Transcriptome and metabolome analysis of Citrus fruit to elucidate puffing disorder

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