Light Intensity Adaptation and Phycobilisome Composition of <i>Microcystis aeruginosa</i>

Raps, Shirley; Kycia, J. Helen; Ledbetter, Myron C.; Siegelman, H. W.

doi:10.1104/pp.79.4.983

Cited by 48 publications

(36 citation statements)

References 23 publications

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“…This effect might be related to nitrogen limitation as cell abundance increases [65]; however, nutrient data acquired simultaneously reveal no shortage of total nitrogen (or other nutrients) associated with reduced PC:chl-a ratios ( Figure 17C). An alternative explanation is that phycobilipigment production is reduced in high light, shallow mixed surface scum layers [63,66] or in fast growth scenarios. While a precise explanation for this effect remains somewhat unknown, the observation has some important implications for extremely high biomass and surface scum scenarios.…”

Section: Tchl-specific Phytoplankton Absorptionmentioning

confidence: 99%

Characterizing the Absorption Properties for Remote Sensing of Three Small Optically-Diverse South African Reservoirs

Matthews

Bernard

2013

Remote Sensing

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Characterizing the specific inherent optical properties (SIOPs) of water constituents is fundamental to remote sensing applications.Therefore, this paper presents the absorption properties of phytoplankton, gelbstoff and tripton for three small, optically-diverse South African inland waters. The three reservoirs, Hartbeespoort, Loskop and Theewaterskloof, are challenging for remote sensing, due to differences in phytoplankton assemblage and the considerable range of constituent concentrations. Relationships between the absorption properties and biogeophysical parameters, chlorophyll-a (chl-a), TChl (chl-a plus phaeopigments), seston, minerals and tripton, are established. The value determined for the mass-specific tripton absorption coefficient at 442 nm, a * tr (442), ranges from 0.024 to 0.263 m 2 ·g −1 . The value of the TChl-specific phytoplankton absorption coefficient (a * φ ) was strongly influenced by phytoplankton species, size, accessory pigmentation and biomass. a * φ (440) ranged from 0.056 to 0.018 m 2 ·mg −1 in oligotrophic to hypertrophic waters. The positive relationship between cell size and trophic state observed in open ocean waters was violated by significant small cyanobacterial populations. The phycocyanin-specific phytoplankton absorption at 620 nm, a * pc (620), was determined as 0.007 m 2 ·g −1 in a M. aeruginosa bloom. Chl-a was a better indicator of phytoplankton biomass than phycocyanin (PC) in surface scums, due to reduced accessory pigment production. Absorption budgets demonstrate that monospecific blooms of M. aeruginosa and C. hirundinella may be treated as "cultures", removing some complexities for remote sensing applications. These results contribute toward a better Remote Sens. 2013, 5 4371 understanding of IOPs and remote sensing applications in hypertrophic inland waters. However, the majority of the water is optically complex, requiring the usage of all the SIOPs derived here for remote sensing applications. The SIOPs may be used for developing remote sensing algorithms for the detection of biogeophysical parameters, including chl-a, suspended matter, tripton and gelbstoff, and in advanced remote sensing studies for phytoplankton type detection.

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Section: Tchl-specific Phytoplankton Absorptionmentioning

confidence: 99%

Characterizing the Absorption Properties for Remote Sensing of Three Small Optically-Diverse South African Reservoirs

Matthews

Bernard

2013

Remote Sensing

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“…Light intensity can modify both PBP content and PBS number. For example, in cyanobacteria such as Microcystis aeruginosa, HL results in a decrease in the number of PBS per cell, with their composition and structure remaining unchanged (Raps et al, 1985), whereas in Synechococcus sp.…”

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confidence: 99%

Evidence for a Transient Association of New Proteins with the Spirulina maxima Phycobilisome in Relation to Light Intensity

1994

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Environmental parameters are known to affect phycobilisomes. Variations of their structure and relative composition in phycobiliproteins have been observed. We studied the effect of irradiance variations on the phycobilisome structure in the cyanobacterium Spirulina maxima and discovered the appearance of new polypeptides associated with the phycobilisomes under an increased light intensity. In high light, the six rods of phycocyanin associated with the central core of allophycocyanin contained only one to two phycocyanin hexamers instead of the two to three they contained in low light. The concomitant disappearance of a 33-kD linker polypeptide was observed. Moreover, in high light three polypeptides of 29, 30, and 47 kD, clearly unrelated to linkers, were found to be associated with the phycobilisome fraction: protein labeling showed that a specific association of these polypeptides was induced by high light. One polypeptide, at least, would play the role of a chaperone protein. Not only the synthesis of these proteins, which appeared slightly increased in high light, but also their association with phycobilisome structure are light intensity dependent.

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“…Since there are two copies of L CM per PBS structure, each APC trimeric cylinder could potentially be detached, leading to a complete dissociation of PBS from the thylakoid membrane. Thus, one would expect that degradation of L CM should lead to a decrease in the whole-cell content in assembled PBS, an observation which was indeed reported for some cyanobacteria during light acclimation (30).…”

Section: Discussionmentioning

confidence: 95%

“…The amount and composition of PBS are modified with light conditions and nutrient availability (2,6,16,17). Acclimation to higher light intensity occurs primarily through changes in gene expression (4,18,22) that result in a decreased number of PBS per cell and in a shortening of PBS rods (18,30). Other well-studied examples of acclimation are the degradation of PBS during nitrogen, phosphor, and sulfur starvations (29,41).…”

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Involvement of the SppA1 Peptidase in Acclimation to Saturating Light Intensities in Synechocystis sp. Strain PCC 6803

et al. 2004

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The sll1703 gene, encoding an Arabidopsis homologue of the thylakoid membrane-associated SppA peptidase, was inactivated by interposon mutagenesis in Synechocystis sp. strain PCC 6803. Upon acclimation from a light intensity of 50 to 150 E m ؊2 s ؊1 , the mutant preserved most of its phycobilisome content, whereas the wild-type strain developed a bleaching phenotype due to the loss of about 40% of its phycobiliproteins. Using in vivo and in vitro experiments, we demonstrate that the ⌬sppA1 strain does not undergo the cleavage of the L R 33 and L CM 99 linker proteins that develops in the wild type exposed to increasing light intensities. We conclude that a major contribution to light acclimation under a moderate light regime in cyanobacteria originates from an SppA1-mediated cleavage of phycobilisome linker proteins. Together with changes in gene expression of the major phycobiliproteins, it contributes an additional mechanism aimed at reducing the content in phycobilisome antennae upon acclimation to a higher light intensity.

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Light Intensity Adaptation and Phycobilisome Composition of Microcystis aeruginosa

Cited by 48 publications

References 23 publications

Characterizing the Absorption Properties for Remote Sensing of Three Small Optically-Diverse South African Reservoirs

Characterizing the Absorption Properties for Remote Sensing of Three Small Optically-Diverse South African Reservoirs

Evidence for a Transient Association of New Proteins with the Spirulina maxima Phycobilisome in Relation to Light Intensity

Involvement of the SppA1 Peptidase in Acclimation to Saturating Light Intensities in Synechocystis sp. Strain PCC 6803

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