Light microscopy, with a history of over 400 years, continues to be useful for the natural sciences. It has played a crucial role in the physical and biological sciences, from providing evidence for the existence of atoms to resolving cells at very high resolution. In this article, we have taken a broad view of light microscopy, in its various forms to provide physical information that helps understand the structure and perhaps, the function of, certain materials, in particular polymers, liquid crystals, and beetle exocuticles, among others. We illustrate the information that can be gleaned by polarized light microscopy studies of semicrystalline polymers and liquid crystals. We also demonstrate that polarized light microscopy is an indispensable tool in studying anisotropic structures. We illustrate how optical sectioning microscopy, namely laser scanning confocal microscopy, is useful in studying diffusion as well as analyzing the structure of jewel beetle exocuticles, and providing the elusive director configuration in three‐dimension for a liquid crystal. Finally, we demonstrate a technique based on total internal reflection to probe the topography of polymeric surfaces. In doing so, we have illustrated the rich history of light microscopy and the elegance of Ernst Abbe's work dealing with the resolution that is attainable by far‐field light microscopy.