Light-patterning of synthetic tissues with single droplet resolution

Booth, Michael J.; Schild, Vanessa Restrepo; Box, Stuart J.; Bayley, Hagan

doi:10.1038/s41598-017-09394-9

Cited by 68 publications

(70 citation statements)

References 38 publications

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“…At the simplest level, this allows the flow of water due to gradients in osmotic pressure (leading to macroscopic evolution in the structure) and the conduction of ionic currents (Figure d,e). More complex implementations have since harnessed this capability to perform complex, multistep chemical reactions is compartmentalized systems . The encapsulation of living matter within these systems promises to add further complexity to the system, allowing for selective and spatially organized chemical communication between communities of microorganisms …”

Section: Reconfigurable Time‐evolving All‐liquid Materialsmentioning

confidence: 99%

Building Reconfigurable Devices Using Complex Liquid–Fluid Interfaces

et al. 2019

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imparts novel mechanical and functional properties to the macroscopic interface itself. These properties include size-and charge-selective pathways for molecules and particulates, shear and compressive moduli, and selective binding and reaction sites. Complex interfaces can then be used to generate complex, macroscopic, all-liquid materials with very high surface areas that have unique properties, such as compartmentalization, communication between compartments, and the ability to move and reconfigure. With the rapid growth in the study of complex materials made from interfacially structured liquids, there is a need to review the field from the funda-Liquid-fluid interfaces provide a platform both for structuring liquids into complex shapes and assembling dimensionally confined, functional nanomaterials. Historically, attention in this area has focused on simple emulsions and foams, in which surface-active materials such as surfactants or colloids stabilize structures against coalescence and alter the mechanical properties of the interface. In recent decades, however, a growing body of work has begun to demonstrate the full potential of the assembly of nanomaterials at liquid-fluid interfaces to generate functionally advanced, biomimetic systems. Here, a broad overview is given, from fundamentals to applications, of the use of liquid-fluid interfaces to generate complex, all-liquid devices with a myriad of potential applications. Figure 2.Interactions between particles attached to liquid-fluid interfaces. a) Dipole-dipole interactions between adsorbed particles due to asymmetric charge distributions near the surface of particles at interfaces. Reproduced with permission. [34] Copyright 1980, American Physical Society. b) Dipole-dipole interactions give rise to 2D colloidal crystals with large lattice spacings. Scale bar, 50 µm. Reproduced with permission. [36]

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Section: Reconfigurable Time‐evolving All‐liquid Materialsmentioning

confidence: 99%

Building Reconfigurable Devices Using Complex Liquid–Fluid Interfaces

et al. 2019

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“…Furthermore, cooperative/consolidated performance, a prerequisite of generating the emergent characteristics of higher‐order entities such as whole organism, organs, and tissues, could be obtained through communication and cooperation. Such potential can only be conceived if the single synthetic cells are induced to respond to particular signals while acting as building blocks and working as one simultaneously (Booth, Restrepo Schild, Box, & Bayley, 2017). In this line, design of a tissue‐like printed material, capable of mimicking engineered tissues is demonstrated through 3D printing, where printed droplet networks can be applied as living tissues mimics, or as a tissue engineering material.…”

Section: Cfps: Latest Platforms and Applications In Biotechnology Andmentioning

confidence: 99%

Cell‐free protein synthesis: The transition from batch reactions to minimal cells and microfluidic devices

Ayoubi‐Joshaghani

Dianat‐Moghadam

Seidi

et al. 2020

Biotech & Bioengineering

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Thanks to the synthetic biology, the laborious and restrictive procedure for producing a target protein in living microorganisms by biotechnological approaches can now experience a robust, pliant yet efficient alternative. The new system combined with lab‐on‐chip microfluidic devices and nanotechnology offers a tremendous potential envisioning novel cell‐free formats such as DNA brushes, hydrogels, vesicular particles, droplets, as well as solid surfaces. Acting as robust microreactors/microcompartments/minimal cells, the new platforms can be tuned to perform various tasks in a parallel and integrated manner encompassing gene expression, protein synthesis, purification, detection, and finally enabling cell‐cell signaling to bring a collective cell behavior, such as directing differentiation process, characteristics of higher order entities, and beyond. In this review, we issue an update on recent cell‐free protein synthesis (CFPS) formats. Furthermore, the latest advances and applications of CFPS for synthetic biology and biotechnology are highlighted. In the end, contemporary challenges and future opportunities of CFPS systems are discussed.

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“…In recent years, the fabrication of artificial biomimetic materials has attracted a great deal of attention . Attributed for the development of materials science and engineering science, various artificial organs, such as heart, blood vessels, liver acinus, skin, muscle, and nerve, have been fabricated. Besides artificial organs, the fabrication of artificial cells has aroused a great deal of attention as well.…”

Section: Introductionmentioning

confidence: 99%

Microfluidics for Biosynthesizing: from Droplets and Vesicles to Artificial Cells

Xie

Xiong

et al. 2019

Small

121

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Fabrication of artificial biomimetic materials has attracted abundant attention. As one of the subcategories of biomimetic materials, artificial cells are highly significant for multiple disciplines and their synthesis has been intensively pursued. In order to manufacture robust “alive” artificial cells with high throughput, easy operation, and precise control, flexible microfluidic techniques are widely utilized. Herein, recent advances in microfluidic‐based methods for the synthesis of droplets, vesicles, and artificial cells are summarized. First, the advances of droplet fabrication and manipulation on the T‐junction, flow‐focusing, and coflowing microfluidic devices are discussed. Then, the formation of unicompartmental and multicompartmental vesicles based on microfluidics are summarized. Furthermore, the engineering of droplet‐based and vesicle‐based artificial cells by microfluidics is also reviewed. Moreover, the artificial cells applied for imitating cell behavior and acting as bioreactors for synthetic biology are highlighted. Finally, the current challenges and future trends in microfluidic‐based artificial cells are discussed. This review should be helpful for researchers in the fields of microfluidics, biomaterial fabrication, and synthetic biology.

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Light-patterning of synthetic tissues with single droplet resolution

Cited by 68 publications

References 38 publications

Building Reconfigurable Devices Using Complex Liquid–Fluid Interfaces

Building Reconfigurable Devices Using Complex Liquid–Fluid Interfaces

Cell‐free protein synthesis: The transition from batch reactions to minimal cells and microfluidic devices

Microfluidics for Biosynthesizing: from Droplets and Vesicles to Artificial Cells

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