Light penetration into cell suspensions of photosynthetic bacteria and relation to hydrogen production

Nakada, Eiju; Asada, Yasuo; Arai, Takaaki; Miyake, Jun

doi:10.1016/0922-338x(95)98176-l

Cited by 105 publications

(56 citation statements)

References 3 publications

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“…To operate within these constraints a PBR volume of 7648 L would be required. The corresponding square panel PBR could be 12.4 m wide with a light capture area of 153.0 m 2 and a depth of 5 cm; sufficiently shallow to maintain the entire culture under saturating light intensity (Nakada et al 1995;Katsuda et al 2000). This area could potentially harvest 6.6 GJ per 12 h light period, indicating a comfortable light conversion efficiency of 1.65 % to meet the H 2 demand (Figure 4).…”

Section: Energy Generation Potential With a Dark Fermentative 1 St Stmentioning

confidence: 99%

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Integrating dark and light bio-hydrogen production strategies: towards the hydrogen economy

Redwood

Paterson-Beedle

Macaskie

2008

Rev Environ Sci Biotechnol

140

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Biological methods of hydrogen production are preferable to chemical methods because of the possibility to use sunlight, CO 2 and organic wastes as substrates for environmentally benign conversions, under moderate conditions. By combining different microorganisms with different capabilities, the individual strengths of each may be exploited and their weaknesses overcome. Mechanisms of bio-hydrogen production are described and strategies for their integration are discussed. Dual systems can be divided broadly into wholly light-driven systems (with microalgae/cyanobacteria as the 1 st stage) and partially light-driven systems (with a dark, fermentative initial reaction). Review and evaluation of published data suggests that the latter type of system holds greater promise for industrial application. This is because the calculated land area required for a wholly light-driven dual system would be too large for either centralised (macro-) or decentralised (micro-)energy generation. The potential contribution to the hydrogen economy of partially light-driven dual systems is overviewed alongside that of other bio-fuels such as bio-methane and bio-ethanol.Redwood et al. -Dual systems for Bio-H 2 -Reviews in Environ. Sci. Bio/Technol. 8:149 http://dx

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Section: Energy Generation Potential With a Dark Fermentative 1 St Stmentioning

confidence: 99%

“…Purple non-sulfur (PNS) bacteria are anoxygenic photosynthetic bacteria which, unlike the purple and green sulfur bacteria, do not produce H 2 S (a powerful catalyst poison) and the off-gas is typically > 90 % H 2 , hence it is suitable for use in PEM-fuel cells without purification (Nakada et al 1995).…”

Section: 12: Photoheterotrophsmentioning

confidence: 99%

Integrating dark and light bio-hydrogen production strategies: towards the hydrogen economy

Redwood

Paterson-Beedle

Macaskie

2008

Rev Environ Sci Biotechnol

140

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“…Such adjustments of the Chl antenna size in response to irradiance are a guided compensation reaction of the chloroplast to prevent over-excitation of the photosystems and potential photo-oxidative damage [4]. This molecular regulatory mechanism for the adjustment and optimization of the Chl antenna is highly conserved and functions in all organisms with oxygenic and anoxygenic photosynthesis [9,12,17,18]. Accordingly, there are nuclear and plastid genes that define the size and/or regulate the Chl antenna size of photosynthetic organisms [19,20].…”

Section: Introductionmentioning

confidence: 99%

Modulation of the light-harvesting chlorophyll antenna size in Chlamydomonas reinhardtii by TLA1 gene over-expression and RNA interference

Mitra

Kirst

Dewez

et al. 2012

Phil. Trans. R. Soc. B

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Truncated light-harvesting antenna 1 (TLA1) is a nuclear gene proposed to regulate the chlorophyll (Chl) antenna size in Chlamydomonas reinhardtii. The Chl antenna size of the photosystems and the chloroplast ultrastructure were manipulated upon TLA1 gene over-expression and RNAi downregulation. The TLA1 over-expressing lines possessed a larger chlorophyll antenna size for both photosystems and contained greater levels of Chl b per cell relative to the wild type. Conversely, TLA1 RNAi transformants had a smaller Chl antenna size for both photosystems and lower levels of Chl b per cell. Western blot analyses of the TLA1 over-expressing and RNAi transformants showed that modulation of TLA1 gene expression was paralleled by modulation in the expression of light-harvesting protein, reaction centre D1 and D2, and VIPP1 genes. Transmission electron microscopy showed that modulation of TLA1 gene expression impacts the organization of thylakoid membranes in the chloroplast. Over-expressing lines showed well-defined grana, whereas RNAi transformants possessed loosely held together and more stroma-exposed thylakoids. Cell fractionation suggested localization of the TLA1 protein in the inner chloroplast envelope and potentially in association with nascent thylakoid membranes, indicating a role in Chl antenna assembly and thylakoid membrane biogenesis. The results provide a mechanistic understanding of the Chl antenna size regulation by the TLA1 gene.

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“…Lowering the concentration does not solve the problem as it also lowers the biochemical activity of hydrogen production. Balancing the biochemical function and photosynthesis have been studied (Nakada et al 1995(Nakada et al , 1996Vasilyeva et al 1999;Kondo et al 2002Kondo et al , 2006Katsuda et al 2004;Katsuda 2008;Wakayama et al 2000;Miyake et al 1999a, b). Nakada et al (1995) examined light penetration with special interest in the alteration of the spectrum of incident light.…”

Section: Light Penetration and Effective Wavelength Regionmentioning

confidence: 98%

Hydrogen Production Using Photosynthetic Bacteria

Miyake

2014

Microbial Production

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Hydrogen production using photosynthetic bacteria is reviewed here with special reference to the contributions from Japan. Research has been focused on various aspects related to the subject, from basic to applied. The capability of biomass conversion to hydrogen was studied from the point of view of energetics. Application technologies as reactor designs have been studied extensively in Japan in the 1990s. Light penetration to the bacterial suspension was analyzed, and a method for maximizing hydrogen production from a vessel was proposed. After great problems with electricity supply, serious approaches are being carried out for realization of renewable energy systems.The combination of biologically prepared hydrogen with renewable energy such as solar cells and wind turbines is examined to stabilize the output of small-scale electric grids. The hydrogen production process is initiated by the conversion of light quanta to chemical energy. The early process of photosynthesis and the following chemical processes are still on the frontiers of science. A mysterious event of ATP synthesis at the mitochondrial membrane is also reviewed. Studies are also concerned with the structures and physical and biochemical functions of proteins in early photosynthesis. Applications of basic science on the early process of photosynthesis have been continuous in Japan with the development of photoelectric devices with Japanese cutting-edge technology of electron conductive materials as carbon nanotubes.

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Light penetration into cell suspensions of photosynthetic bacteria and relation to hydrogen production

Cited by 105 publications

References 3 publications

Integrating dark and light bio-hydrogen production strategies: towards the hydrogen economy

Integrating dark and light bio-hydrogen production strategies: towards the hydrogen economy

Modulation of the light-harvesting chlorophyll antenna size in Chlamydomonas reinhardtii by TLA1 gene over-expression and RNA interference

Hydrogen Production Using Photosynthetic Bacteria

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