Light quality and quantity affect graft union formation of tomato plants

Ahmed, Yousef; Ali, Muhammad Moaaz; Rizwan, Hafiz Muhammad; Gad, Ahmed Gomaa; Liang, Dangdi; Li, Binqi; Kalaji, Hazem M.; Wróbel, Jacek; Xu, Yong; Chen, Faxing

doi:10.1038/s41598-021-88971-5

Cited by 24 publications

(14 citation statements)

References 54 publications

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“…The expression pattern of Eucgr.A00514 (auxin transporter-like protein 4, LAX4) was downregulated in mat-callus compared to pri-callus in the two Eucalyptus species (additional file 1 ). It is consistent with the discovery of LAX3 gene in rice after grafting [ 32 ]. The dysregulation of these genes may suggest that they might play an essential role from the beginning of wounding to encourage healing and preparation for downstream rapid development.…”

Section: Resultssupporting

confidence: 92%

Gene expression programs during callus development in tissue culture of two Eucalyptus species

Zhang¹,

Li²,

Li³

et al. 2022

BMC Plant Biol

176

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Background Eucalyptus is a highly diverse genus of the Myrtaceae family and widely planted in the world for timber and pulp production. Tissue culture induced callus has become a common tool for Eucalyptus breeding, however, our knowledge about the genes related to the callus maturation and shoot regeneration is still poor. Results We set up an experiment to monitor the callus induction and callus development of two Eucalyptus species - E. camaldulensis (high embryogenic potential) and E. grandis x urophylla (low embryogenic potential). Then, we performed transcriptome sequencing for primary callus, mature callus, shoot regeneration stage callus and senescence callus. We identified 707 upregulated and 694 downregulated genes during the maturation process of the two Eucalyptus species and most of them were involved in the signaling pathways like plant hormone and MAPK. Next, we identified 135 and 142 genes that might play important roles during the callus development of E. camaldulensis and E. grandis x urophylla, respectively. Further, we found 15 DEGs shared by these two Eucalyptus species during the callus development, including Eucgr.D00640 (stem-specific protein TSJT1), Eucgr.B00171 (BTB/POZ and TAZ domain-containing protein 1), Eucgr.C00948 (zinc finger CCCH domain-containing protein 20), Eucgr.K01667 (stomatal closure-related actinbinding protein 3), Eucgr.C00663 (glutaredoxin-C10) and Eucgr.C00419 (UPF0481 protein At3g47200). Interestingly, the expression patterns of these genes displayed “N” shape in the samples. Further, we found 51 genes that were dysregulated during the callus development of E. camaldulensis but without changes in E. grandis x urophylla, such as Eucgr.B02127 (GRF1-interacting factor 1), Eucgr.C00947 (transcription factor MYB36), Eucgr.B02752 (laccase-7), Eucgr.B03985 (transcription factor MYB108), Eucgr.D00536 (GDSL esterase/lipase At5g45920) and Eucgr.B02347 (scarecrow-like protein 34). These 51 genes might be associated with the high propagation ability of Eucalyptus and 22 might be induced after the dedifferentiation. Last, we performed WGCNA to identify the co-expressed genes during the callus development of Eucalyptus and qRT-PCR experiment to validate the gene expression patterns. Conclusions This is the first time to globally study the gene profiles during the callus development of Eucalyptus. The results will improve our understanding of gene regulation and molecular mechanisms in the callus maturation and shoot regeneration.

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Section: Resultssupporting

confidence: 92%

Gene expression programs during callus development in tissue culture of two Eucalyptus species

Zhang¹,

Li²,

Li³

et al. 2022

BMC Plant Biol

176

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“…Naznin, et al 38 and Yang, et al 39 investigated in pepper seedlings and Yang et al 40 tomato seedlings, and they found that in particular the red LED light was important for producing strong seedlings. Also, Yousef and coauthors showed that a combination of R and B LED light with a high R portion was effective in producing vigorous grafted tomato seedlings 28 , 29 . In most studies a combination of R and B light was more effective in promoting plant growth and development than the use of one LED source alone.…”

Section: Discussionmentioning

confidence: 99%

“…It has been reported that different color ratios (different light spectrums) strongly influence on plants’ physiological and developmental outcomes 21 – 24 . Other researches revealed the effect of light irradiance on plant growth and development 23 , 25 – 29 and photoperiod 23 , 30 . However, little is known about the combined effect of light irradiance, quality, and photoperiod on plants’ growth, development, and physiological response.…”

Section: Introductionmentioning

confidence: 99%

Increasing the performance of Passion fruit (Passiflora edulis) seedlings by LED light regimes

Liang

Ahmed

Wei

et al. 2021

Sci Rep

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Due to progress in the industrial development of light-emitting diodes (LEDs), much work has been dedicated to understanding the reaction of plants to these light sources in recent years. In this study, the effect of different LED-based light regimes on growth and performance of passion fruit (Passiflora edulis) seedlings was investigated. Combinations of different light irradiances (50, 100, and 200 µmol m−2 s−1), quality (red, green, and blue light-emitting LEDs), and photoperiods (10 h/14 h, 12 h/12 h and 14 h/10 h light/dark cycles) were used to investigate the photosynthetic pigment contents, antioxidants and growth traits of passion fruit seedlings in comparison to the same treatment white fluorescent light. Light irradiance of 100 µmol m−2 s−1 of a 30% red/70% blue LED light combination and 12 h/12 h light/dark cycles showed the best results for plant height, stem diameter, number of leaves, internode distance, and fresh/dry shoot/root weights. 14 h/10 h light/dark cycles with the same LED light combination promoted antioxidant enzyme activities and the accumulation of phenols and flavonoids. In contrast, lower light irradiance (50 µmol m−2 s−1) had negative effects on most of the parameters. We conclude that passion fruit seedlings' optimal performance and biomass production requires long and high light irradiances with a high blue light portion.

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“…The major sites of damage owing to high temperature have been identified as carbon metabolism in the stroma and chemical signalling in thylakoid lamellae 12 . Photosynthesis is more heat sensitive as compared to dark respiration and is inhibited before the inhibition of respiration due to the plant's injury caused by high temperature 13 , 14 . High temperature makes plant tissues lose water, which makes it hard for minerals to get where they need to go 15 – 19 .…”

Section: Introductionmentioning

confidence: 99%

Heat stress mitigation in tomato (Solanum lycopersicum L.) through foliar application of gibberellic acid

Guo

Gull

Ali

et al. 2022

Sci Rep

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Phytohormones mediate physiological, morphological, and enzymatic responses and are important regulators of plant growth and development at different stages. Even though temperature is one of the most important abiotic stressors for plant development and production, a spike in the temperature may have disastrous repercussions for crop performance. Physiology and growth of two tomato genotypes ('Ahmar' and 'Roma') were studied in two growth chambers (25 and 45 °C) when gibberellic acid (GA3) was applied exogenously. After the 45 days of planting, tomato plants were sprayed with GA3 at concentrations of 25, 50, 75, and 100 mg L−1, whereas untreated plants were kept as control. Under both temperature conditions, shoot and root biomass was greatest in 'Roma' plants receiving 75 mg L−1 GA3, followed by 50 mg L−1 GA3. Maximum CO2 index, photosynthetic rate, transpiration rate, and greenness index were recorded in 'Roma' plants cultivated at 25 °C, demonstrating good effects of GA3 on tomato physiology. Likewise, GA3 enhanced the proline, nitrogen, phosphorus, and potassium levels in the leaves of both genotypes at both temperatures. Foliar-sprayed GA3 up to 100 mg L−1 alleviated the oxidative stress, as inferred from the lower concentrations of MDA and H2O2, and boosted the activities of superoxide dismutase, peroxidase, catalase. The difference between control and GA3-treated heat-stressed plants suggests that GA3 may have a function in mitigating heat stress. Overall, our findings indicate that 75 mg L−1 of GA3 is the optimal dosage to reduce heat stress in tomatoes and improve their morphological, physiological, and biochemical characteristics.

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Light quality and quantity affect graft union formation of tomato plants

Cited by 24 publications

References 54 publications

Gene expression programs during callus development in tissue culture of two Eucalyptus species

Gene expression programs during callus development in tissue culture of two Eucalyptus species

Increasing the performance of Passion fruit (Passiflora edulis) seedlings by LED light regimes

Heat stress mitigation in tomato (Solanum lycopersicum L.) through foliar application of gibberellic acid

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