Light Regulation of Genes for the Large and Small Subunits of Ribulose-Bisphosphate Carboxylase in Tobacco

Abbott, Marilyn S.; Bogorad, Lawrence

doi:10.1007/978-94-017-0519-6_106

Cited by 9 publications

(6 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The level of rftfS mRNA in plants placed in a darkened chamber declined within 10-16 h to less than 20*^0 of the level of plants maintained in the greenhouse, whereas rbc\^ mRNA content was unaffected. A similar effect of darkness on /-6("S (and lack of an effect on ricL) mRNA was observed previously in tobacco (Abott & Bogorad, 1986), amaranth (Berry, Breiding & Klessig, 1988) and potato (Fritz et al, 1991). Since 10-16 h were required for the maximum decline in rirS mRNA content, but O., caused an immediate and drastic decline in rbcS mRNA in the immature leaf, it might be suspected that Rubisco synthesis in the immature leaves of control plants continued for up to 10 h in the dark, while being severely inhibited in the O,;-treated plants.…”

Section: Discussionsupporting

confidence: 86%

O₃‐induced degradation of Rubisco protein and loss of Rubisco mRNA in relation to leaf age in Solanum tuberosum L.

Eckardt

Pell

1994

New Phytologist

View full text Add to dashboard Cite

The effect of ozone (O^) on Rubisco degradation was in\ estigated by dark incubation of potato plants {Solanum tuberosum L. cv. Norland) following exposure to charcoal-Bkered air or 0-30//I 1"' O., for 6 h. Rubisco small subunit (rbcS) mRNA le\-els declined dramatica)l> in control foliage within 10-16 h of dark incubation; thus declines in Rubisco protetn following a 48-h dark period were ascribed to proteolytic degradation. Foliage sampled from the 4th and 6th leaves from tbe apex, designated immature and mature, respecti\-e]y, showed no effect of Oô n Rubisco protein content immediateh following the exposure. Ho\ve\-er, the decline in Rubisco quantity during the dark incubation was significantly enhanced by prior treatment with O^ in tbe mature leaves. The immature lea\-es sustained a similar decline in Rubisco quantity in both t),, and control treatments. O., had a significant effect on the relative quantity of rbfS mRXA in the immature leaves (sampled immediately following tbe O., exposure). Levels of rhc?< mRNA in mature leaves and Rubisco large subunit irbc'L) mRN.\ in both leaf ages were not significantly affected by O.,. Tbere was no effect of O.^ on Rubisco quantit>-m immature or mature Iea\'es of plants maintained in the greenhouse undet a J 6 h photoperiod fot 48 h following the exposure. Thus the effect of shortterm O.^ exposure on processes affecting Rubisco synthesis and degradation may have been transient, and a more prolonged exposure would be necessary to effect a decline in Rubisco protein quantit\-tn plants grown under a 16 h photoperiod. We concluded that (),, caused enhanced degradation of Rubisco in mature lea\-es of plants induced to senesce by dark incubation following O., exposures. The potential for O^-induced reduction in synthesis of Rubisco also exists.

show abstract

Section: Discussionsupporting

confidence: 86%

O₃‐induced degradation of Rubisco protein and loss of Rubisco mRNA in relation to leaf age in Solanum tuberosum L.

Eckardt

Pell

1994

New Phytologist

View full text Add to dashboard Cite

show abstract

“…4a) were all lower in the mature leaves exposed to elevated CO2 than in the control plants. The low responsiveness of chloroplast genes to other environmental factors compared with nuclear genes has been reported by several authors (Abbott & Bogorad 1987;Piechulla & Gruissem 1987;Prioul&Reyss 1988).…”

Section: Discussionmentioning

confidence: 77%

Some relationships between the gas exchange, biochemistry and molecular biology of photosynthesis during leaf development of tomato plants after transfer to different carbon dioxide concentrations

Oosten¹,

Besford²

1995

Plant Cell & Environment

117

View full text Add to dashboard Cite

Tomato plants were exposed to four concentrations of CO2 (350, 700, 1050 or 1400 μmol CO2 mol−1) for 31 d. The light‐saturated rate of photosynthesis (A) of the unshaded fifth leaf was measured at either an ambient CO2 concentration of 350 μmol CO2 mol−1 [A (350)] or at the level of CO2 at which the plants were grown. The chloroplast protein composition and the level of transcripts of nuclear or plastid photosynthesis‐associated genes (PAGs), as well as the main carbohydrate content of the fifth leaf maintained horizontal and unshaded, were also measured during leaf development. At 60 and 95% leaf expansion, the A of high‐CO2‐grown plants measured at growth CO2 was higher than the A (350) of the plants grown at ambient CO2. However, in the fully mature leaves, A (growth CO2) declined linearly as growth CO2 concentration increased. The A (350) of plants exposed to elevated CO2 up to 60% leaf expanion had not acclimated to high CO2. At 95% leaf expansion, A (350) was lower in plants grown at high CO2. A versus CO2 (Ci) for mature leaves showed that A of the plants grown at high CO2 was lower over the entire range than that for plants grown at present ambient CO2 concentration. Lines fitted to the linear part of the A/Ci curves were concurrent at a Ci of 49μmol CO2 mol−1 and A=−1.21μmol CO2 m−2s−1. This Ct value is close to Λ* (46 μmol CO2 mol−1), the compensation point at 27°C calculated from the equation described in Brooks & Farquhar (1985, Planta 165, 397–406). This A is an estimate of respiration in the light (R1) and was not affected by acclimation to elevated CO2. Thylakoid proteins (photo‐system I core protein, D1 and D2 of the photosystem II core complex, cytochrome f) were all reduced by elevated CO2 only in the fully mature leaves (31d exposure), whereas the large and small subunits of Rubisco and Rubisco activase proteins had already declined after 22 d exposure. Transcript levels of the plastid‐encoded PAG (rbcL, psbA, psaA‐B) were reduced in the mature leaves by elevated CO2 when expressed on a total RNA basis, but they were not sensitive to elevated CO2 when expressed on a chloroplast 16S rRNA basis. However, rbcS, rca and cab mRNA transcripts were lower in the plants grown at high CO2 than in control plants after 22 d exposure when expressed on a nuclear rRNA basis. The loss of these nuclear PAGs was correlated with an accumulation of soluble sugars and starch.

show abstract

“…The absence of an effect of high CO2, Sue or Glc on the transcripts of chloroplastic genes {psa A-E, psb A) has been observed previously for rbcL . Moreover, several authors have reported the low responsiveness of chloroplastic genes to environmental factors (Abbott & Bogorad 1987;Piechulla & Gruissem 1987;Prioul & Reyss 1988). The expression of chloroplastic genes is more likely to be regulated at the translational and post-translational levels (Mullet 1988).…”

Section: Discussionmentioning

confidence: 99%

Regulation of the expression of photosynthetic nuclear genes by CO₂ is mimicked by regulation by carbohydrates: a mechanism for the acclimation of photosynthesis to high CO₂?

Oosten¹,

Wilkins²,

Besford³

1994

Plant Cell & Environment

150

View full text Add to dashboard Cite

The abundance of transcripts of cab‐7 and cab‐3C, which code for the chlorophyll a/b binding proteins of the light‐harvesting complexes I and II, respectively, and the abundance of transcripts of Rca, which encodes Rubisco activase, were reduced in tomato plants exposed to high CO2 for up to 9d, whereas the abundance of mRNA from psa A–psa B and psb A, which encode the proteins of the core complex of PSI and the D1 protein of PSII, respectively, and the abundance of glycolate oxidase, which is involved in photorespiration, were not affected. However, the abundance of the transcript for the B subunit of ADP‐glucose pyrophosphorylase was increased after 1 d at elevated CO2. The chlorophyll a/b ratio decreased significantly over 9 d of exposure to elevated CO2. The responses of the nuclear genes to high CO2 were enhanced when leaves were detached so as to deprive them of any major sink. The responses of these transcripts to high CO2 were mimicked when sucrose or glucose was supplied to the leaf tissue, whereas acetate or sorbitol had no effect. Carbohydrate analyses of leaves grown in high CO2 or supplied with sucrose revealed that major increases occurred in the amount of glucose and fructose. Based on these and other published data, a molecular model involving the repression or activation of the transcription of nuclear genes coding for chloroplast proteins by photosynthetic end‐products is proposed to account for photosynthetic acclimation to high CO2 in tomato plants and other species.

show abstract

Light Regulation of Genes for the Large and Small Subunits of Ribulose-Bisphosphate Carboxylase in Tobacco

Cited by 9 publications

References 6 publications

O₃‐induced degradation of Rubisco protein and loss of Rubisco mRNA in relation to leaf age in Solanum tuberosum L.

O₃‐induced degradation of Rubisco protein and loss of Rubisco mRNA in relation to leaf age in Solanum tuberosum L.

Some relationships between the gas exchange, biochemistry and molecular biology of photosynthesis during leaf development of tomato plants after transfer to different carbon dioxide concentrations

Regulation of the expression of photosynthetic nuclear genes by CO₂ is mimicked by regulation by carbohydrates: a mechanism for the acclimation of photosynthesis to high CO₂?

Contact Info

Product

Resources

About

Light Regulation of Genes for the Large and Small Subunits of Ribulose-Bisphosphate Carboxylase in Tobacco

Cited by 9 publications

References 6 publications

O3‐induced degradation of Rubisco protein and loss of Rubisco mRNA in relation to leaf age in Solanum tuberosum L.

O3‐induced degradation of Rubisco protein and loss of Rubisco mRNA in relation to leaf age in Solanum tuberosum L.

Some relationships between the gas exchange, biochemistry and molecular biology of photosynthesis during leaf development of tomato plants after transfer to different carbon dioxide concentrations

Regulation of the expression of photosynthetic nuclear genes by CO2 is mimicked by regulation by carbohydrates: a mechanism for the acclimation of photosynthesis to high CO2?

Contact Info

Product

Resources

About

O₃‐induced degradation of Rubisco protein and loss of Rubisco mRNA in relation to leaf age in Solanum tuberosum L.

O₃‐induced degradation of Rubisco protein and loss of Rubisco mRNA in relation to leaf age in Solanum tuberosum L.

Regulation of the expression of photosynthetic nuclear genes by CO₂ is mimicked by regulation by carbohydrates: a mechanism for the acclimation of photosynthesis to high CO₂?