Light Represses Conidiation in Koji MoldAspergillus oryzae

Hatakeyama, Riko; Nakahama, Tomoyuki; Higuchi, Yujiro; Kitamoto, Katsuhiko

doi:10.1271/bbb.60713

Cited by 26 publications

(33 citation statements)

References 18 publications

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“…2). As previously reported by Hatakeyama et al (2007), RIB 40 produced more conidia under the dark condition (Fig. 2).…”

Section: Resultssupporting

confidence: 89%

“…No significant difference was observed in gene expression levels of these three genes between light and dark conditions. In the previous report of Hatakeyama et al, the expression level of brlA between light and dark conditions showed no significant differences (Hatakeyama et al 2007). From these results, we could not explain why RIB 1187 showed a different light response to RIB40.…”

Section: Resultscontrasting

confidence: 55%

“…Kumagai (1978) classified the mode of light response in conidiophore induction and conidial development of several fungi including plant pathogens into three categories. Hatakeyama et al (2007) investigated A. oryzae RIB 40 (a genome sequenced strain of A. oryzae), and found that it conserved light signaling molecules and can respond to light, but in a manner opposite to that of A. nidulans (as A. oryzae produces more conidia under the dark condition than under the light condition). In this paper, we screened A. oryzae strain RIB 1187 that showed a different light response to RIB 40.…”

Section: Introductionmentioning

confidence: 99%

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Aspergillus Oryzae Strain with Improved Conidiation after Light Stimulation

Murthy

Sano

Hattori

et al. 2018

JARQ

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The influence of light on the development of asexual spores is an example to elucidate the effect of environmental factors on fungi. Well-studied fungus Aspergillus nidulans produces more conidia under the light condition than under the dark condition, whereas RIB 40, a standard strain of A. oryzae, produces more conidia under the dark condition. In this study, we found that an A. oryzae strain (RIB 1187) responded positively to incubation under light and resulted in better conidia count as compared to that in the dark. We studied the gene expression of RIB 1187 and found that four homologous genes of A. nidulans (up-regulated genes by light) were also up-regulated in RIB 1187 under the light condition.

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“…2). As previously reported by Hatakeyama et al (2007), RIB 40 produced more conidia under the dark condition (Fig. 2).…”

Section: Resultssupporting

confidence: 89%

Section: Resultscontrasting

confidence: 55%

Section: Introductionmentioning

confidence: 99%

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Aspergillus Oryzae Strain with Improved Conidiation after Light Stimulation

Murthy

Sano

Hattori

et al. 2018

JARQ

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“…This implies that loss/gain of function of FluG in asexual development has occurred at least two independent times in the evolution of aspergilli. We also showed that asexual development does not depend on light in the case of A. niger, while it preferentially takes place in the light in the case of A. nidulans (Purschwitz et al 2008) and is repressed by white and red light in A. oryzae (Hatakeyama et al 2007). Multiple copies of amyR were introduced in A. niger to assess whether over-expression of this regulator would impact production levels of glucoamylase and its spatial release in colonies.…”

Section: Discussionmentioning

confidence: 81%

FluG affects secretion in colonies of Aspergillus niger

Wang

Krijgsheld

Hulsman

et al. 2014

Antonie van Leeuwenhoek

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Colonies of Aspergillus niger are characterized by zonal heterogeneity in growth, sporulation, gene expression and secretion. For instance, the glucoamylase gene glaA is more highly expressed at the periphery of colonies when compared to the center. As a consequence, its encoded protein GlaA is mainly secreted at the outer part of the colony. Here, multiple copies of amyR were introduced in A. niger. Most transformants over-expressing this regulatory gene of amylolytic genes still displayed heterogeneous glaA expression and GlaA secretion. However, heterogeneity was abolished in transformant UU-A001.13 by expressing glaA and secreting GlaA throughout the mycelium. Sequencing the genome of UU-A001.13 revealed that transformation had been accompanied by deletion of part of the fluG gene and disrupting its 3 0 end by integration of a transformation vector. Inactivation of fluG in the wild-type background of A. niger also resulted in breakdown of starch under the whole colony. Asexual development of the DfluG strain was not affected, unlike what was previously shown in Aspergillus nidulans. Genes encoding proteins with a signal sequence for secretion, including part of the amylolytic genes, were more often downregulated in the central zone of maltose-grown DfluG colonies and upregulated in the intermediate part and periphery when compared to the wild-type. Together, these data indicate that FluG of A. niger is a repressor of secretion.Keywords Fungus Á Conidiogenesis Á Asexual development Á Aspergillus fluG Á Secretion Á Heterogeneity Electronic supplementary material The online version of this article

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“…Generally, the conidium of A. oryzae show yellow-green color [4], but it sometimes appears in other anomalous color (such as white, or brown) in proliferation process. It has been reported that some genes are involved in formation of conidial pigments in Aspergillus species.…”

Section: Introductionmentioning

confidence: 99%

Comparative Analysis of Aspergillus oryzae with Normal and Abnormal Color Conidia

Lin

Wen-biao

et al. 2013

Indian J Microbiol

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This study focuses on the characteristic of strains with anomalous color conidium and compares with normal color conidium. Comparative analysis of enzymes activity and extracellular proteins revealed that A. oryzae with anomalous color conidium was not different from the strain with normal color conidium. In addition, A. oryzae with anomalous color conidium could not influence the palatability and quality of the soy sauce. These findings provide an insight into A. oryzae with anomalous color conidium.

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Light Represses Conidiation in Koji MoldAspergillus oryzae

Cited by 26 publications

References 18 publications

<i>Aspergillus Oryzae</i> Strain with Improved Conidiation after Light Stimulation

<i>Aspergillus Oryzae</i> Strain with Improved Conidiation after Light Stimulation

FluG affects secretion in colonies of Aspergillus niger

Comparative Analysis of Aspergillus oryzae with Normal and Abnormal Color Conidia

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