Light-sheet functional imaging in fictively behaving zebrafish

Vladimirov, Nikita; Mu, Yu; Kawashima, Takashi; Bennett, Davis; Yang, Chao-Tsung; Looger, Loren L.; Keller, Philipp J.; Freeman, Jeremy; Ahrens, Misha B.

doi:10.1038/nmeth.3040

Cited by 305 publications

(316 citation statements)

References 9 publications

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“…During recording, the larvae exhibited different speeds of movement and even inversion of moving direction. Combined with genetically encoded calcium sensors [15], our SPIM system could be potentially used to follow neuronal activity in these freely behaving larvae. …”

Section: Imaging Of Moving Drosophila Larvamentioning

confidence: 99%

Open-top selective plane illumination microscope for conventionally mounted specimens

et al. 2015

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Abstract:We have developed a new open-top selective plane illumination microscope (SPIM) compatible with microfluidic devices, multi-well plates, and other sample formats used in conventional inverted microscopy. Its key element is a water prism that compensates for the aberrations introduced when imaging at 45 degrees through a coverglass. We have demonstrated its unique high-content imaging capability by recording Drosophila embryo development in environmentally-controlled microfluidic channels and imaging zebrafish embryos in 96-well plates. We have also shown the imaging of C. elegans and moving Drosophila larvae on coverslips. Hillman, "Swept confocally-aligned planar excitation (SCAPE) microscopy for high speed volumetric imaging of behaving organisms," Nat. Photonics 9(2), 113-119 (2015 an open-source platform for biological-image analysis," Nat. Methods 9(7), 676-682 (2012).

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Section: Imaging Of Moving Drosophila Larvamentioning

confidence: 99%

Open-top selective plane illumination microscope for conventionally mounted specimens

et al. 2015

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“…Fluorescence from the sheet can be imaged simultaneously to a camera (e.g., 4.2 megapixel sCMOS cameras are sensitive enough for functional imaging with frames rates of 100 fps). With high speed and expanded volumes, light sheet microscopes can follow developmental processes in small organisms over several days [6,[18][19][20], though have so far been limited to transparent samples like C. elegans, zebrafish larvae, and Drosophila larvae.…”

Section: Light Microscopy Of Volumesmentioning

confidence: 99%

Correlating 3D light to 3D electron microscopy for systems biology

Collinson

Carroll

Hoogenboom

2017

Current Opinion in Biomedical Engineering

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“…Furthermore, fast volumetric imaging could simultaneously extract information from fluorescent molecular sensors together with "biomechanical" data from freely moving zebrafish larvae exhibiting unrestrained behavior; this may complement virtual environment approaches that investigate neural activity during fictive behavior in immobilized zebrafish larvae. 11 Lately, technical advances have been made in acquisition and reconstruction of light field microscopy data [12][13][14] and three light field microscopes have been custom-built in different laboratories specifically to image neuronal activity in immobilized Caenorhabditis elegans worms and zebrafish larvae. 8,14,15 Despite the generous provision of open access documentation on light field microscopy hardware and open source software, 16,17 an "off-the shelf" light field camera system would certainly help to disseminate this comparably straightforward and compact imaging technology for widespread biological use.…”

Section: Introductionmentioning

confidence: 99%

Calcium neuroimaging in behaving zebrafish larvae using a turn-key light field camera

et al. 2015

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Abstract. Reconstructing a three-dimensional scene from multiple simultaneously acquired perspectives (the light field) is an elegant scanless imaging concept that can exceed the temporal resolution of currently available scanning-based imaging methods for capturing fast cellular processes. We tested the performance of commercially available light field cameras on a fluorescent microscopy setup for monitoring calcium activity in the brain of awake and behaving reporter zebrafish larvae. The plenoptic imaging system could volumetrically resolve diverse neuronal response profiles throughout the zebrafish brain upon stimulation with an aversive odorant. Behavioral responses of the reporter fish could be captured simultaneously together with depth-resolved neuronal activity. Overall, our assessment showed that with some optimizations for fluorescence microscopy applications, commercial light field cameras have the potential of becoming an attractive alternative to custom-built systems to accelerate molecular imaging research on cellular dynamics. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Light-sheet functional imaging in fictively behaving zebrafish

Cited by 305 publications

References 9 publications

Open-top selective plane illumination microscope for conventionally mounted specimens

Open-top selective plane illumination microscope for conventionally mounted specimens

Correlating 3D light to 3D electron microscopy for systems biology

Calcium neuroimaging in behaving zebrafish larvae using a turn-key light field camera

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