Light-Sheet Microscopy for Surface Topography Measurements and Quantitative Analysis

Xu, Zhanpeng; Forsberg, Erik; Guo, Yang; Cai, Fuhong; He, Sailing

doi:10.3390/s20102842

Cited by 7 publications

(3 citation statements)

References 31 publications

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“…Additionally, by designing optical structure and selecting special components, the wavelength band of the MMHI can be expanded to near-IR band for in-vivo detection [27]. Furthermore, combining the hyperspectral imaging technology with 3D microscopic inspection methods [28], the 2D hyperspectral image may be extended to 3D hyperspectral topography. We believe that the MMHI will have more applications in multi-functional bio detections in the future.…”

Section: Discussionmentioning

confidence: 99%

Multi-mode Microscopic Hyperspectral Imager for the Sensing of Biological Samples

Jiang

2020

Applied Sciences

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In this work, we develop a multi-mode microscopic hyperspectral imager (MMHI) for the detection of biological samples in transmission imaging, reflection imaging and fluorescence mode. A hyperspectral image cube can be obtained with 5 μm spatial resolution and 3 nm spectral resolution through push-broom line scanning. To avoid possible shadows produced by the high magnification objective with a short working distance, two illumination patterns are designed to ensure the co-axiality of the illumination and detection. Three experiments for the detection of zebrafish and fingerprints and the classification of disaster-causing microalgae verify the good capability and functionality of the system. Based on the detected spectra, we can observe the impacts of β-carotene and melanin in zebrafish, hemoglobin in the fingertip, and chlorophyll in microalgae, respectively. Multi-modes can be switched freely according to the application requirement and characteristics of different samples, like transmission mode for the transparent/translucent sample, reflection mode for the opaque sample and fluorescence mode for the fluorescent sample. The MMHI system also has strong potential for the non-invasive and high-speed sensing of bio or clinical samples.

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Section: Discussionmentioning

confidence: 99%

Multi-mode Microscopic Hyperspectral Imager for the Sensing of Biological Samples

Jiang

2020

Applied Sciences

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“…This mainly depends on the following aspects: some protein biomarkers exist at low abundance in the early stages and are difficult to monitor, especially in complex environments, such as various body fluids, daily foods, and waste fluids [3][4][5]. Currently, many ultrasensitive immune technologies have been developed, such as electrochemical immunosensor [6], single molecule detection [7], microfluidic technology [8], mass spectrometry immunoassay [9,10], and hyperspectral sensing imaging [11,12]. However, the high sensitivity of these technologies, such as computed tomography (CT) diagnosis and nucleic acid screening of COVID-19 [13,14], traditional cultivation and identification of pathogenic microorganisms susceptible to infection in infants [15], and imaging examination of infectious diseases [16], comes at the price of expensive instruments and costs, complex technical operations, and longer detection times, which cannot meet the needs of fast and highthroughput detection.…”

Section: Introductionmentioning

confidence: 99%

Lateral Flow Immunoassay Strip Based on Confocal Raman Imaging for Ultrasensitive and Rapid Detection of COVID-19 and Bacterial Biomarkers

Zhang,

Yang,

2023

PIER M

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Rapid and sensitive analysis of proteins in complex biological environments is crucial for the screening and defense against infectious diseases. Here, we show that the lateral flow immunoassay strip based on confocal Raman imaging can achieve immune analysis at pM and ∼ 10 4 cfu/mL molecular level for the rapid detection of COVID-19 virus and bacteria. Fluorescent dyes of Alexa 647 were used as Raman markers in the Raman silent region of 1800 cm −1 and 2800 cm −1 , and colloidal gold nanospheres were used to enhance the Raman signal. Raman imaging was performed with our self-developed confocal Raman microscopy for COVID-19 and Escherichia coli O157: H7 on lateral flow immunoassay strip. Compared to traditional colloidal gold test strips, the sensitivity of this technology has been significantly improved. This work will promote the widespread application of surface enhanced Raman detection for bacteria and virus, which is of great significance for in vitro screening and disease diagnosis.

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“…Another way is position-based, including stereo vision, laser range scanning, and structured illumination, etc. [6][7][8][9][10][11][12][13]. It scans a single line or a plane at a time and utilizes the principle of triangulation to derive the depth of those points.…”

Section: Introductionmentioning

confidence: 99%

A Parameter-Free Calibration Process for a Scheimpflug Lidar for Volumetric Profiling

Luo¹,

Chen²,

Xu³

et al. 2020

PIER

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Light-Sheet Microscopy for Surface Topography Measurements and Quantitative Analysis

Cited by 7 publications

References 31 publications

Multi-mode Microscopic Hyperspectral Imager for the Sensing of Biological Samples

Multi-mode Microscopic Hyperspectral Imager for the Sensing of Biological Samples

Lateral Flow Immunoassay Strip Based on Confocal Raman Imaging for Ultrasensitive and Rapid Detection of COVID-19 and Bacterial Biomarkers

A Parameter-Free Calibration Process for a Scheimpflug Lidar for Volumetric Profiling

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