Light sources on the germination and initial in vitro establishment of Schomburgkia crispa Lindl., a species of the Brazilian Cerrado

Sorgato, José Carlos; Mudolon, Erick Dutra; Guimarães, Fernando Figueiredo; Soares, Jackeline Schultz; Ribeiro, Luan Marlon

doi:10.1590/0103-8478cr20190022

Cited by 3 publications

(1 citation statement)

References 15 publications

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“…The results found in this study show that the light condition of the cultivation room for Orchidaceae species are, in addition to speciesspecific, also related to the stage of development of the plant. The results found in the literature differ according to the stage of seedlings used (Freitas et al 2021;Sorgato et al 2021a).…”

Section: In Vitro Growth -120 Daysmentioning

confidence: 90%

In vitro and ex vitro production of Schomburgkia crispa: effect of flask sealing systems and different light sources

Ramos,

Ribeiro,

Nunes

et al. 2023

Rodriguésia

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The extraction of native orchids from natural habitats is relevant for the reduction of populations in the Cerrado biome, making it necessary to establish practices aiming their production both for reintroduction and commercialization. The objective here is to evaluate light sources and sealing systems on the in vitro and ex vitro growth of Schomburgkia crispa. Two flask sealing systems were tested: conventional (CSS) and with gas exchange (SSGE), and eight light sources: FL1-100% white LED, FL2-100% blue LED, FL3-100% red LED, FL4-50% white + 25% red + 25% blue LED, FL5-50% red + 50% blue LED, FL6-25% red + 75% blue LED, FL7-75% red + 25% blue LED, and FL8- with fluorescent lamp, with five replications in each treatment. A completely randomized design was adopted with a 2x8 factorial scheme (vial sealing system x light sources). After 120 days of cultivation in vitro and 180 days ex vitro, the plants were evaluated as for number of leaves, roots and shoots, plant height, pseudobulb diameter, length of the largest root, largest leaf, and fresh mass. For the in vitro growth, the use of SSGE together with the light sources blue and red favors the cultivation of S. crispa. For the ex vitro growth, the cultivation in vitro in SSGE together with FL4 affects the acclimatization of plants.

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Section: In Vitro Growth -120 Daysmentioning

confidence: 90%

In vitro and ex vitro production of Schomburgkia crispa: effect of flask sealing systems and different light sources

Ramos,

Ribeiro,

Nunes

et al. 2023

Rodriguésia

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Synergizing LED Technology and Hydropriming for Intelligent Modeling and Mathematical Expressions to Optimize Chickpea Germination and Growth Indices

Aasim,

Akin,

Ali

2024

J Plant Growth Regul

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The influence of hydropriming and Light Emitting Diodes (LED) on germination and growth indices, followed by optimizing and validation via artificial intelligence-based models was carried out in this research. White LEDs (W-LEDs) were more effective by yielding the most effective growth indices, such as mean germination time (MGT) (1.11 day), coefficient of variation of germination time (CVt) (20.72%), mean germination rate (MR) (0.81 day−1), uncertainty (U) (0.40 bit), and synchronization (Z values) (0.79); the optimum MGT (1.09 day), CVt (15.97%), MR (0.77 day−1), U (0.32 bit), and Z (0.55) values were found after 2 h of hydropriming, which was responsible for all efficient growth indicators. W-LEDs with 1 h hydropriming proved to be the ideal LED and hydropriming combination. Results on growth indices for in vitro seedlings were completely different from those on germination indices, and the most desirable germination indices were linked to red LEDs (R-LEDs). Whereas 4 h hydropriming was most effective for the post-germination process. Pareto charts, normal plots, contour plots, and surface plots were created to optimize the input variables. Finally, the data were predicted using Arificial Neural Network (ANN) inspired multilayer perceptron (MLP) and machine learning-based random forest (RF) algorithms. For both models, plant height was correlated with maximum R2 values. Whereas, all output variables had relatively low mean absolute error (MAE), mean square error (MSE), and mean absolute percentage error (MAPE) scores, indicating that both models performed well. The results of this investigation disclosed a link between certain LEDs and hydropriming treatment for in vitro germination indices and plant growth. Graphical Abstract Graphical presentation of actual and predicted values for germination indices in chickpea

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Effect of LED Lighting on Physical Environment and Microenvironment on In Vitro Plant Growth and Morphogenesis: The Need to Standardize Lighting Conditions and Their Description

Barceló-Muñóz

Barceló

Calderón

2021

Plants

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In the last decades, lighting installations in plant tissue culture have generally been renewed or designed based on LED technology. Thanks to this, many different light quality advances are available but, with their massive implementation, the same issue is occurring as in the 1960s with the appearance of the Grolux (Sylvania) fluorescent tubes: there is a lack of a methodological standardization of lighting. This review analyzes the main parameters and variables that must be taken into account in the design of LED-based systems, and how these need to be described and quantified in order to homogenize and standardize the experimental conditions to obtain reproducible and comparable results and conclusions. We have designed an experimental system in which the values of the physical environment and microenvironment conditions and the behavior of plant tissue cultures maintained in cabins illuminated with two lighting designs can be compared. Grolux tubes are compared with a combination of monochromatic LED lamps calibrated to provide a spectral emission, and light irradiance values similar to those generated by the previous discharge lamps, achieving in both cases wide uniformity of radiation conditions on the shelves of the culture cabins. This study can help to understand whether it is possible to use LEDs as one standard lighting source in plant tissue culture without affecting the development of the cultures maintained with the previously regulated protocols in the different laboratories. Finally, the results presented from this caparison indicate how temperature is one of the main factors that is affected by the chosen light source.

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Light sources on the germination and initial in vitro establishment of Schomburgkia crispa Lindl., a species of the Brazilian Cerrado

Cited by 3 publications

References 15 publications

In vitro and ex vitro production of Schomburgkia crispa: effect of flask sealing systems and different light sources

In vitro and ex vitro production of Schomburgkia crispa: effect of flask sealing systems and different light sources

Synergizing LED Technology and Hydropriming for Intelligent Modeling and Mathematical Expressions to Optimize Chickpea Germination and Growth Indices

Effect of LED Lighting on Physical Environment and Microenvironment on In Vitro Plant Growth and Morphogenesis: The Need to Standardize Lighting Conditions and Their Description

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