Light-tunable thermoresponsive behavior of branched polyethylenimine derivatives in water

Mok, Yeongbong; Noh, Minwoo; Kim, Gyu Chan; Song, Youngjun; Kim, Heejin; Kim, Seulah; Yi, Sihyeong; Seo, Ji Hun; Lee, Yan

doi:10.1016/j.polymer.2016.11.014

Cited by 6 publications

(7 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, at lower concentrations, larger temperature changes were required to promote chain aggregation. 38 A similar trend was also observed in the case of polymer P3. As shown in Figure S3, the T cp raised from 40 to 72 °C and the phase transition became sharper when the concentration of P3 increased from 0.05 to 0.5 wt %.…”

Section: ■ Results and Discussionsupporting

confidence: 76%

See 1 more Smart Citation

Design of a UCST Polymer with Strong Hydrogen Bonds and Reactive Moieties for Facile Polymer–Protein Hybridization

Sun

et al. 2022

Biomacromolecules

View full text Add to dashboard Cite

Polymer–protein hybrids have been extensively used in biomedical fields. Polymers with upper critical solution temperature (UCST) behaviors can form a hydrated coacervate phase below the cloud point (T cp), providing themselves the opportunity to directly capture hydrophilic proteins and form hybrids in aqueous solutions. However, it is always a challenge to obtain a UCST polymer that could aggregate at a high temperature at a relatively low concentration and also efficiently bind with proteins. In this work, a UCST polymer reactive with proteins was designed, and its temperature responsiveness and protein-capture ability were investigated in detail. The polymer was synthesized by the reversible addition–fragmentation chain transfer (RAFT) polymerization of acrylamide (AAm) and N-acryloxysuccinimide (NAS). Interestingly, taking advantage of the partial hydrolysis of NAS into acrylic acid (AAc), the obtained P(AAm-co-NAS-co-AAc) polymer exhibited an excellent UCST behavior and possessed good protein-capture ability. It showed a relatively higher T cp (81 °C) at a lower concentration (0.1 wt %) and quickly formed polymer–protein hybrids with high protein loading and without losing protein bioactivity, and both the polymer and polymer–protein nanoparticles showed good cytocompatibility. All the findings are attributed to the unique structure of the polymer, which provided not only the strong and stable hydrogen bonds but also the quick and mild reactivity. The work offers an easy and mild strategy for polymer–protein hybridization directly in aqueous solutions, which may find applications in biomedical fields.

show abstract

Section: ■ Results and Discussionsupporting

confidence: 76%

“…This is because large aggregates were formed more quickly at higher concentrations than at lower concentrations. Thus, at lower concentrations, larger temperature changes were required to promote chain aggregation . A similar trend was also observed in the case of polymer P3.…”

Section: Resultsmentioning

confidence: 99%

Design of a UCST Polymer with Strong Hydrogen Bonds and Reactive Moieties for Facile Polymer–Protein Hybridization

Sun

et al. 2022

Biomacromolecules

View full text Add to dashboard Cite

show abstract

“…As discussed before, the ability to drive 4F-AZO-PEO complexation and self-assembly above and below its cloud point using visible light in water advances the field of photodissociable systems by offering full aqueous solubility, all-visible photoswitching, high cis stability, and well-separated cloud points that straddle room temperature. The abundant reports [73][74][75][76][77][78] of traditional azobenzene assemblies used for various applications, including biology, rely on UV light even though the risk of UV light to cells is well known. [28] The few reports of visibleswitchable azobenzene complexes [14,57] show smaller differences in binding constants of cis and trans with -CD than in this work, and do not explore self-assembly behavior of the azobenzene species itself.…”

Section: Discussionmentioning

confidence: 99%

“…A turbid solution (C = 10 mM) can be reversibly rendered limpid by triggering the cis to trans switch of the azo moieties using blue light irradiation (or inversely green light) at room temperature (Figure 6c). Such photoswitchable aggregation/dissolution has been investigated in traditional azobenzenecontaining polymeric systems intended as smart delivery materials, [73] aggregation-induced fluorescent probes, [74] logic circuits, [75] and nanoparticle absorption modulators for sensing/therapy. [76] These reports [73][74][75][76][77][78] of traditional azobenzene assemblies used for various applications, including biology, rely on UV light even though the risk of UV light to cells is well known.…”

Section: Lower Critical Solution Temperature In Aqueous Solutionmentioning

confidence: 99%

“…Such photoswitchable aggregation/dissolution has been investigated in traditional azobenzenecontaining polymeric systems intended as smart delivery materials, [73] aggregation-induced fluorescent probes, [74] logic circuits, [75] and nanoparticle absorption modulators for sensing/therapy. [76] These reports [73][74][75][76][77][78] of traditional azobenzene assemblies used for various applications, including biology, rely on UV light even though the risk of UV light to cells is well known. [28] The few reports of visible-switchable azobenzene complexes [14,57] show smaller differences in binding constants of cis and trans with -CD than in this work, and do not explore self-assembly behavior of the azobenzene species itself.…”

Section: Lower Critical Solution Temperature In Aqueous Solutionmentioning

confidence: 99%

See 1 more Smart Citation