Lighting-Up the Dynamics of Telomerization and DNA Replication by CdSe−ZnS Quantum Dots

Patolsky, Fernando; Gill, Ron; Weizmann, Yossi; Mokari, Taleb; Banin, Uri; Willner, Itamar

doi:10.1021/ja035848c

Cited by 348 publications

(287 citation statements)

References 12 publications

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“…It was also minimalistic design with no additional requirement for purification. conjugates to monitor the DNA replication [106]. The 'donor' (QD-DNA) conjugates were incubated in a soup of dNTPs (wherein dUTP was labelled with acceptor fluorophore) and telomerase.…”

Section: Biosensing Applicationmentioning

confidence: 99%

Quantum dots–DNA bioconjugates: synthesis to applications

et al. 2016

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Semiconductor nanoparticles particularly quantum dots (QDs) are interesting alternatives to organic fluorophores for a range of applications such as biosensing, imaging and therapeutics. Addition of a programmable scaffold such as DNA to QDs further expands the scope and applicability of these hybrid nanomaterials in biology. In this review, the most important stages of preparation of QD-DNA conjugates for specific applications in biology are discussed. Special emphasis is laid on (i) the most successful strategies to disperse QDs in aqueous media, (ii) the range of different conjugation with detailed discussion about specific merits and demerits in each case, and (iii) typical applications of these conjugates in the context of biology.

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Section: Biosensing Applicationmentioning

confidence: 99%

Quantum dots–DNA bioconjugates: synthesis to applications

et al. 2016

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“…13 FRET in QD/ DNA/organic dye conjugates has been used to study DNA hybridization, 14,15 cleavage, 16 and replication. 17 Most of the reported approaches required covalent conjugation of DNA to the QD surface (e.g., by EDC/NHS coupling reaction), which often decreases the colloidal stability and/or the emission intensity of QDs. 18,19 This limitation can be overcome by the use of a sensing platform relying on the electrostatic interaction of dye-labeled DNA with the oppositely charged QD surface 20 in a similar fashion as in conducting polymer-based detection assays.…”

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confidence: 99%

Cascaded FRET in Conjugated Polymer/Quantum Dot/Dye-Labeled DNA Complexes for DNA Hybridization Detection

et al. 2009

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A s the human genome project has uncovered the full sequence of human genomes and the postgenome technologies have been rapidly developed, DNA hybridization detection has become increasingly important. 1 A variety of DNA detection formats are now available, including both heterogeneous assays with surface-bound array-based probes and homogeneous solution-based detection schemes. 2 A number of those rely on fluorescence signal and, in particular, on fluorescence (or Fö rster) resonant energy transfer (FRET). 3 Both conjugated polymers and semiconductor nanocrystals (colloidal quantum dots, QDs) have been suggested for use as fluorescent entities in DNA detection assays. 4Ϫ12 Assays based on watersoluble cationic conjugated polymers allow one to utilize a simple binding strategy to DNA through electrostatic interactions with ionic side groups and benefit from the lightharvesting properties along the polymer backbone. 4Ϫ8 QDs have been increasingly used as donors in FRET-related studies, including optical DNA diagnostics. 9Ϫ12 QDs are photostable, highly efficient fluorophores with a strong band gap luminescence tunable by size as a result of the quantum confinement effect. 13 FRET in QD/ DNA/organic dye conjugates has been used to study DNA hybridization, 14,15 cleavage, 16 and replication. 17 Most of the reported approaches required covalent conjugation of DNA to the QD surface (e.g., by EDC/NHS coupling reaction), which often decreases the colloidal stability and/or the emission intensity of QDs. 18,19 This limitation can be overcome by the use of a sensing platform relying on the electrostatic interaction of dye-labeled DNA with the oppositely charged QD surface 20 in a similar fashion as in conducting polymer-based detection assays. 4Ϫ8 In this paper, we propose a simultaneous use of conjugated polymers and semiconductor QDs for DNA hybridization detection, which can potentially combine advantages of both light-harvesting and DNA-binding properties of water-soluble polymers with photostability, lightharvesting ability, and FRET donor property of QDs. A blue-emitting conjugated polymer with positively charged side chains electrostatically self-assembles on the negatively charged surface of red-emitting CdTe QDs. The so-formed hybrid complex has a net positive charge and thus attracts negatively charged dye-labeled DNA molecules (Scheme 1). The water-soluble, cationic, conjugated polymer, poly[9,9-bis(3=-((N,N-

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“…In fact, clever assays for the recognition of various analytes are starting to be designed on the basis of energy transfer processes (18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34). Instead, mechanisms based on electron transfer still remain to be explored (35,36).…”

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confidence: 99%

A mechanism to signal receptor–substrate interactions with luminescent quantum dots

Yildiz

Tomasulo

Raymo

2006

Proc. Natl. Acad. Sci. U.S.A.

136

106

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Semiconductor quantum dots are becoming valuable analytical tools for biomedical applications. Indeed, their unique photophysical properties offer the opportunity to design luminescent probes for imaging and sensing with unprecedented performance. In this context, we have identified operating principles to transduce the supramolecular association of complementary receptor-substrate pairs into an enhancement in the luminescence of sensitive quantum dots. Our mechanism is based on the electrostatic adsorption of cationic quenchers on the surface of anionic quantum dots. The adsorbed quenchers suppress efficiently the emission character of the associated nanoparticles on the basis of photoinduced electron transfer. In the presence of target receptors able to bind the quenchers and prevent electron transfer, however, the luminescence of the quantum dots is restored. Thus, complementary receptor-substrate pairs can be identified with luminescence measurements relying on our design logic. In fact, we have demonstrated with a representative example that our protocol can be adapted to signal protein-ligand interactions.electron transfer ͉ luminescent chemosensors ͉ nanoparticles ͉ proteinligand interactions S emiconductor quantum dots are inorganic nanoparticles with remarkable photophysical properties (1-5). In particular, their one-and two-photon absorption cross-sections, luminescence lifetimes, and photobleaching resistances are significantly greater than those of conventional organic fluorophores. Furthermore, their broad absorption bands extend continuously from the UV to the visible region of the electromagnetic spectrum and, therefore, offer a vast selection of possible excitation wavelengths. Instead, their narrow emission bands can be positioned precisely within the visible and near-infrared regions with fine adjustments of their physical dimensions. In fact, pools of quantum dots with different diameters can be designed to emit in parallel at different wavelengths after excitation at a single wavelength, offering the opportunity to implement unprecedented multichannel assays.Organic dyes do not offer the unique collection of attractive photophysical properties associated with semiconductor quantum dots. Indeed, it is becoming apparent that these inorganic nanoparticles can complement, if not replace, their organic counterparts in a diversity of biomedical applications (6-12). Nonetheless, decades of intensive investigations on the structure and properties of organic chromophores have indicated valuable strategies to design sensitive fluorescent probes able to signal the presence of target analytes with changes in emission intensity (13-16). Their operating principles generally rely on the covalent connection of a fluorescent component to a receptor unit. The receptor is engineered to quench the emission of the fluorophore on the basis of either electron or energy transfer. The supramolecular association of the receptor with a complementary analyte, however, suppresses the quenching mechanism and leads to a si...

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Lighting-Up the Dynamics of Telomerization and DNA Replication by CdSe−ZnS Quantum Dots

Abstract: CdSe-ZnS core-shell quantum dots (QDs) act as photochemical centers for lighting-up the dynamics of telomerization or DNA replication.

Cited by 348 publications

References 12 publications

Quantum dots–DNA bioconjugates: synthesis to applications

Quantum dots–DNA bioconjugates: synthesis to applications

Cascaded FRET in Conjugated Polymer/Quantum Dot/Dye-Labeled DNA Complexes for DNA Hybridization Detection

A mechanism to signal receptor–substrate interactions with luminescent quantum dots

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